本帖最后由 FYH 于 2010-7-10 00:16 编辑 3 N5 `# R- y) L6 K7 y
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人ES细胞自研究以来，其培养的复杂性是制约其应用的一大方面，先前的培养中，需要铺滋养层或动物来源的基质胶。由于动物细胞与成份的参与带来免疫原性，而且加大ES细胞的自我更新与干性维持机制研究的复杂性，应用的安全性和有效性更加被置疑。而最近的三项研究表明，人ES细胞可以在特定的基质上长期培养，这些研究成果报道在最近同期的nature biotechnology，[Letters, p. 606, 611; BriefCommunications, p. 581; News and Views, p. 562]这为建立ES细胞有效的培养方法迈出坚实一步，在未来的药物筛选与评价，疾病模型的建立与机制研究，甚至是基于其的细胞治疗都有重大意义。（同时附上CELL STEM CELL对此研究的评述。希望对做人ES细胞与iPS细胞的同行有所帮助。, }& N! v& T5 c4 o" d9 m4 X
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Defining hESC culture* K7 F0 c4 X& e( L4 w
Human embryonic( O1 M) \! e( B& D d+ [! l9 Q5 w# v
stem cells (hESCs) in 2 a9 Y+ @& Z4 Yculture—at least in the & z* `: b# p: w) s: p9 n3 Iculture conditions we have & m% z3 P) F1 ^& W6 |( Z/ }today—tend to lose their 5 ?* Q8 W3 K, l, Ndefining characteristic ; K e& J6 R4 c8 S' Tof pluripotency. To9 U) B6 |( t1 l
maintain them in an2 P8 B$ m( Y$ Q. }$ P' ]
undifferentiated state, " q0 X. A6 Y0 L2 `4 Sresearchers often rely$ h( ?7 C0 ~; u+ ^
on complex culture ! S; E; \8 e4 ^3 w3 h. }components such as & Z7 Z( q" v( ?* y9 e8 z7 \mouse ‘feeder’ cells; ~+ L: y0 K8 e+ ?
and Matrigel, an extracellular matrix–like substance derived& u5 {7 j) i& N; q: t
from mouse sarcoma cells. But these materials are xenogeneic, ) X$ U8 D) P2 {9 `5 y# schemically undefined and variable from lot to lot, compromising ) P( U+ l* f/ k. v% k7 K. n: Pthe reproducibility of experimental results and raising safety $ A9 F) m% B" uconcerns over possible contaminants. Feeder cells can already be " O: L% s0 ~: A0 @6 r# Preplaced with fully defined media, but a satisfactory alternative to 9 i7 p0 \% b1 N7 l- \Matrigel remains to be found. Three groups now report fully defined / s) Y: o1 [; {; @substrates that support long-term culture of hESCs. Melkoumian7 }. [1 |6 m" U8 G" i& @6 \
and colleagues show that peptide-acrylate surfaces bearing& r3 u5 C8 Z* E& v
synthetic peptides derived from bone sialoprotein and vitronectin + W# C6 u/ t1 i I2 ?are suitable both for maintaining hESCs and for differentiating 1 O0 |7 t; U3 r8 bthem to cardiomyocytes. Tryggvason and colleagues use a1 @- E, F4 n' |
recombinant form of laminin-511 to culture hESCs and human : v. X8 W" O* L. Zinduced pluripotent stem cells. Finally, Smith and colleagues7 ?$ X5 n7 K/ m8 a2 k! q. P
culture hESCs on a surface made of the synthetic polymer poly[2- : l& n; R4 K) G+ U6 e(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium ' T4 a( o6 S4 @( Shydroxide]. In combination with fully defined media, fully defined 1 @! P/ ~* T8 @substrates should improve reproducibility and safety as well as 0 A% ^' y F/ T* Z& R, o, v/ _facilitate scale-up of hESC production. [Letters, p. 606, 611; Brief. I. d5 D5 p# y4 F7 M% a' n9 g
Communications, p. 581; News and Views, p. 562]作者: 简简单单 时间: 2010-7-10 11:42