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1. 主题:核接头蛋白LDB1通过调节转录来调控和维持造血干细胞稳定性/ [; G7 h; h5 h6 v
2. 原文主题: Nuclear adaptor Ldb1 regulates a transcriptional program essential for the maintenance of hematopoietic stem cell" J p# H* I* V9 T
3. 摘要翻译: 细胞核接头蛋白LDB1是多蛋白转录复合体中的一个核心成分,在细胞的分化过程起到调节作用.在造血系统发育中,ldb1同非DNA结合的接头蛋白Lmo2,转录因子E2A,scl和Gata1 (或者Gata 2) 形成一个复合体.在这篇文章中,作者发现在胚胎和成体的小鼠造血干细胞中, LDB1起到很重要的作用. 在造血干细胞和前体细胞中敲除该基因会造成一些和干细胞维持有关的许多转录本的下调.全基因组的免疫共沉淀-测序研究显示LDB1形成的复合体结合在这些调节干细胞维持相关的基因的启动子区域. 通过这项研究,作者认为Ldb1在调节转录,进而维持干细胞的状态中起到核心的作用.
0 _9 W( J5 p2 U: u' k. W, M4. 原文摘要: The nuclear adaptor Ldb1 functions as a core component of multiprotein transcription complexes that regulate differentiation in diverse cell types. In the hematopoietic lineage, Ldb1 forms a complex with the non–DNA-binding adaptor Lmo2 and the transcription factors E2A, Scl and GATA-1 (or GATA-2). Here we demonstrate a critical and continuous requirement for Ldb1 in the maintenance of both fetal and adult mouse hematopoietic stem cells (HSCs). Deletion of Ldb1 in hematopoietic progenitors resulted in the downregulation of many transcripts required for HSC maintenance. Genome-wide profiling by chromatin immunoprecipitation followed by sequencing (ChIP-Seq) identified Ldb1 complex–binding sites at highly conserved regions in the promoters of genes involved in HSC maintenance. Our results identify a central role for Ldb1 in regulating the transcriptional program responsible for the maintenance of HSCs.
4 }7 W0 [7 p r, S' ?- d' q% O5. 原文链接http://www.nature.com/ni/journal/vaop/ncurrent/full/ni.1978.htm
. K$ v. [! o+ `8 N! R6. 个人观点:首先这篇研究是一个经典HSC的研究,转录(调节)因子,然后array发现一些相关的gene都被调控了,而且用in vitro的方法显示这些因子的启动子或者enhancer等区域该gene 产物的结合. 但是,这样的co-relation的研究太多,我个人觉得应该是JEM级别的paper.不过还是恭喜本家!
6 k6 T [- A4 ^' t7. 个人翻译,如有不当,请指出.* L- S9 d) g/ l7 k! p2 _ L
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发表于 2011-1-5 21:14
