' B" m# w. F5 j% B! C c4 P3 z表观遗传学与干细胞——2009年亚洲生物高峰论坛录制(音频mp3) + W3 B* w- D- Z& k7 C& D i" P! b* K8 W0 U% H( ~. L5 I C. E8 R7 w7 Z& o
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回复为ppt : h+ V; Q1 {# t l3 K+ z" k3 g [$ y: ^摘要： . w2 I5 J( M9 [ ! b7 ^! N: {$ ?/ ^& _6 m- XHongkui Deng" F8 ?. J2 X) o5 k4 L) ]
! f* {) B q- Z% rLaboratory of Stem Cell and Generative Biology, College of Life Sciences, Peking University, Beijing 100871, China.9 ~7 r8 V- O$ j; I; b
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Human pluripotent stem cells represent a potentially unlimited source of functional pancreatic endocrine lineage cells. We established a new highly efficient approach to induce human embryonic stem （ES） cells and induced pluripotent stem （iPS） cells to differentiate into mature insulin-producing cells in a chemical-defined culture system. The differentiated cells obtained by this approach comprised nearly 25% insulin-positive cells as assayed by flow cytometry analysis, which released insulin/C-peptide in response to glucose stimuli in a manner comparable to that of adult human islets. Most of these insulin-producing cells co-expressed mature beta cell-specific markers such as NKX6-1 and PDX1, indicating a similar gene expression pattern to adult islet beta cells in vivo. This work provides a new model to study the mechanism of human pancreatic specialization and maturation in vitro, and enhances the possibility of utilizing patient-specific iPS cells for the treatment of diabetes' v6 U# o/ ?# M9 Q; v+ }
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[attach]1348[/attach] $ H2 r( {4 U8 k ^; }7 [( j[attach]1344[/attach]作者: bobo 时间: 2009-6-28 08:36