本帖最后由 细胞海洋 于 2010-5-16 17:10 编辑 ' y8 l- d* F, } n! l! Z# n
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侯玲玲1 , 郑 敏1 , 王冬梅1 , 袁红丰1 , 李海民1 , 陈 琳1 , 白慈贤1 , 张 涌2 , c4 b7 y4 @- x% [ L; l% o裴雪涛1 , 3& a/ c8 N2 @' Z% A6 p ~7 t
1 军事医学科学院输血研究所、军事医学科学院干细胞中心, 北京100850 ; 2西北农林科技大学胚胎工程实验室, A# \, A2 q& n# Q8 U% F: V陕西省杨凌712100 + y+ u/ \5 B/ J4 Y( M3 T1 s( H摘 要: 骨髓间充质干细胞(mesenchymal stem cells , MSCs) 是目前备受关注的一类具有多向分化潜能的组织干细胞,$ t) _+ _; q5 ^
体外可以分化为骨、软骨、脂肪等多种细胞。因此, MSCs 是细胞治疗和基因治疗的种子细胞之一。为了探索MSCs 的 / t: W$ L+ D8 J ]5 t% t迁移和分化趋势, 为帕金森病(Parkinson disease , PD) 的干细胞治疗提供理论和实验依据, 本实验将体外扩增并转染增 0 u- D3 |0 S, I% }" w9 D. p2 `强型绿色荧光蛋白(enhanced green fluorescent protein , EGFP) 的人骨髓MSCs 注入PD 大鼠脑内纹状体, 观察了人骨髓 2 | M- X9 Q5 O5 JMSCs 在大鼠脑内的存活、迁移、分化以及注射MSCs 前后大鼠的行为变化。结果表明, 人骨髓MSCs 在大鼠脑内可存0 r, i. f+ C' K& S/ t
活较长时间(10 周以上) ; 随着时间的延长, MSCs 迁移范围扩大, 分布于纹状体、胼胝体、皮质以及脑内血管壁; 免疫9 A8 v7 M+ X7 g4 E) r+ q+ a
组化法检测证实MSCs 在大鼠脑内表达人神经丝蛋白(neurofilament , NF) 、神经元特异性烯醇化酶(neuron2specific eno2 % }2 `; E& F+ ulase , NSE) 以及胶质原纤维酸性蛋白(glial fibrillary acid protein , GFAP) ; PD 大鼠的异常行为有所缓解, 转圈数由8186 ±. w" G' @: n: {' j0 b3 {2 S
2109 r/ min 下降到4187 ±2106 r/ min , 统计学分析P < 0105 为差异显著。以上观察结果表明, 骨髓MSCs 有望成为治疗. y" V. E6 `$ T8 O' P$ i
PD 的种子细胞。 5 w+ g2 u3 v" i- e9 t$ ~关键词: 骨髓间充质干细胞; 大鼠; 脑; 分化; 迁移 . a" d. O0 M/ S) S8 M+ J8 j中图分类号: Q254 . g; F; r7 _) M2 yMigration and differentiation of human bone marrow mesenchymal stem% q# F$ e& X5 b7 ]
cells in the rat brain 6 _- ~0 j5 W+ U5 A5 `2 a; c# HHOU Ling2Ling1 , ZHENGMin1 , WANG Dong2Mei1 , YUAN Hong2Feng1 , LI Hai2Min1 , CHEN Lin1 ,4 n+ ^3 f0 w$ Q/ A, O" V
BAI Ci2Xian1 , ZHAN G Yong2 , PEI Xue2Tao1 , 3 4 k+ r; m) `: P* p L R7 M1Beijing Instit ute of Transf usion Medicine , Beijing 100850 ; 2 Northwest Sci2Tech University of A gri2 : ^" T g- j+ I* Acul t ure and Forest ry , Yangling , S hanxi 712100 ) ^6 X. m' i9 N. ]# dAbstract : Bone marrow mesenchymal stem cells (MSCs) are multipotent tissue stem cells that can be induced* a( }# V. G2 B+ r. I F5 Q
in vit ro to differentiate into a variety of cells such as osteoblasts , chondrocytes and adipocytes. MSCs are useful 3 K4 R! E K: g1 z + E- p# P# j) \- [, s 4 ~6 Z5 @# A( Zvehicles for both cell and gene therapy for a variety of diseases. Here , we injected human MSCs with enhanced: f2 c. l2 N. D, m9 D! U0 x4 @5 T
green fluorescent protein (EGFP) into the striatum of Parkinson disease (PD) rat and examined their survival ," M% r/ f) J" w' ~7 G: ^( z- e1 Y
migration , differentiation , and the behavior changes in PD rats , which will provide a theoretical foundation and 0 } `! O; N; ~# ]technical method for clinic PD therapy by stem cells. The results showed that human bone marrow MSCs can! f% _; S$ O& r9 K* k1 |
survive in rat brain for a long time (exceeding 70 d) . MSCs were found in multiple areas of the rat brain includ2! F' T& O( f2 N1 C4 v
ing the striatum , the corpus callosum , contralateral cortex and even the brain vascular wall. Immunocytochemical# [: |: [. d. ^$ X4 q2 S5 b
staining suggested that implanted cells expressed human neurofilament (NF) , neuron2specific enolase (NSE) and, `; J7 F; J4 x7 ?" L5 m( T }( u
glial fibrillary acid protein (GFAP) . At the same time , remission in abnormal behavior of the PD rats appeared.3 [; H& E9 o2 v
Rotation scores decreased gradually from 8186 ±2109 r/ min pre2transplantation to 4187 ±2106 r/ min 90 d post2 2 e; D- ~9 e: G* S; v* z; B) i" htransplantation (statistic result showed P < 0105) u( P) G2 O; n1 ^. W1 f: e/ L+ n $ I1 j, X- _/ L附件是pdf全文。感谢大家支持，回复本贴就可以下载了 1 y/ r5 R- s6 i- e( c( h5 h . ~8 f+ r1 c" m7 R[attach]203[/attach]作者: 新人王 时间: 2009-3-10 12:20
