, M' q0 b+ I, gInduced pluripotent stem cells (iPSCs) outwardly appear to be indistinguishable from embryonic stem cells (ESCs). A study of gene expression profiles of mouse and human ESCs and iPSCs suggests that, while iPSCs are quite similar to their embryonic counterparts, a recurrent gene expression signature appears in iPSCs regardless of their origin or the method by which they were generated. Upon extended culture, hiPSCs adopt a gene expression profile more similar to hESCs; however, they still retain a gene expression signature unique from hESCs that extends to miRNA expression. Genome-wide data suggested that the iPSC signature gene expression differences are due to differential promoter binding by the reprogramming factors. High-resolution array profiling demonstrated that there is no common specific subkaryotypic alteration that is required for reprogramming and that reprogramming does not lead to genomic instability. Together, these data suggest that iPSCs should be considered a unique subtype of pluripotent cell.' W7 f' {* f$ K% q5 W+ i, f
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3楼原文 感谢zpzp0312提供作者: hualin840518 时间: 2009-7-9 16:24
Logical Biology just published a paper entitled "Published Insights for Distinctions between iPSCs and ESCs and Why and How". http://bit.ly/2CEnjh作者: have-a-rest 时间: 2009-7-23 09:49
本帖最后由 have-a-rest 于 2009-7-23 09:52 编辑 1 d9 S O" C b9 d" o/ c( ?5 E# G: `. M+ R; H. X$ e
据Shinya Yamanaka分析，不同来源的ips在某些功能、表观状态等方面是不同的。与ES比较，他认为：ES=MEF-ips>=STM-ips>HEP-ips>>TTF-ips。 ' c: l" r6 y: i% P具体见他的文章。Variation in the safety of induced pluripotent stem cell lines作者: luohucy 时间: 2009-8-14 10:38