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干细胞新闻贴，初次发帖，先看一下是否合符要求。
/ W% @& x4 ~/ {% T% T2 u( p1.说明：原文来源自 J Neurooncol 由干细胞之家新闻小组成员jiandong2001翻译（转帖请注明）
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2.翻译内容：最近的研究显示肿瘤内的干细胞样细胞对手术及放化疗均耐受。恶性脑肿瘤中，肿瘤内的干细胞样细胞在肿瘤的复发、血管生成及侵袭过程中均发挥了重要作用。然而，脑膜瘤内的干细胞样细胞尚未被鉴定出来。本研究中，我们鉴定出了人脑膜瘤内具有成球能力的干细胞样细胞。结果显示，脑膜瘤内的肿瘤干细胞样细胞具备在干细胞特定培养条件下形成细胞球的能力。这些脑膜瘤细胞球（MgSCs）在免疫荧光染色时表达祖细胞的标志物CD133，但不表达分化细胞的标志物：上皮膜抗原（EMA）。重要的是，MgSCs细胞的ABCG及CD133的mRNA表达明显高于贴壁的脑膜瘤细胞（MgACs）。同时，MgSCs细胞对化疗药长春新碱的耐受性也强于MgACs细胞。由细胞球分离出的单个MgSCs细胞也较MgACs细胞具有更强的耐药性。而且，MgSCs细胞对辐射的耐受性也较MgACs细胞更强。此外，MgSCs细胞在免疫缺陷小鼠体内进行原位接种时，也表现出更强的成瘤性。其形成的肿瘤进行相应的免疫组化染色，移植瘤的MgSCs与人体内的原发肿瘤相一致，同样表达EMA、波形蛋白及CD133。这些发现使人更好地认识了脑膜瘤细胞的生物学特性，针对脑膜瘤内干细胞样细胞的治疗也有望成为耐药脑膜瘤治疗新的作用点。
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3.原文：Recent advances in research have found that tumor stem-like cells are resistant to surgery, radiotherapy, and chemotherapy. Tumor stem-like cells play critical roles+ c e6 D0 _1 `* K: }
in tumor recurrence, angiogenesis, and invasion in malignant brain tumors. However, the identification of tumor stem-like cells in meningiomas has not been clarified. In this study, we identified the stem-like features of sphereforming cells in human meningiomas. The results showed that meningioma stem-like cells possess the ability to form- N" g z. a0 x$ ^3 L7 X
spheres in identical stem cell culture condition. These meningioma sphere cells (MgSCs) expressed progenitor cell marker, CD133, but not differentiated cell marker, epithelial membrane antigen (EMA) on immunofluorescence staining. Importantly, the mRNA expression of ABCG, and CD133 was higher in MgSCs than daughter meningioma adherent cells (MgACs). In addition, cells displayed chemotherapeutic resistance to vincristine more in MgSCs than MgACs. This phenomenon was also found in single cell form from dissociated spheres than MgACs. Moreover, MgSCs are more resistant to irradiation treatment than MgACs. Furthermore, MgSCs revealed high tumorigenicity in vivo following orthotopic inoculation into the brains of immunodeficient mice. The corresponding immunohistochemical (IHC) staining found that MgSCs are positive for EMA, vimentin, and CD133, consistent with IHC of primary human meningiomas. These findings have provided better understanding of meningioma cell biology and suggested that meningioma stem-like cells may serve as a novel target in therapeutic resistant meningiomas.; Y( }) b( i( `, M p# ^
1 k/ Q" k) N" @( \6 P4.原文附件如下4 U, t+ u, Y9 M5 C# n: ~7 {
" J, R! L# \( Z. e8 J5.个人翻译，理解不准确的语句还望大家积极指出。
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6.个人认为这篇文章并没有多少新奇之处，依然是在肿瘤干细胞研究的那个套路里打转转，多数鉴定肿瘤干细胞的研究都为这一思路（标志物+悬浮培养+耐药+接种成瘤性），肿瘤干细胞的分离鉴定亟需有方法学上的突破。翻译这个的目的是想大家有没有什么好的想法，能跳出这个圈子，另辟蹊径，进行肿瘤干细胞的研究。欢迎讨论指正！
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发表于 2010-12-5 00:26
发表于 2010-12-5 08:01
发表于 2010-12-5 10:57
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