本帖最后由 细胞海洋 于 2010-12-31 20:33 编辑
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美国威斯康辛大学麦迪逊分校的科学家们近日开发出一套新细胞培养系统，有望为细胞治疗提供更加一致和安全的细胞培养物。11月14日的《自然—方法学》刊登了这一成果。
1 @9 ?. C% R" A7 U4 R5 `1 N领导这项研究的Laura Kiessling教授表示这套系统并不昂贵，可承担多能细胞培养中的大量预估工作。这项技术“很简单”，“任何人都能使用”。
) ~0 o, ~6 ^" b9 a. w目前，大部分人类胚胎干细胞的培养都是作为实验室研究用途的，而通常所采用的培养方法又容易造成各种污染。6 ?2 _# |. ?& ]; ~+ E! d* B
这套新系统采用一种化学合成制得的可与干细胞相结合的蛋白片段底物，并通过与特定培养基结合，可将细胞培养固定在未分化阶段达三个月或者更长时间。据报告显示，该系统亦可用于诱导多功能干细胞培养。
% s8 [- Z% f' m, n7 X“科学家现在普遍采用的培养系统都有着非特定的缺点，并且缺乏一致性，以致出现质量差异”， 领导这项研究的Laura Kiessling教授解释，“我们研发的这套系统特定性很好，而且并不贵”。（科学网 张笑/编译）
0 G! e" Z+ E K y; {相关仪器及方法：新型细胞培养系统 IX81显微镜 7500 Fast实时PCR系统 发光光度计& W4 B& S( t( }
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A defined glycosaminoglycan-binding substratum for human pluripotent stem cells
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Joseph R Klim,Lingyin Li,Paul J Wrighton,Marian S Piekarczyk& Laura L Kiessling
* L- u8 b/ s" y! [' wAffiliationsContributionsCorresponding author Journal name:Nature Methods9 I. V+ A4 z+ v6 Y! L. B
Volume:7,Pages:989–994 Year published 2010)DOI:doi:10.1038/nmeth.1532 Received10 March 2010Accepted21 October 2010Published online14 November 2010* T$ i4 y A: G# Q' B# s1 k1 L$ L
Abstract
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ConnoteaCite U LikeFacebookTwitterDeliciousDiggTo exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. Media of known composition that maintain human embryonic stem (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficult. We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. The effective substrates displayed heparin-binding peptides, which can interact with cell-surface glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 months. The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. The peptides supported growth of eight pluripotent cell lines on a variety of scaffolds. Our results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells.
3 V. Y* ~" X# {( k# rhttp://www.nature.com/nmeth/journal/v7/n12/abs/nmeth.1532.html
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. o' ]2 U5 B m7 K- A5楼原文 感谢glysh 提供 | 
发表于 2010-12-30 18:58
发表于 2010-12-30 19:09
