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Hongkui Deng! x7 L0 K# y6 J3 `; W5 X
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Laboratory of Stem Cell and Generative Biology, College of Life Sciences, Peking University, Beijing 100871, China. : ^* C$ z2 a4 ?. |' V. L6 o5 A. j2 i! ]
Human pluripotent stem cells represent a potentially unlimited source of functional pancreatic endocrine lineage cells. We established a new highly efficient approach to induce human embryonic stem （ES） cells and induced pluripotent stem （iPS） cells to differentiate into mature insulin-producing cells in a chemical-defined culture system. The differentiated cells obtained by this approach comprised nearly 25% insulin-positive cells as assayed by flow cytometry analysis, which released insulin/C-peptide in response to glucose stimuli in a manner comparable to that of adult human islets. Most of these insulin-producing cells co-expressed mature beta cell-specific markers such as NKX6-1 and PDX1, indicating a similar gene expression pattern to adult islet beta cells in vivo. This work provides a new model to study the mechanism of human pancreatic specialization and maturation in vitro, and enhances the possibility of utilizing patient-specific iPS cells for the treatment of diabetes 5 M+ {: l. M5 f - c c2 D0 T8 ^5 x回复为ppt $ e) A/ |0 z, [: W[attach]1345[/attach] % s( d; R+ I5 C5 R8 E7 W[attach]1346[/attach]0 Z$ @ ^/ J. ]
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[attach]1344[/attach]作者: bobo 时间: 2009-6-28 08:36
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[attach]1355[/attach] 9 h3 B B3 P* d[attach]1349[/attach]作者: bobo 时间: 2009-6-28 08:39
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[attach]1356[/attach] " r# X% a# x% k$ \[attach]1362[/attach]作者: bobo 时间: 2009-6-28 08:41