We performed three additional experiments using CD271& m) p7 f4 L1 W% H! d
for enrichment of MSC. Upon isolation of MNC by Ficoll7 z, m' z% h. }
density fractionation, half of the sample was processed1 v( R$ o' y4 i( ]$ C% K& l+ Z
further using the CD271 MicroBead Kit. About 5% of the cells were in the positive fraction and about 50% of these / Z [+ V* h$ D5 C+ Lwere CD271, as determined by flow cytometry. CFU-F 7 O1 T6 S1 @- [7 O7 s9 z) owere exclusively found in the CD271 fraction, as5 _# I4 I9 Y6 H9 D; ^" _
described elsewhere [22]. The number of CFU-F per " B# W8 Q7 W d" q; Scell was 210 times higher in CD271 cells in comparison0 R5 w* ?# M5 M" g( j& D. I5 I( g
with MNC. Hence MSC were enriched in the CD271, ?0 W0 x( S& o8 l; Y. P0 _ j' c* G
fraction, although the yield of CFU-F per milliliter BM # ^4 ?4 `2 m+ B7 t0 ^1 @aspirate or with regard to the number of MNC was greatly {" k' y2 [2 z% b; U* d( w
reduced.作者: oldmac7 时间: 2010-3-23 14:19