Nat Methods. 2009 Oct 18.【Epub ahead of print】 * E! F9 k1 K2 M0 C6 S7 d ?8 l- V
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A chemical platform for improved induction of human iPSCs. ' O v( Y. o3 Y" y8 n4 G ^3 P 2 L/ N% l) a" t1 FLin T, Ambasudhan R, Yuan X, Li W, Hilcove S, Abujarour R, Lin X, Hahm HS, Hao E, Hayek A, Ding S.+ F6 b' F0 {' f$ T3 O
Department of Chemistry, The Scripps Research Institute, La Jolla, California, USA. 6 \4 r4 K0 ~ p5 L5 t: j* ^9 b! i! x+ i. o
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells. ) B0 z, U5 y2 P/ Y& b6 f3 u- W9 S3 R0 c7 M3 _+ g
PMID: 1983816& D, I! T2 N% Q" V
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[attach]2699[/attach]作者: jianlindunev 时间: 2009-10-22 15:52