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探索人羊水干细胞的干性和在神经方面的作用4 T! j! H% y7 P6 T) w
说明：原文来源自cell stem rev and rep由干细胞之家新闻小组成员sun_happone翻译（转帖请注明）"
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3 h/ ?. N1 k' q& b( p. G& H" R( P翻译内容: 近日，人的羊水干细胞作为一个可供选择的细胞类型应用于移植和组织工程而受到极大的关注。羊水细胞包含来源于胎儿组织的各种细胞类型，据说其中有一小部分代表干细胞的下一层细胞，与人的胚胎干细胞相比，羊水细胞不易受到法律和伦理的考虑，也不会受到成体干细胞系的限制。然而，为了使其具有医疗价值，应该有一种更好的方法，能把羊水干细胞分离成下一层细胞，此项研究旨在分析各个不同孕期的羊水细胞在自我更新、分化等方面的分子组成。我们的结果显示，羊水细胞虽然在形态学上具有异质性，但来源于早期妊娠期的羊水细胞无所不在地表达K8，表明大部分的羊水细胞是上皮来源的。除此之外，羊水细胞表达NOTCH信号的各种成分，这是涉及干细胞维持、决定、分化的信号通路。K8阳性细胞的下一层细胞共表达NESTIN，这个标记物在神经上皮、神经干细胞和神经前体细胞中被发现，在整个妊娠期，小于1%的羊水细胞表达多能性标记物OCT4a,NANOG,SOX2，其中SOX2+ 羊水细胞可以通过单个细胞克隆来分离，表达羊水细胞克隆的SOX2表明他具有形成神经表型的能力，表达神经的标记物MAP2,NFL,NSE，把这些因素都考虑进去，我们的发现表明在羊水中存在具有干细胞特征的胎儿细胞，表明羊水细胞在生物学和临床应用等方面的作用。
6 \ b6 ?, i) j2 F' a原文：Recently, human amniotic fluid (AF) cells have attracted a great deal of attention as an alternative cell source for transplantation and tissue engineering. AF contains a variety of cell types derived from fetal tissues, of which a small percentage is believed to represent stemcell sub-population(s). In contrast to human embryonic9 a+ U( [( A6 t- |7 N% g+ U* j
stem (ES) cells, AF cells are not subject to extensive legal or ethical considerations; nor are they limited by lineage commitment characteristic of adult stem cells. However, to become therapeutically valuable, better protocols for the isolation of AF stem cell sub-populations need to be developed. This study was designed to examine the molecular components involved in self-renewal, neural commitment and differentiation of AF cells obtained at different gestational ages. Our results showed that, although morphologically heterogeneous, AF cells derived from early gestational periods ubiquitously expressed KERATIN 8 (K8), suggesting that the majority of these cells may have an epithelial origin. In addition, AF cells expressed various components of NOTCH signaling (ligands, receptors and target genes), a pathway involved in stem cell maintenance, determination and differentiation. A sub-population of K8 positive cells (<10%) co-expressed NESTIN, a marker detected in the neuroepithelium, neural stem cells and neural progenitors. Throughout the gestational periods, a much smaller AF cell sub-population (<1%) expressed pluripotency markers, OCT4a, NANOG and SOX2, from which SOX2 positive AF cells could be isolated through single cell cloning. The SOX2 expressing AF clones showed the capacity to give rise to a neuron-like phenotype in culture, expressing neuronal markers such as MAP2, NFL and NSE. Taken together, our findings demonstrated the presence of fetal cells with stem cell characteristics in the amniotic fluid, highlighting the need for further research on their biology and clinical applications.
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原文附件如下：
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' D* y1 K1 ^. B+ I2 y: S+ H个人翻译，理解不准确的语句还望大家积极指出
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发表于 2010-12-12 23:15
