线粒体功能控制胚胎干细胞增殖和早期分化潜能 - 干细胞行业新闻干细胞之家



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发表于 2011-1-5 16:25 |只看该作者 |倒序浏览 |打印

Mitochondrial Function Controls Proliferation and Early Differentiation Potential of Embryonic Stem Cells3 j. f# A! q! m: f- q& f: Y

说明：原文来源自http://onlinelibrary.wiley.com/doi/10.1002/stem.590/pdf
由干细胞之家新闻小组成员Hyde翻译（转帖请注明）
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摘要：多能干细胞因为具有无限的自我更新能力和分化潜力，是再生医学的重要研究对象。最近我们的研究发现适当的线粒体功能对于未分化ESC的再生有重要的作用。当线粒体功能衰减时，自我更新的ESC更多的依赖糖酵解，同时伴随着Nanog，Oct4，Sox2相关mRNA增加积累。相比之下，在分化过程前7天中的线粒体功能衰减细胞表达正常的 Oct4, Nanog and Sox2。但是分化的效果大打折扣，大量转录multiple Hox genes。此外，在线粒体功能衰减的分化过程中，瘤细胞一直存在。我们的实验结果表明了在ESC自我更新、调节分化、避免癌化中建立正常的线粒体功能的重要性。! A' P6 }7 `! v w+ p

ABSTRACT* \% C9 }5 b5 i) c
Pluripotent stem cells hold significant promise in regenerative medicine due to their unlimited capacity for self-renewal, and potential to differentiate into any cell type of the body. In the current study, we demonstrate that proper mitochondrial function is essential for proliferation of undifferentiated embryonic stem cells (ESCs). Attenuating mitochondrial function under self-renewing conditions
makes these cells more glycolytic-dependent, and is associated with an increase in the mRNA reserves of Nanog, Oct4 and Sox2. In contrast, attenuating mitochondrial function during the first 7 days of differentiation results in normal repression of Oct4, Nanog and Sox2. However differentiation potential is compromised as revealed by abnormal transcription of multiple Hox genes. Furthermore, under differentiating conditions in which mitochondrial function is attenuated, tumorigenic cells continue to persist. Our results, therefore establish the importance of normal mitochondrial function in ESC proliferation, regulating differentiation, and preventing the
emergence of tumorigenic cells during the process of differentiation.

注明：个人翻译，理解不准确的语句还望大家积极指出。

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