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本帖最后由 细胞海洋 于 2010-12-5 20:08 编辑 1 H+ g0 P& ]6 T% Q& ] _- c# K, W
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说明：原文来源自cell stem cell由干细胞之家新闻小组成员sun_happone翻译（转帖请注明）"
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翻译内容: 诱导多能干细胞iPS技术，也就是通过加入特定的转录因子使体细胞重编程为多能性细胞，这些多能性细胞类似于胚胎干细胞，这在生物医学研究和再生医学中具有巨大的应用潜能。现在，科学家们正在应用各种技术，在没有外源基因的诱导下产生iPS，这再次成为iPS应用的一个主要障碍。为实现只用一个特定的小分子获得iPS的最终目标，科学家们正进行不断的努力来鉴定各种化合物，使其在功能上代替外源性的重编程TFs或增强重编程效率和动力学。到目前为止，只有神经干细胞表达高水平的SOX2和C-MYC,通过外在的表达OCT4，能够重编程为iPS。然而，胎儿的神经干细胞很少也很难获得。因此，为其他更易获得的体细胞寻找重编程的条件显得尤为重要。在此，我们介绍一种小分子物质，它只外源性表达OCT4，能使人主要的体细胞重编程为iPS。
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Reprogramming of Human Primary Somatic Cells by OCT4 and Chemical Compounds
. ]' k$ f5 `/ ~+ r7 C9 t( o8 Z1 R: N原文：Induced pluripotent stem cell (iPSC) technology, i.e. reprogramming somatic cellsinto pluripotent cells that closely resemble embryonic stem cells (ESCs) by introduction of defined transcription factors (TFs), holds great potential in biomedical research and regenerative medicine。Various strategies have been developed to generate iPSCs with fewer or no exogenous genetic manipulations, which represent a major hurdle for iPSC applications.With the ultimate goal of generating iPSCs with a defined small molecule cocktail alone, substantial effort and progress have been made in identifying chemical compounds that can functionally replace exogenous reprogramming TFs and/or enhance the efficiency and kinetics of reprogramming。To date, only neural stem cells (NSCs), which endogenously express SOX2 and cMYC at a high level, have been reprogrammed to iPSCs by exogenous expression of just OCT4.However, human fetal NSCs are rare and difficult to obtain. It is therefore important to develop reprogramming conditions for other more accessible somatic cells. Here we report a small molecule cocktail that enables reprogramming of human primary somatic cells to iPSCs with exogenous expression of only OCT4.
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4 Z2 n/ U2 }$ f4 _个人翻译，理解不准确的语句还望大家积极指出; r# [% h* G* `5 [/ }0 f5 C
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发表于 2010-12-5 19:25
