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分离羊水干细胞系的潜能用于治疗
- m9 Q3 ^! s$ _* I! c说明：原文来源自nature biotechnology 由干细胞之家新闻小组成员sun_happone翻译（转帖请注明）"+ U: k9 b: k3 K+ K# \
干细胞具有分化为各种细胞系的能力，对于治疗来说，可能是有价值的。我们曾报道过分离人和啮齿动物的羊水干细胞（AFS）—来源于干细胞—可表达胚胎和成体干细胞的标记物。未分化的AFS细胞可以在没有滋养层的条件下广泛的扩增，在36小时就可以扩增1倍，并且不会形成肿瘤。AFS细胞具有广泛的多能性，通过病毒感染法标记克隆人的细胞系能够诱导分化形成代表每个胚层的各种细胞类型，包括生脂细胞、成骨细胞、肌原细胞、内皮细胞、神经细胞、肝细胞。来源于人的羊水干细胞的已分化细胞表现特殊的功能，包括神经元细胞分泌的神经递质L-谷氨酸盐或向内表达G蛋白通道修正钾通道，肝细胞系产生尿素，成骨细胞形成组织工程骨骼。: d2 Y" q: v# H1 r( x$ V' U& \. q
原文：Stem cells capable of differentiating to multiple lineages may be valuable for therapy. We report the isolation of human and rodent amniotic fluid–derived stem (AFS) cells that express embryonic and adult stem cell markers. Undifferentiated AFS cells expand extensively without feeders, double in 36 h and are not tumorigenic. Lines maintained for over 250 population doublings retained long telomeres and a normal karyotype. AFS cells are broadly multipotent. Clonal human lines verified by retroviral marking were induced to differentiate into cell types representing each embryonic germ layer, including cells of adipogenic, osteogenic, myogenic endothelial, neuronal and hepatic lineages. Examples of differentiated cells derived from human AFS cells and displaying specialized functions include neuronal lineage cells secreting the neurotransmitter L-glutamate or expressing G-protein-gated inwardly rectifying potassium channels, hepatic lineage cells producing urea, and osteogenic lineage cells forming tissue-engineered bone.
) ]! h7 `! v$ c( b2 O原文附件如下：
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个人翻译，理解不准确的语句还望大家积极指出8 h- t0 O; _5 [, x& d) F
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发表于 2010-12-17 19:57
