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The odontogenic potential of STRO-1 sorted rat dental pulp stem cells in vitro3 m4 T# o! b M/ c# P2 i0 g) W
摘要：/ _- t; \; z8 w9 Y
牙髓细胞中包含了多种不同的细胞，这可以解释为什么很多体内外实验会有不同的结果。+ c9 S9 W& D5 F2 y' L0 q- {
在这个研究当中，我们利用STRO-1对大鼠的牙髓细胞进行流式细胞分选术FACS。然后对分选出来的细胞进行成牙能力的检测。STRO-1+、 STRO-1-及没有分选的三种细胞在成牙诱导液中培养（含有地塞米松和β-甘油磷酸酯），用扫描电镜观察，检测增值率、ALP活性和钙结节。( d5 w1 S( y5 o, S2 k7 F! Q: z
结果显示，STRO-1+细胞可以分化为成牙本质细胞，未分选的细胞呈现相同的结果。而STRO-1-的细胞则呈成纤维细胞样。SEM及RT-PCR都能证实上述结果。
. l% Z' }. v; Q, P总的来说，利用STRO-1能从大鼠中分选出更纯的能利用的细胞，可以分化为成牙本质细胞，STRO-1-细胞则不能。但是STRO-1+细胞和未分选细胞之间的实验结果并没有明显的差异。
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Abstract& D$ w: p+ B. s! x
The presence of heterogeneous cell populations in dental pulp may count for the considerable2 q4 `7 y2 C( x# A7 a
variation in the outcome of in vitro and in vivo experiments. Here, we intended to determine! Z, B& d ]; H- f
whether a minor cell sub-population of high proliferation and odontogenic potential existed among a larger compartment of perhaps more committed progenitors. In this study, the STRO-1 antigen, defining a mesenchymal stem cell or progenitor subpopulation, was used for separating rat dental pulp cells with fluorescence-activated cell sorting (FACS). Subsequently, the STRO-1 positive cells were tested for their ability to differentiate towards an odontoblast-like phenotype. Three cell populations (STRO-1 positive, STRO-1 negative, and non-sorted cells) were cultured in odontogenic medium containing dexamethasone and β-glycerophosphate. Cultures were analyzed by light- and scanning electron microscopy (SEM), and assessed for proliferation, ALP activity, and calcium content. Results showed that the STRO-1 positive cell population was able to differentiate into the odontoblast phenotype, similar to the non-sorted population. The negative cells however showed a fibroblast-like phenotype. SEM and real-time PCR confirmed such results. In conclusion, the
L* O8 U2 b5 i# _$ iSTRO-1 selection proved applicable for rat-derived material, to obtain a cell population which is. A: K2 ]$ W0 j2 s
more homogeneous. This positive cell fraction was capable of differentiating into the odontogenic pathway, whereas the negative fraction was not. However, the effect was not always advantageous, when compared to non-sorted cells. Copyright 2007 John Wiley & Sons, Ltd. |
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发表于 2011-2-12 20:04
