5 r: E- E0 d' Q' a) U* UFOREWORD 4 W8 T5 i: W1 m. wStem cell biology is emerging as a field in biology with tremendous therapeutic potential.( u1 I( {7 y g7 V$ F1 J0 n1 s4 g( b9 X
Making this potential a reality requires an international effort. The recognition+ p0 m3 ]# W/ G7 V( a
that such a promising yet multifaceted discipline needs fostering led to the establishment& [8 o0 G- o1 O7 o
of the International Society for Stem Cell Research (ISSCR). Central to the efforts of the * j* N6 z9 J QISSCR is the development of tools to ensure the success of stem cell researchers. What % A0 P' J- K9 P2 m& ^better way to do this than to collaborate with Current Protocols to develop this valuable # C0 {1 Q$ E' a6 \compendium of protocols in stem cell biology? h+ b$ A6 j$ XStem cell researchers have developed a number of breakthrough techniques, from the 6 @8 S3 q& L" _7 B% H) m+ bderivation and manipulation of pluripotent embryonic stem cells to purification and 9 W) O$ A8 s( y5 G# s& F4 s+ Ztransplantation of tissue-restricted stem cells from adult organs. A number of laboratories * c& B, W5 x) f$ _( F+ n% V. bhave become leaders in the field as a result of developing such techniques. The more) I4 c- D9 Z: {9 F: s
efficient scientists are at implementing new and powerful methodologies in their own ! ~0 l) T2 M5 m& Qlaboratories, the faster stem cell biology will advance our understanding of normal 9 N9 o! `6 L* U8 o8 h- e7 ~# ^development and lead to the development of therapies. Thus, the availability of quality% V; t9 y5 O& H1 T& _8 l
protocols will have amajor impact on the success of the entire field. Current Protocols has * o! c8 U. D: `7 v- O5 Flong been the premier volume for proven in-depth protocols regarding many aspects of ' A) ^( L' E) l9 }0 Abiology, and this volume on stem cell biology will prove a valuable addition to researchers . U3 t7 B; g6 ]& x# n; Jworldwide. - f4 J6 z: X' K% nExperiments in stem cell biology must be interpreted with great caution as well as0 Y8 g, R6 v7 Y! P& h/ | w" _2 o W
openness to alternative explanations. For example, the recently discovered phenomenon + P; a# j1 L" F8 dof cell fusion in vivo or the existence of tissue-restricted blood stem cells in peripheral ' \. V4 j, p$ z0 i' otissues were initially misinterpreted as evidence for stem cell trans-differentiation.) T+ k: D( j( J/ e
It is very important that this compendium of protocols highlight potential pitfalls as well2 }+ ]5 ?& d0 c/ K; r/ @- `& W
as maintain the opportunity for clarification and correction when the need arises. The# H. O1 {$ W" w7 s" Q2 j
fact that these protocols will be provided online will help ensure that researchers always 8 C1 X$ h) g/ M) N( E8 Dhave the latest, most up-to-date protocols available to them.0 Y" J+ X$ E" i7 L5 v/ ]
The stem cell field is burgeoning, and, as I have seen within the ISSCR, there is a genuine2 _$ R& v4 O& b8 V% b* W! Y1 a) D6 j" v
push to share information and interact so that the field can move forward quickly. There. s& l; r4 P0 S% X, c( [! U
is a drive to develop not only excellent basic research skills but to bring the findings* }* y, T' E# S! M$ n5 a
to clinical use so that patients can benefit. As a Hematology Attending Physician at# \# a4 Y% ~6 \) \
Children’s HospitalBoston, I treat children who have pediatric blood diseases or leukemia # |5 _2 S/ R0 o( l7 Qand am drawn by the need to translate our research findings into therapies to help treat a3 p0 o1 M7 T1 n3 h
number of diseases. The promise is great, but we need to deliver, and I believe Current: s; H; \9 R4 A3 C0 }3 `: g2 B8 `
Protocols in Stem Cell Biology will help tremendously.& K3 U; L% p% r$ Q) t4 U! K& d3 M
Leonard I. Zon i2 y1 J+ Z" f' q4 |/ n+ K0 Q& y+ uCurrent Protocols in Stem Cell Biology- _: F {: m1 X5 z3 Y
Published online June 2007 in Wiley Interscience (www.interscience.wiley.com). 2 v9 G. G! n# n" v9 w! qDOI: 10.1002/9780470151808.scfores18 F9 p$ e+ \8 r& a6 [
Copyright C 2007 John * D. G8 I: f! W/ F3 z5 C, Q; j! g0 a/ e" j
附件为pdf全文，感谢原创提供者 ，大家回复之后就可以下载了0 Z! i, c& e0 M# l 作者: xiaodongqin 时间: 2009-3-16 08:51
太好了作者: Jonathan 时间: 2009-3-19 17:38
。。。。。。。。。。。。。。作者: zpzp0312 时间: 2009-3-20 15:01
谢谢楼主！作者: zisu_yu 时间: 2009-3-23 10:29
好东东哦。谢谢啦哈作者: 王乾 时间: 2009-3-23 20:57
感谢你的大力支持！作者: horst 时间: 2009-3-24 08:03
真是好资料，很有帮助，谢谢了作者: dragon513 时间: 2009-4-9 11:48
谢谢分享！作者: hualin840518 时间: 2009-6-10 13:16
作者: bobo10679888 时间: 2009-7-2 22:23
作者: 大漠 时间: 2009-7-28 09:23
太需要啦，谢谢！作者: andey 时间: 2009-7-29 12:03
支持 谢谢分享作者: cdcliwei 时间: 2009-7-29 20:38
1#guokeyi/ }3 ]3 E$ @! ]+ i
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I am waitiing!作者: janechen 时间: 2009-7-30 10:01
谢谢分享！