本帖最后由 细胞海洋 于 2016-1-15 12:28 编辑 4 p e- Q& R! I6 H ! D# C R* p" |- T$ e5 B( O' F- L作者: 蓝枫麦麦 时间: 2016-1-15 12:56
:):)作者: lchanlon 时间: 2016-1-15 22:52
Thanks for your input作者: mecol 时间: 2016-1-16 08:12
thank u作者: zhangweihwz 时间: 2016-1-16 11:48
Thank you for sharing.作者: ppcl2 时间: 2016-1-16 15:10
多能干细胞基因组编辑作者: mrstem 时间: 2016-1-18 10:32
看这题目就值得学习!作者: ROYY 时间: 2016-1-21 22:15
学习下作者: qqganxb 时间: 2016-1-23 00:57
看个帖子真麻烦作者: xiaoheilong 时间: 2016-1-23 16:00
感谢分享!作者: qqganxb 时间: 2016-1-24 00:19
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1月6日,Cell子刊《Cell Stem Cell》在线发表了来自哈佛大学和麻省总医院研究人员的一篇综述文章,题为“Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions”。 . y( ?8 f$ V. D% j- U ( k6 X3 V9 U- g. \% N. b% MGenome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions @7 j( ^! K9 j4 DSummary: Human pluripotent stem cells (hPSCs) with knockout or mutant alleles can be generated using custom-engineered nucleases. Transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 nucleases are the most commonly employed technologies for editing hPSC genomes. In this Protocol Review, we provide a brief overview of custom-engineered nucleases in the context of gene editing in hPSCs with a focus on the application of TALENs and CRISPR/Cas9. We will highlight the advantages and disadvantages of each method and discuss theoretical and technical considerations for experimental design.8 d- g$ y3 g+ Z
2 \7 K g1 m5 J# `# l7 p' h6 _作者: addstemcell 时间: 2016-1-25 11:23