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5 [1 ~; v* U$ F4 UTargeted integration in rat and mouse embryos with zinc-finger nucleases
3 t( { l: y- w$ t' z使用锌指核酸对鼠（大、小）胚胎进行基因的定点整合
$ F& N4 D& R+ P2 r7 x4 j4 s1.说明：原文来源自http://www.nature.com/nbt/journa ... /full/nbt.1731.html& W9 v7 q( a! ^; Q4 D$ u
由干细胞之家新闻小组成员Hyde翻译（转帖请注明）
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Xiaoxia Cui,Diana Ji,Daniel A Fisher,Yumei Wu,David M Briner& Edward J Weinstein- v H$ Y3 f P! e
Affiliations Contributions Corresponding author Journal name: 7 c' f& O; j; M* B1 Y
Nature Biotechnology $ D0 Y& a5 ?8 u- N$ a n/ ]
Year published:
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& r7 m) b0 O2 `) }7 q' }2 pdoi:10.1038/nbt.1731 4 U0 r: e' e0 X* }
Received 09 April 2010 Accepted 10 November 2010 Published online 12 December 2010 ; ]) h) N: q1 L/ d6 A$ C+ N' ^2 N
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2.翻译内容! i% d8 S0 \4 J6 o1 c- y, N0 J
摘要：基因打靶技术是反向遗传学和疾病动物模型建立中不可或缺的。小鼠已成为最常用的动物模型系统，因为以其胚胎干细胞为基础的基因定位技术比较成熟，而其他哺乳动物缺乏便捷的修饰基因组的工具。最近有研究者使用显微注射技术将修饰的锌指核酸酶（ZFNs）注入胚胎，产生基因敲除的小鼠和大鼠，引入非同源末端连接介导的缺失或在目标点的插入（？）。我们使用ZFN技术引入序列特异性的修饰（knock-ins）。实验中在Sprague Dawley 、 Long-Evans hooded rats、FVB mice中进行同源重组。这种方法可以进行精确的基因工程修改，如点突变、准确的插入和缺失以及特定的基因敲除和敲入。同样的策略将来可能会被应用在其他物种的基因工程改造中。2 Q: R4 m4 ]. E3 Y* |5 K2 H
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3.原文) d7 z/ G7 Z" [! N( W# J7 g
摘要：Gene targeting is indispensible for reverse genetics and the generation of animal models of disease. The mouse has become the most commonly used animal model system owing to the success of embryonic stem cell–based targeting technology, whereas other mammalian species lack convenient tools for genome modification. Recently, microinjection of engineered zinc-finger nucleases (ZFNs) in embryos was used to generate gene knockouts in the rat and the mouse by introducing nonhomologous end joining (NHEJ)-mediated deletions or insertions at the target site. Here we use ZFN technology in embryos to introduce sequence-specific modifications (knock-ins) by means of homologous recombination in Sprague Dawley and Long-Evans hooded rats and FVB mice. This approach enables precise genome engineering to generate modifications such as point mutations, accurate insertions and deletions, and conditional knockouts and knock-ins. The same strategy can potentially be applied to many other species for which genetic engineering tools are needed.
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P3 O" \- E( l: q! g% D4 S) u$ p4.原文附件* O' ]& x5 k, o) e
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5.注明：个人翻译，理解不准确的语句还望大家积极指出。
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U; w( k0 p" N4 x, p. B感觉像一种广告……方法还不错……分享一下 |
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发表于 2011-1-10 16:11
