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Stem Cells International. ?4 b5 `, r6 E) C r) V
Volume 2011, Article ID 504723, 14 pages2 S5 m7 S4 L9 P4 A* @' x
doi:10.4061/2011/504723- f6 M0 {, G8 i7 X1 n3 B
“Humanized” Stem Cell Culture Techniques:
8 w3 [& V' p& J/ V. u4 F) G0 ^The Animal Serum Controversy
. Y. W7 |2 N, H# K* F4 v0 V, t7 KCellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to6 E ~- B8 K8 a
regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow,
" c4 p c8 f; v( ?$ v; V- Padipose tissue, and so forth, bring forth the need for their in vitro culture/expansion. However, the extensive usage of supplements/ r% L- g& ^% W; r/ h5 \5 b' X" j2 T# O
containing xenogeneic components in the expansion-media might pose a risk to the post-transplantation safety of patients." m2 |% x% I" D$ g8 {2 X6 c
This warrants the necessity to identify and develop chemically defined or “humanized” supplements which would make in vitro
z9 F* G& h9 F9 _cultured/processed cells relatively safer for transplantation in regenerative medicine. In this paper, we outline the various caveats
, c1 x% P& b8 n) f: r7 A$ zassociated with conventionally used supplements of xenogenic origin and also portray the possible alternatives/additives which
- {1 Y* E, c( a, }8 lcould one day herald the dawn of a new era in the translation of in vitro cultured cells to therapeutic interventions. |
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发表于 2011-4-11 16:58
