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Donor-matched comparison of dental pulp stem cells and bone marrow-deriver mesenchymal stem cells in a rat model, N Z2 S4 }. H y
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摘要： `7 v( H2 j5 h# J6 G% T
关于DPSC和BMMSC间有过很多的对比研究。但是，这些研究的结果存在很多的争议，而引发这些争议的，有可能是因为供体来源的多样性。, V7 s9 J" }, S2 F# w' R
在这个试验中，我们使用相配的供体去研究两者之间的生物学特性。所有试验中使用的细胞都是从同一个SD大鼠中获得。: N+ B1 f& r, a" W1 ]
我们实验结果显示两者具有相似的形态学特征和流式细胞术特点，都能在体外形成克隆，都可以分化为成骨细胞、成软骨细胞和成脂肪细胞。然而，定量分析发现，DPSC具有更快的细胞倍增时间和更高比例的祖细胞（通过细胞克隆形成实验）。再者，体外成骨诱导培养液培养3周后，DPSC有更高的ALP活性。/ |& j) ^* D% z5 ^1 Z) ~& V
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Abstract/ K4 Q$ [% H) i7 h. L
Dental pulp stem cells (DPSCs) have drawn much interest for the regeneration of mineralized
6 ~3 V7 i& F5 \7 P9 A+ gtissues, and several studies have compared DPSCs to bone marrow-derived mesenchymal stem cells (BMMSCs). However, conflicting results, possibly due to donor-associated variability, have been published and the regenerative potential of DPSCs is currently unclear. In the present study we have sought to address this problem using a donor-matched experimental design to robustly compare the biological properties of DPSCs and BMMSCs. All experiments were performed using cells isolated from a single adult Sprague–Dawley rat. Our results show that DPSCs and BMMSCs had similar morphologies and flow cytometry profiles, were capable of forming colonies in vitro and were capable of osteogenic, chondrogenic and adipogenic differentiation. However, quantitative comparisons revealed that DPSCs had a faster population doubling time and a higher percentage of stem/progenitor cells in the population, as determined by clonogenic assays. Furthermore, while both cell populations formed mineral in vitro, DPSCs had significantly higher alkaline phosphatase activity than BMMSCs after 3 weeks in osteogenic medium. These data show several key differences between DPSCs and BMMSCs and support the possibility of using DPSCs for mineralized tissue regeneration. Copyright 2009 John Wiley & Sons, Ltd. |
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发表于 2011-2-12 20:01
发表于 2011-2-15 23:49
