6 F5 e' m$ C) X0 ^! a5 W8 rOct4是核抗原，所以triton是必须的，SSEA3/4是膜表面抗原，可加可不加，你为了方便可以都加，把我文章里面的方法发上来给你参考吧。/ r$ }" C* ?+ Y$ h e J
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Immunofluorescence Analysis) g; M# `& P$ i
Cells were fixed with 4% paraformaldehyde for 10 min., incubated in PBS containing 5% rabbit serum and 0.4% Triton X-100 for blocking and permeablization, respectively. PBS containing 0.5% Tween 20 (PBS-T) was used to dilute antibodies and wash the cells in the following procedures. The cells were incubated with antibodies against OCT4, SSEA-4，TRA-1-60 and TRA-1-81 at 37 °C for 1 h, followed by washing with PBS-T for three times. Afterwards, the cells were incubated with fluorochrome-conjugated, corresponding secondary antibodies at 37 °C for 45 min. and washed with PBS-T for three times. Finally, the cells were examined under fluorescence microscope to capture both phase and fluorescent images.作者: xyzengh 时间: 2009-9-8 22:38
本帖最后由 xyzengh 于 2009-9-8 22:44 编辑 * C: a {3 R% {+ Z4 {! u' W
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再附上几张图让你有个感性认识吧。作者: maxiaorongqp 时间: 2009-9-9 00:37