标题: 求助current protocols in stem cell biology [打印本页] 作者: 大漠 时间: 2009-7-28 16:37 标题: 求助current protocols in stem cell biology
Current Protocols in Stem Cell Biology( ]+ m7 h! l# [1 q! j1 o: d
Last Update: July 07, 2009 ! A) ] K6 Q; B3 ZPrint ISSN: 1941-7322 7 e- V K9 b! w" [. q5 wOnline ISSN: 1938-8969 4 q$ s6 K. F2 I( ]9 iTable of Contents+ i! ~, f6 t2 f$ F( Z$ }3 G! h
Foreword : \) e1 s! a+ p/ P! bPreface / b' ^* E+ ]8 E5 g& y Chapter 1 Embryonic and Extraembryonic Stem Cells / f$ |1 o) ~. B7 D8 Q, X+ T: v
Section A Isolation of Embryonic Stem Cells 6 h% t6 O# s# _/ aIsolation of Embryonic Stem Cells 5 B! n8 s- S' u6 J( @ e
UNIT 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells # d5 ~+ I e8 i3 `UNIT 1A.2 Derivation of hESC from Intact Blastocysts $ Z7 S3 U- p+ J8 c. x0 G
UNIT 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells I: v- i0 X& t( b
UNIT 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment 5 h7 [3 j8 ]+ S5 u# ~
Section B Characterization of Embryonic Stem Cells 7 C6 ` Y l7 i$ g& P Y/ y; h5 Z" @UNIT 1B.1 Proteomic Analysis of Pluripotent Stem Cells 4 `7 l5 {) T7 A, W( i. x0 ]
UNIT 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform . \5 U) \" \5 p2 VUNIT 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells 7 m2 R! P, M. U8 QUNIT 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency - k0 r, L1 ^5 |UNIT 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation 0 K2 c+ D" |& F1 a. ?) z3 T Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells - I* u9 N5 j' {5 V' N
Culture and Maintenance of Undifferentiated Embryonic Stem Cells 3 r4 j3 t% Y; W8 f* wUNIT 1C.1 Expansion of Human Embryonic Stem Cells In Vitro % j# U" @3 ^) S6 nUNIT 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture * p1 [5 D+ m& ^, h1 Y. t/ yUNIT 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells & ^ p$ T4 O2 e, w
UNIT 1C.4 Culture of Mouse Embryonic Stem Cells 4 r9 O3 z$ H- @ m6 P8 @UNIT 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells 4 B. R5 W- d$ C/ h" f) JUNIT 1C.6 Isolation of Human Placental Fibroblasts 3 r9 K& K* o/ I% L/ R% J/ j, x+ ]
UNIT 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells ( ^' I# _9 ?, C3 |$ ]
UNIT 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor 6 N* U$ n; Z; f; v Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells 3 C4 O/ E' p6 a: i; P: T6 ^! DUNIT 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap ' h6 f! [ F3 ~8 A( E' \UNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures , Y* M N( Q# f5 M& H0 J; @: kUNIT 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay 8 V( c0 F0 q7 ~1 j9 w* i3 @& K4 L P4 uUNIT 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions ; v$ H; o) q ^* m, D4 L6 T
UNIT 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells ) v% ^ h9 L8 |# P5 E0 F
Section E Extraembryonic Lineages ) I/ u+ ?6 t' u' b% m3 \6 \
Isolation of Stem Cells from Extraembryonic Tissues * a9 Z @5 }8 I- n& i. i3 o
UNIT 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells ! _" p+ H0 v, @
UNIT 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta " q0 u+ q+ D1 Q
UNIT 1E.3 Isolation of Amniotic Epithelial Stem Cells % `8 p2 L% v9 M6 F, ?
UNIT 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells ' g/ x. h: e4 S, s7 }+ P
Section F Mesodermal Lineages $ o/ o8 |5 `' y9 A z" M) T2 `
UNIT 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells # n2 L0 Z: G) oUNIT 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium " P2 r2 w, V/ m9 j. F0 }% Z. b' k
UNIT 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells : j0 ^! k, r: }UNIT 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood * v% n4 Z$ V% LUNIT 1F.5 Endothelial Differentiation of Embryonic Stem Cells 8 w% r, w% V# I. e: z8 n9 b
UNIT 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers i4 o3 _1 |! k7 VUNIT 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors ) `+ L U0 t3 S7 c3 T
UNIT 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells 5 d3 R6 L0 \9 X+ ] Section G Endodermal Lineages # {- h3 x* e. j' \! G; M, c1 e
UNIT 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells . V" J; Z( o+ x1 Y- A1 u
UNIT 1G.2 Pancreas Differentiation of Mouse ES Cells 4 ~& T3 I1 }3 j( ^- OUNIT 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm + {! a4 h1 d+ X2 r3 a, o
Section H Ectodermal Lineages " S. o' h" W( Z7 x" OUNIT 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons ) d8 Q3 u8 b$ g, Y: |2 ?2 b
Chapter 2 Somatic Stem Cells 6 t+ m, q8 i/ d% c* P( C
Section A Hematopoietic Stem Cells 8 V/ {# v) ?4 s/ hHematopoietic Stem Cells 6 f8 i: G% M" Z. L
UNIT 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient 3 f8 ?! h$ \- Y V5 PUNIT 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood * r' P" |' P- g2 C
UNIT 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood 6 P e2 F. e; ^' \& @+ F; W$ `" YUNIT 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow . S6 x2 M3 |! m4 h+ ^8 H% j
UNIT 2A.5 Analysis of the Hematopoietic Stem Cell Niche I1 ?- i4 T0 x# Z( {
UNIT 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues + m! `4 f% K4 g3 s1 ~( ~/ ~9 @! T
UNIT 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells : x& n5 D: I0 P2 J3 }' T: aUNIT 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells , S/ g4 j& n$ u$ d9 P1 @/ f% G
Section B Non‐Hematopoietic Bone Marrow‐Derived Stem Cells 0 F5 I( W. p6 `0 Z+ u; M' |
UNIT 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues ' N' |9 j. y* Z" q
UNIT 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells 8 @. S. T( V+ B; qUNIT 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells 0 E& G8 ~# D( |: W j: k
Section C Cardiovascular Stem Cells % |" {: {2 g- d+ Q; lUNIT 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood ( @3 k" x6 j1 r6 S) y0 gUNIT 2C.2 Derivation of Epicardium‐Derived Progenitor Cells (EPDCs) from Adult Epicardium ' S2 E2 O4 u7 {" A) N3 w0 J Section D Neural Stem Cells 1 z4 S! e* F' U& V1 M4 T7 LUNIT 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains 0 Z2 T; L; t$ }; ?
UNIT 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells 4 v: B% V5 D: |# A. O8 C' b6 HUNIT 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells + d& Q/ c% h: h. a4 v0 @9 |6 E
Chapter 3 Cancer Stem Cells ( L& K& w' M) o# J7 F( m I1 uUNIT 3.1 Colon Cancer Stem Cells 2 D' h: e2 N$ q# X$ w1 a5 `3 T$ p! f
UNIT 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model : Q/ C" `6 ^2 r U+ X Chapter 4 Manipulation of Potency 3 _; V) v7 ?4 b# t- p$ d$ g8 ?" A
Section A iPS Cells 6 h# r" U; i% g. t4 G. J! X
UNIT 4A.1 Human iPS Cell Derivation/Reprogramming ) t! A% m [# p. u2 _) ~
UNIT 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells 9 ^" o z) ?' I! v
Section B Nuclear Transfer 9 r! U% ^+ q2 p% \% E: g" ^UNIT 4B.1 Heterokaryon‐Based Reprogramming for Pluripotency 4 l& E c2 a) q9 _* _) R6 ?
Chapter 5 Genetic Manipulation of Stem Cells % @/ X/ G4 g+ { Section A Lineage Tracers in Stem Cells . A8 r w# n% K9 w, A- `8 Q5 M& Y" v
UNIT 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma 2 z4 m2 z' }! A2 N7 z' k+ u, E$ dUNIT 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing ; X g/ }! f* I7 X) c
Appendix 1 Useful Information 6 }( G% g- j5 sAPPENDIX 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research ( ^, a" b; v( ^6 B: u8 U( }
APPENDIX 1B ISSCR Guidelines for the Clinical Translation of Stem Cells 5 @. v% B0 a1 \
Appendix 2 Laboratory Equipment 5 }' X! x$ E, g4 R8 o4 OAPPENDIX 2 Standard Laboratory Equipment " F$ |: m7 G5 A7 A Appendix Suppliers作者: jidatui 时间: 2009-8-29 23:03
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我有UNIT 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
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