|
 
- 积分
- 981
- 威望
- 981
- 包包
- 1968
|
本帖最后由 细胞海洋 于 2011-1-9 10:16 编辑
, |4 j, F* ^. P0 ~& V$ L& |' Y( k0 H: O
1.说明：原文来源自http://www.plosone.org/article/i ... e.0014414#abstract0 由干细胞之家新闻小组成员不倒翁翻译（转帖请注明）
: |- r0 N1 j, u; {2 Q/ B" _1 r: [( T2.翻译内容
6 |3 ], Y# R) t1 t! L纤维母细胞生长因子-10促进胚胎干细胞和诱导多能干细胞向心肌细胞的分化' y3 b* D4 S/ t* j1 U( i; N
摘要. R% ^1 y( g9 I$ m3 Z
背景: b, u9 h" b( e" q0 o
纤维母细胞生长因子（FGF）家族是正常心脏形成必须的。然而，其在干细胞向心肌细胞的分化中的贡献还没经系统的研究 。本研究中，我们检测了FGF家族蛋白处理的胚胎干细胞和诱导多能干细胞向心肌细胞分化过程中的机制和作用。
" ^7 I& R1 B! H方法和结果5 [8 K P% Z/ v* @
我们用鼠的ES细胞和ips细胞稳定转染心脏特异的α-肌浆球蛋白重链启动子控制的增强型绿色荧光蛋白（eGFP）来监测心肌细胞的分化。在鼠的ES向心肌细胞分化的过程中，FGF-3, -8, -10, -11, -13和 -15的表达模式跟中胚层特异的标记基因短尾肽（Brachyury）和心肌细胞前体的标记基因胎肝激酶-1（Flk-1）的表达模式相似。在他们当中，FGF-10诱导心肌细胞分化呈现时间和浓度依赖模式。FGF-10中和抗体，小分子的FGF受体拮抗剂PD173074和FGF-10以及FGF受体-2的shRNA抑制心肌细胞分化。FGF-10同样促进鼠的ips细胞分化成心肌细胞系，这种促进作用被FGF-10的中和抗体或PD173074抵消。根据基因本体分析，基因芯片数据显示心脏发育相关基因在FGF-10处理后上调。在体内，心肌内共同加入FGF-10和ES细胞显示FGF-10同样能促进心肌细胞的分化。+ w8 F# y. C3 c9 G
结论及意义5 y) L0 z% _+ z
FGF-10诱导ES细胞和ips细胞分化为心肌细胞，可能具有临床应用的前景。
9 E( S. @7 E$ \) ]5 c' M
9 s) Q q% x' C9 K$ ~0 Z3.原文- |4 u* C5 g0 Q# U( P& h# q
Fibroblast Growth Factor-10 Promotes Cardiomyocyte Differentiation from Embryonic and Induced Pluripotent Stem Cells. g( \ Y: ]" a; o& o- w8 A5 X8 O
7 j1 e2 J1 l+ r+ s- cAbstract
1 v% I% r& p2 ]& O& S( u8 f L( NBackground
0 [% v# A# h( s2 l5 |The fibroblast growth factor (FGF) family is essential to normal heart development. Yet, its contribution to cardiomyocyte differentiation from stem cells has not been systemically studied. In this study, we examined the mechanisms and characters of cardiomyocyte differentiation from FGF family protein treated embryonic stem (ES) cells and induced pluripotent stem (iPS) cells.
1 s3 s* k1 w9 H4 z% W" k5 JMethodology/Principal Findings
+ y: e W9 a( i% n9 W4 C* EWe used mouse ES cells stably transfected with a cardiac-specific α-myosin heavy chain (αMHC) promoter-driven enhanced green fluorescent protein (EGFP) and mouse iPS cells to investigate cardiomyocyte differentiation. During cardiomyocyte differentiation from mouse ES cells, FGF-3, -8, -10, -11, -13 and -15 showed an expression pattern similar to the mesodermal marker Brachyury and the cardiovascular progenitor marker Flk-1. Among them, FGF-10 induced cardiomyocyte differentiation in a time- and concentration-dependent $ A: e5 T4 c! N1 {: o- u5 b$ Z
manner. FGF-10 neutralizing antibody, small molecule FGF receptor antagonist PD173074 and FGF-10 and FGF receptor-2 short hairpin RNAs inhibited cardiomyocyte differentiation. FGF-10 also increased mouse iPS cell differentiation into cardiomyocyte lineage, and this effect was abolished by FGF-10 neutralizing antibody or PD173074. Following Gene Ontology analysis, microarray data indicated that genes involved in cardiac development were upregulated after FGF-10 treatment. In vivo, intramyocardial co-administration of FGF-10 and ES cells demonstrated that FGF-10 also promoted cardiomyocyte differentiation.- I1 R1 f3 Y3 l5 R
Conclusion/Significance. `+ [+ V) c$ Q z# n6 h
FGF-10 induced cardiomyocyte differentiation from ES cells and iPS cells, which may have potential for translation into clinical applications.7 v$ @/ P& z, ~ u5 P& b5 b/ j
/ w d# g) Z3 }9 N- H" K( @; {
4.如果是文献可以上传原文附件或者下载连接
! w7 f5 v" h& F. c6 x3 ihttp://www.plosone.org/article/i ... e.0014414#abstract0, A6 E+ p, j2 y
5..注明：个人翻译，理解不准确的语句还望大家积极指出。( f4 V! M6 j- L4 V0 ~4 y2 K& _
' S/ j) ^/ y" K7 ?8 F+ A+ w0 i0 \
海洋注：4楼原文 感谢小鲁鲁 提供 |
-
总评分: 威望 + 20
包包 + 100
查看全部评分
|
发表于 2011-1-6 16:10
