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本帖最后由 hyde 于 2011-1-21 21:53 编辑 # {4 S+ v" ~3 v$ |, q9 M4 ~3 t
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Pig Epiblast Stem Cells Depend on Activin/Nodal Signaling for Pluripotency and Self-Renewal8 L/ A; `: B5 b. Q! |$ c+ s
pEpiSC通过Activin/Nodal Signaling信号途径来维持多能性和自我更新能力4 ^! G6 ?$ ~' n/ w% k) p. C* t
* \, o3 d' w S0 }# ], ^Ramiro Alberio , Nicola Croxall , and Cinzia Allegrucci
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1.说明：原文来源自http://www.liebertonline.com/doi/abs/10.1089/scd.2010.0012* t. [" j: y6 g* ~* R& R* l. G
由干细胞之家新闻小组成员Hyde翻译（转帖请注明）
) H# {+ P% a0 `7 D7 z, G4 ?4 l2.翻译内容
! K% A/ X' O5 VmEpiSC和hESC通过Activin/Nodal signaling信号途径来维持细胞的多能性和自我更新能力。我们想要通过研究获知该信号系统在pEpiSC中的作用。我们取10.5-12天的猪胚来建立pEpiSC细胞系。从获得的胚胎中分离出epiblast。其中使用小鼠灭火成纤维细胞（MF）做为饲养细胞，并在培养基中补充bFGF。获得的pEpiSC表达多能性核心标准因子，如 OCT4 (或POU5F1 )、NANOG 、SOX2 和 NODAL。但是监测不到 REX1 和AP（碱性磷酸酶）活性。为了研究 leukemia inhibitory factor (LIF)/JAK/STAT3 pathway信号途径对pEpiSC多能性和自我更新能力的影响，我们在培养基中分别加入特异性的抑制剂JAK I inhibitor 420099和LIF的抗体。pEpiSC的多能性在多次传代培养（>3）后都未出现明显的变化。相反，在培养基中加入针对 Activin/Nodal pathway的抑制剂Alk-5 inhibitor B431542，pEpiSC出现向神经细胞分化的现象。 pEpiSC 是具有多能性的，它们可以自发分化为内胚层、外胚层、中胚层组织。在诱导条件下如BMP-4，还可以分化为滋养层细胞和生殖系细胞前体。我们的研究发现猪的 epiblasts 能够表达多能性的核心标准因子，同时 pEpiSC 的自我更新能力与Activin/Nodal signaling信号途径相关。这项研究也进一步证明了通过Activin/Nodal signal信号途径来保持多能性在不同的哺乳生物之间具有一定的保守性。8 a8 y. \! r) |9 b h
3.原文
% I* m. s- \. y8 [Activin/Nodal signaling is required for maintaining pluripotency and self-renewal of mouse epiblast stem cells7 D4 H) J4 V# R; m
and human embryonic stem cells (hESC). In this study, we investigated whether this signaling mechanism is
7 L: m. }# y- E: P* n) O" Calso operative in cultured epiblasts derived from Days 10.5–12 pig embryos. Pig epiblast stem cell lines (pEpiSC)
$ V- E7 [& O7 v: J1 kwere established on mouse feeder layers and medium supplemented with basic fibroblast growth factor (bFGF).
, V b6 G1 t4 C: p: W: w: @, X WpEpiSC express the core pluripotency factors OCT4 (or POU5F1 ), NANOG , SOX2 , and NODAL , but they do not: o i T5 V& o* ?6 S* P9 h
express REX1 or alkaline phosphatase activity. Blocking leukemia inhibitory factor (LIF)/JAK/STAT3 pathway
8 o! X! {' d8 A- m1 T9 y! z% nby adding the specific JAK I inhibitor 420099 and an anti-LIF antibody over 3 passages did not affect pluripotency
& P3 L5 O" I6 L9 H' W3 zof pEpiSC. In contrast, cells grown with the Alk-5 inhibitor SB431542, which blocks Activin/Nodal
9 w3 w1 ?2 O- A t7 z$ w" W. ~6 ^pathway, differentiated readily toward the neural lineage. pEpiSC are pluripotent, as established by their differentiation0 z. z; w( u3 H5 s- e6 T i
potential to ectoderm, mesoderm, and endoderm. These cells can be induced to differentiate toward3 z6 t2 o$ m9 @# @0 I
trophectoderm and to germ cell precursors in response to bone morphogenetic protein 4 (BMP-4). In conclusion,
& F" `& z* g9 e- dour study demonstrates that pig epiblasts express the core pluripotency genes and that the capacity for maintaining! w1 |0 E% i. Y$ ^! U
self-renewal in pEpiSC depends on Activin/Nodal signaling. This study provides further evidence that
1 E9 J0 \0 K4 B4 Y4 kmaintenance of pluripotency via Activin/Nodal signal is conserved in mammals.. Q& f. r9 H: g7 p
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发表于 2011-1-21 21:15
发表于 2011-1-21 21:34
