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研究人员表示，该项技术的难点在于如何将多种生物化学成分与可被细胞吸收的紧凑纳米粒子相结合，并稳定地存在，直到其按照需要进行释放。激光控制释放是一个方便而强大的工具，允许对特定细胞释放出精确剂量的药物。近红外光的生物友好型组织渗透法对于将这种能力扩展到较大的生物系统来说是十分理想的。该技术亦可扩展到针对不同的生物目标释放不同的药物分子。 ' }, f6 B$ t" n" |. ` 1 l2 H1 Y! c: F p5 J原始出处：9 |5 S7 i7 T9 z; W8 _4 K
7 ^, e5 S. w6 V. @6 F+ p: D# tACS Nano, 2009, 3 (7), pp 2007–2015 DOI: 10.1021/nn900469q , e% v& }7 s" c }* N; }5 ^# x$ @3 ]$ _ x4 \4 ?
Laser-Activated Gene Silencing via Gold Nanoshell-siRNA Conjugates! A! C1 g2 |# A/ Z+ k# H& g
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Gary B. Braun?, Alessia Pallaoro?, Guohui Wu?, Dimitris Missirlis?, Joseph A. Zasadzinski?, Matthew Tirrell? and Norbert O. Reich?* ' `$ e; C9 C) z) \9 [/ O . F8 x2 \! j8 x% u8 G Department of Chemistry and Biochemistry$ j: M6 ~5 l2 |; ~/ i$ B( G4 Y
Department of Chemical Engineering and Materials Research Laboratory, University of California, Santa Barbara, California 93106! Q# Y0 \- s8 A4 u: x
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The temporal and spatial control over the delivery of materials such as siRNA into cells remains a significant technical challenge. We demonstrate the pulsed near-infrared (NIR) laser-dependent release of siRNA from coated 40 nm gold nanoshells. Tat-lipid coating mediates the cellular uptake of the nanomaterial at picomolar concentration, while spatiotemporal silencing of a reporter gene (green fluorescence protein) was studied using photomasking. The NIR laser-induced release of siRNA from the nanoshells is found to be power- and time-dependent, through surface-linker bond cleavage, while the escape of the siRNA from endosomes occurs above a critical pulse energy attributed to local heating and cavitation. NIR laser-controlled drug release from functional nanomaterials should facilitate more sophisticated developmental biology and therapeutic studies.作者: llingzu 时间: 2010-2-3 22:16