鉴定和分离来源于成体骨髓或G-CSF活化到外周血液的CD34+/CD133+/CXCR4+/ Lin-CD45-… - 干细胞行业新闻干细胞之家



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hyde

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发表于 2011-1-20 12:50 |只看该作者 |倒序浏览 |打印

Identification and isolation from either adult human bone marrow or G-CSF mobilized peripheral blood of CD34+/CD133+/CXCR4+/ Lin-CD45- cells, featuring morphological, molecular and phenotypic characteristics of very small embryonic-like (VSEL) stem cells

1.说明：原文来源自/ I; X5 G3 y- O/ S
http://www.sciencedirect.com/sci ... 6f&searchtype=a# W7 S( A2 X* C1 O7 i8 m( B
由干细胞之家新闻小组成员hyde翻译（转帖请注明）

Hanna Sovalata, Maurice Scrofania, Antoinette Eidenschenka, Stéphanie Pasqueta, Valérie Rimelenb and Philippe Hénona# Y7 [7 s( K: ?
a Institut de Recherche en Hématologie et Transplantation; Mulhouse, France! @$ Z# k9 ~* f; {  g. L
b Département d'Hématology, Hôpital E. Muller; Mulhouse, France* T1 B6 o7 O8 d, u* E
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Received 27 October 2010;  revised 28 December 2010;  accepted 10 January 2011.  Available online 14 January 2011.
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2.翻译内容- A; q% M- c9 \; L$ O) F

摘要：
Abstract2 b0 U" r4 N% Q& L' G& h5 ~
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目标：
最近我们发现正常的人类骨髓中分化的CD34+细胞（在粒细胞集落刺激因子的活化作用下进入外周血液）存在一些细胞亚群参与内皮细胞和心肌分化途径。这些细胞亚群能够在心内自由转运，对于缺血性心肌损伤后的再生具有重要的意义。我们推断这些相关的细胞亚群来源于非常小的胚胎样干细胞(VSELs).这些VSELs在个体发育时存在于骨髓中，并在储存在成体的骨髓中直到生命周期的结束，在粒细胞集落刺激因子的活化下这些细胞进入外周血液循环系统。* q7 l# `6 N5 w8 r
Objective
Recently, we demonstrated that normal human BM (hBM)-derived CD34+ cells, released into the peripheral blood (PB) after granulocyte colony-stimulating factor (G-CSF) mobilization, contain cell subpopulations committed along endothelial and cardiac differentiation pathways. These subpopulations could play a key role in the regeneration of post-ischemic myocardial lesion after their direct intracardiac delivery. We hypothesized that these relevant cells might be issued from very small embryonic-like stem cells (VSELs) deposited in the BM during ontogenesis and resident all life long in the adult BM and that they could be mobilized into PB by G-CSF." a6 |3 V& P* w2 A6 D: u
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材料和方法：
采集正常人类骨髓和白细胞除去法获得的骨髓（来源于癌症病人，进行G-CSF 活化的分析和通过多参数流式细胞术进行分类）（这个步骤不太明白？）。用免疫荧光和RT-qPCR分析典型的多能干细胞marker的表达情况。+ F3 W, d1 K" m$ q" J% `& Z5 m
Materials and Methods
Samples of normal hBM and leukapheresis products (LKP) harvested from cancer patients after G-CSF mobilization were analyzed and sorted by multiparameter flow cytometry (FCM) strategy. Immunofluorescence and reverse transcription-quantitative-polymerase chain reaction (RT-qPCR) assays were performed to analyse the expression of typical pluripotent stem cells (PSC) markers.
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结果：
从hBM 或from LKP分离的到一群f CD34+/CD133+/CXCR4+/Lin- CD45-不成熟的细胞。在这些细胞群体中，少数细胞(2 to 5μm)被检查到在蛋白或者基因水平表达 Oct-4, Nanog and stage specific embryonic antigen-4 (SSEA-4) 。
Results
A population of CD34+/CD133+/CXCR4+/Lin- CD45- immature cells was first isolated from the hBM or from LKP. Among this population, very small (2 to 5μm) cells expressing Oct-4, Nanog and stage specific embryonic antigen-4 (SSEA-4) at protein and mRNA levels were identified.

结论：: \. r/ \( t# ]# b3 }% M
我们的研究支持了假设，即存在VSELs 构成了一个可移动的 primitive/pluripotent stem cells 来源，它们在多种因素（生理或病理
的刺激）的作用下从骨髓中被释放进入外周血液。
Conclusions
Our study thus supports the hypothesis that VSELs constitute a “mobile” pool of primitive/pluripotent stem cells that could be released from the BM into the PB under the influence of various physiological or pathological stimuli./ h8 r  c+ C' C, c

Key Words: Pluripotent stem cells; very small embryonic-like stem cells; leukapheresis product; human bone marrow; CD34+/CD133+/CXCR4+cells; Oct-4; stage-specific embryonic antigen-4
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注明：个人翻译，理解不准确的语句还望大家积极指出。5 K8 Q& j4 z) v
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tpwang

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发表于 2011-1-20 13:56 |只看该作者

回复 hyde 的帖子

翻的不错，几点建议。翻过来的汉语句型不必紧扣原文，可以适当调整，比如从句可以直接翻译进主句，带从句的主句可以分开翻译等。举例而言：+ d' X x' @4 K
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Recently, we demonstrated that normal human BM (hBM)-derived CD34+ cells, released into the peripheral blood (PB) after granulocyte colony-stimulating factor (G-CSF) mobilization, contain cell subpopulations committed along endothelial and cardiac differentiation pathways.
最近我们发现正常的人类骨髓中分化的CD34+细胞（在粒细胞集落刺激因子的活化作用下进入外周血液）存在一些细胞亚群参与内皮细胞和心肌分化途径。

我们最近的研究表明，粒细胞集落刺激因子可以动员正常人骨髓来源的CD34+细胞进入外周血，而且这些细胞包含定向分化为内皮和心脏细胞的亚群。+ C2 E2 t4 N. R' d3 g
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These subpopulations could play a key role in the regeneration of post-ischemic myocardial lesion after their direct intracardiac delivery. ) W4 o7 U# I. c3 o
这些细胞亚群能够在心内自由转运，对于缺血性心肌损伤后的再生具有重要的意义。' b5 I- p& W$ Q7 S* t

通过直接心内输注，这些细胞亚群有可能在缺血性心肌损伤的再生中起关键的作用。

We hypothesized that these relevant cells might be issued from very small embryonic-like stem cells (VSELs) deposited in the BM during ontogenesis and resident all life long in the adult BM and that they could be mobilized into PB by G-CSF.
我们推断这些相关的细胞亚群来源于非常小的胚胎样干细胞(VSELs).这些VSELs在个体发育时存在于骨髓中，并在储存在成体的骨髓中直到生命周期的结束，在粒细胞集落刺激因子的活化下这些细胞进入外周血液循环系统。

我们推断，这些亚群有可能来源于极小胚胎样干细胞（VSELs），这些干细胞通过发育过程落户在骨髓中并终身存在，而且有可能通过G-CFG动员这些细胞进入外周血循环。, K2 W: J* a, \: I9 H: k2 j2 T2 g

Our study thus supports the hypothesis that VSELs constitute a “mobile” pool of primitive/pluripotent stem cells that could be released from the BM into the PB under the influence of various physiological or pathological stimuli.
我们的研究支持了假设，即存在VSELs 构成了一个可移动的 primitive/pluripotent stem cells 来源，它们在多种因素（生理或病理的刺激）的作用下从骨髓中被释放进入外周血液。
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本研究结果支持了研究假设，即VSELs是一个“可迁移的”primitive/pluripotent stem cells群落，各种生理或病理刺激可以动员这些细胞从骨髓释放入外周血液。
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仅供参考。