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本帖最后由 hyde 于 2011-5-3 00:49 编辑
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Risk assessment of porcine reproductive and respiratory syndrome virus (PRRSV) transmission via somatic cell nuclear transfer (SCNT) embryo production using oocytes from commercial abattoirs9 C/ x m3 |' L8 j
使用商业屠宰场获取的卵巢进行猪SCNT传播PRRSV的风险评估" I+ J, d, q# g& G/ i5 `. D0 {
原文来自于http://www.sciencedirect.com/sci ... ce&searchtype=a" a& B" K! Y2 W2 Z7 X
由干细胞之家Hyde翻译，转帖请注明，谢谢……% T6 Z6 ~8 n+ s
摘要：6 f- n. w& h- g8 A( ?1 f
SCNT是生殖生物学的有力工具，它可以保存和传播（繁殖）动物的优良基因【无性繁殖】。使用SCNT获得胚胎首先需要去除卵母细胞的细胞核然后注入成体供体细胞。这个过程在透明带上产生了几个开口，提高了SCNT胚胎病毒感染的几率。SCNT使用的卵母细胞大多数来自于商业的屠宰场，这些屠宰场必然是存在病毒污染的。因此，在大规模地将这一技术实施于畜牧产业之前我们需要建立系统的用于疾病传播风险评估的体系。本研究的目的是通过检查现有的因猪SCNT胚胎传播PRRSV的情况来评估SCNT产生胚胎传播疾病的风险。SCNT产生胚胎的每一个环节都使用RT-PCR进行PRRSV的监控，该RTPCR检查系统敏感性是个5拷贝，具体环节从供体细胞到移植前的SCNT胚胎。7年里我们所用猪皮肤组织建立的114株供体细胞系经检查都是PRRSV阴性。从736个卵巢中获得68份PFF样，其中只有4份（5.9%）是阳性，说明只有少数卵母细胞供者含有病毒分子（卵母细胞供者只含有少量病毒分子）。四次独立实验产生的801个Day7 SCNT 胚胎检查为阴性，67批次获得的超过11571个 washed oocytes （？）检查为阴性。这些卵母细胞来自于多个处理动物的屠宰场，这些屠宰场分布在猪密度比较高的地区，对应的供体数量超过了5828.这些结果说明了屠宰场的卵母细胞是没有被PRRSV感染的，能够被安全地用于体外胚胎的产生。另外，建立完善的SCNT胚胎产生体系即包括供体细胞的测试、卵母细胞的去污染、无病原体胚胎的产生和培养就没有传染PRRSV的风险。
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Somatic cell nuclear transfer (SCNT) technology has become a powerful tool for reproductive biology to preserve and propagate valuable genetics for livestock. Embryo production through SCNT involves enucleation of the oocyte and insertion of a somatic donor cell into the oocyte. These procedures lead to a few small openings on the zona pellucida that may elevate risk of viral infection for the produced SCNT embryos. The oocytes used for SCNT are mainly obtained from abattoirs where viral contamination is almost inevitable. Therefore, a systematic evaluation of risk of disease transmission through SCNT embryo production is necessary prior large scale implementation of this technology in the livestock industry. The objective of the current study was to evaluate the risk of disease transmission via SCNT embryo production and transfer by testing for the presence of porcine reproductive and respiratory syndrome virus (PRRSV) throughout the process of SCNT embryo production. The presence of PRRSV in each step of SCNT embryo production, from donor cells to pre-implantation SCNT embryo culture, was carefully examined using a real-time PCR assay with a sensitivity of five copies per-reaction. All 114 donor cell lines derived from pig skin tissue over a period of 7 years in our facility tested negative for PRRSV. Out of the 68 pooled follicular fluid samples collected from 736 ovaries, only four (5.9%) were positive indicating a small amount of viral molecule present in the oocyte donor population. All 801 Day 7 SCNT embryos produced in four separate trials and over 11,571 washed oocytes obtained in 67 batches over 10 months tested negative. These oocytes were collected from multiple abattoirs processing animals from areas with high density of pig population and correspond to a donor population of over 5828 individuals. These results indicate that the oocytes from abattoirs were free of PRRSV infection and therefore could be safely used for in vitro embryo production. Additionally, the established SCNT embryo production system, including donor cell testing, oocytes decontamination, and pathogen free embryo reconstruction and culturing, bears no risk of PRRSV transmission.7 _/ `- ^8 V: Q3 V7 o* Y/ R% y$ U
虽然国内国外有差别，但最重要的是做好最后那句话……还有就是国家对传染病的态度……口蹄疫是病不是钱袋; ], H+ Q/ M" [5 }& K
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发表于 2011-5-3 00:47
发表于 2011-5-3 07:59
