本帖最后由 细胞海洋 于 2012-10-30 08:51 编辑 D! }( q1 [% Z( _! J
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黄艳欣, 杜雅菊, 李宝杰 $ ?3 Q/ {# m% I/ I+ _9 b5 ?黄艳欣, 杜雅菊, 李宝杰, 哈尔滨医科大学附属第二医院消化内科 黑 # _! r1 l* o- p" n2 e( E# @龙江省哈尔滨市 1500865 q. e6 v; j# Y2 @5 d: l
黄艳欣, 女, 1977-11-14 生, 黑龙江省哈尔滨人, 汉族. 2001 年哈尔滨医科* W3 |2 q* b0 m- L2 [
大学硕士生.& b' i% {9 ^! v8 Z) _8 Q
项目负责人: 杜雅菊, 150086, 黑龙江省哈尔滨市, 哈尔滨医科大学附属第! | V& s5 Z/ p
二医院消化内科.9 ~3 G$ Y a2 ?. q
电话: 0451-866051431 D5 T% _* d( }3 ~
收稿日期: 2003-12-10 接受日期: 2004-02-01( }7 G: p+ u, U' M1 A8 D7 \
Effect on function of rat hepatocytes 7 W2 X0 w: ~+ @. \2 C' C) Bcultured with bone marrow cells $ M* H: y' V: s, S+ pYan-Xin Huang, Ya-Ju Du, Bao-Jie Li ( y6 O: U1 F1 v1 O) mYan-Xin Huang, Ya-Ju Du, Bao-Jie Li, Department of Gastroenterology, 4 j$ X: a& [- y# OThe Second Hospital of Harbin Medical University, Harbin 150086, , A* ^! e" N6 D# aHeilongjiang Province, China/ V" ^# n: w/ G. T/ R6 A
Correspondence to: Dr. Ya-Ju Du, Department of Gastroenterology, 0 e' [5 [( T H2 W7 C. a$ SThe Second Hospital of Harbin Medical University, Harbin 150086,( c8 U9 I* Y0 Z t
Heilongjiang Province, China ; \5 `. [7 S' W1 e6 j, @" kReceived: 2003-12-10 Accepted: 2004-02-01 , _) i2 j, A; q" P- lAbstract , F5 q; ]% u( n2 Y1 W; u NAIM: To study of the effect on the function of rat hepatocytes % {# {( N- Z# U5 D9 H8 ucultured with bone marrow cells.0 b" m1 K+ c' ?
METHODS: Rat hepatocytes were isolated by the modified# U( C1 L) E. _$ w7 H$ ]( D
two-step method described by Seglen. The primary cultured- } @' w( D: X
hepatocytes and bone marrow cells were served as cocultured: l5 \- i& X9 f4 z* L
group, and single cultured hepatocytes as control group. The) F! W C0 h8 O
yield and viability were assessed by trypan blue exclusion. / y7 M x# C! w9 O$ ^$ Q. wThe morphologic changes of cultured hepatocytes were( C' B' I1 E' S, M
observed. The concentrations of albumin and urea in the : O7 i; L) v% N/ [" q" Asupernatant in different cultural period were examined.- |) w- w. u7 Q" L |8 B6 h) L
RESULTS: The albumin synthesis (13.75>2.179, P <0.05)& G3 r0 G% y- y. L: ~+ G
and urea level (7.27>2.179, P <0.05) had fluctuating3 n4 i* W' T/ J- h% }0 A* V
changes in one week, and cocultured group had higher ' V3 i. p* {, Dalbumin synthesis and urea level.' w( F+ Z; H9 f; T- V1 J
CONCLUSION: Cocultured hepatocytes with bone marrow 6 I) p$ R c0 x: y! Jcells can improve the function of hepatocytes. - x/ u( F7 y6 f+ WHuang YX, Du YJ, Li BJ. Effect on function of rat hepatocytes cultured with 6 k2 j& M/ Y$ U% K/ H6 wbone marrow cells. Shijie Huaren Xiaohua Zazhi 2004;12(5):1129-1131 i0 G' D: j7 H) H9 C) `& s5 |摘要6 d% ^8 u& ?- [9 C7 u
目的: 利用原代培养的大鼠肝细胞与骨髓间质干细胞共同培0 K( I% B- m& X/ I2 p; w. }4 m
养研究骨髓间质干细胞对肝细胞功能的影响以便更好! }! m8 q5 X0 [8 }% D! j
用于肝细胞移植及生物人工肝. 0 t' P* G9 H8 }) Z方法: 采用低浓度胶原酶原位循环灌流法分离大鼠肝细胞4 o7 T/ @$ ]9 f% \( u- E
获得有活性的肝细胞进行原代培养. 台盼蓝排斥法计算细胞 ! e3 b$ o8 L, r5 }0 I* G产量和细胞活性; 光镜下动态观察细胞形态学改变并收集不同时期培养上清检测其细胞分泌等功能. 比较肝细胞单/ Y6 ?) M$ v4 O, e$ T+ y
纯培养及其与骨髓间质干细胞共同培养时细胞的功能. 8 u2 O( V# ~/ _! f' F; F" D! f$ V- H结果: 培养7 d时两组均仍然维持白蛋白分泌及尿素合成功4 m: C0 Q$ `% ^" v
能; 共同培养组与单纯肝细胞培养组在白蛋白分泌 (13.75>% S4 z8 V) \/ x+ s/ K* W4 ~
2.179, P <0.05) 及尿素合成功能 (7.27>2.179, P <0.05) 存在8 S: f( b' |; @/ c
显著性差异共同培养组明显高于单纯培养组. 7 A- F' x1 d* j' i: Z结论: 肝细胞与骨髓间质干细胞共同培养可以使肝细胞2 I: A/ Y, A9 S
维持特异性功能提高肝细胞活性.0 ?5 d- Q7 _* j& z& c3 n( \