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本帖最后由 细胞海洋 于 2011-1-6 19:19 编辑 ( Z* y/ n; _! F9 i& K/ q
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Oct4-EGFP transgenic pigs - a new large animal model for reprogramming studies.
! s* A, l9 Z5 }# u6 H原文来源自http://www.ncbi.nlm.nih.gov/pubmed
# t. u) \9 D8 g8 x S 由干细胞之家新闻小组成员hyde翻译（转帖请注明）
) y! P5 @$ Z' ?! |; |1 R2 a+ INowak-Imialek M, Kues WA, Petersen B, Lucas-Hahn A, Herrmann D, Meena Haridoss S, Oropeza M, Lemme E, Schöler HR, Carnwath JW, Niemann H.
. k) H* q9 X* C5 x0 I+ o+ E: sInstitute of Farm Animal Genetics (FLI), Biotechnology, 31535 Neustadt, Germany; Monika.Nowak-Imialek@fli.bund.de.! \" @. ^: }/ W9 Y u
- C- i" u' E; Z" {摘要：+ P5 T U R% F
舍养驯化的猪逐渐成为了一种重要的工具用于：外科技术的发展、异种移植的发展、重要疾病模型的建立、新型细胞治疗技术的临床测试等。但是猪生殖系细胞来源的多能性猪细胞没有被建立。这一直是猪基因改造的主要障碍。转录因子Oct4对于多能性的维持和体细胞重新编程为多能状态是必不可少的。我们报道的生产出来的转基因猪携带了一个18kb的鼠Oct4基因，该基因与增强型绿色荧光蛋白的CDNA结合使用绿色荧光蛋白监控ot4的表达来识别多能性细胞。通过体细胞核移植获得了十一头可行的转基因仔猪，EGFP在以下部位表达：生殖细胞系、ICM、滋养层囊胚、睾丸生殖细胞。通过将这些动物中的成纤维细胞与小鼠胚胎多功能干细胞融合重编程或病毒转导人类 OCT4, SOX2, KLF4 和c-MYC cDNAs重编程，激活 Oct4-EGFP的表达。这个OG2猪被证明能有效地监测重编程、诱导和维持猪多能细胞。总而言之，OG2转基因猪是一个新的大动物模型，用于研究诱导和维持多能性细胞，在发展细胞性治疗方面也有重要的意义。
* H [# }$ I# b! OAbstract
2 G P) u: [, ]The domesticated pig has emerged as an important tool for development of surgical techniques, advancement of xenotransplantation, creation of important disease models and preclinical testing of novel cell therapies. However, germ line competent pluripotent porcine stem cells have not yet been derived. This has been a major obstacle to genetic modification of pigs. The transcription factor Oct4 is essential for the maintenance of pluripotency and for reprogramming somatic cells to a pluripotent state. Here, we report the production of transgenic pigs carrying an 18 kb genomic sequence of the murine Oct4 gene fused to the enhanced green fluorescent protein (EGFP) cDNA (OG2 construct) to allow identification of pluripotent cells by monitoring Oct4 expression by EGFP fluorescence. Eleven viable transgenic piglets were produced by somatic cell nuclear transfer. Expression of the EGFP reporter construct was confined to germ line cells, the inner cell mass and trophectoderm of blastocysts, and testicular germ cells. Reprogramming of fibroblasts from these animals by fusion with pluripotent murine embryonic stem cells or viral transduction with human OCT4, SOX2, KLF4 and c-MYC cDNAs resulted in Oct4-EGFP reactivation. The OG2 pigs have thus proved useful for monitoring reprogramming and the induction and maintenance of pluripotency in porcine cells. In conclusion, the OG2 transgenic pigs are a new large animal model in which to study the derivation and maintenance of pluripotent cells, and will be valuable for the development of cell therapy.
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, ^5 S4 {( C- B# T) `6 ]: a个人翻译，理解不准确的语句还望大家积极指出。* m9 ^& o, e/ N
" O/ C0 g4 P9 v" {海洋注：5楼原文 感谢songzxj 提供 |
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发表于 2010-12-8 19:17
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