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收藏了，谢谢！+ V5 t+ P+ z5 ]5 F' N& }2 L# g 作者: caodainan 时间: 2011-5-5 16:14
这个是什么文献？作者: 懵懂干细胞 时间: 2011-5-5 16:25
Abstract9 }9 H3 g& F6 k$ Q, u Q* X
Introduction Mammary stem cells are bipotential and n8 x+ V3 A) ]( c' {+ `5 f; j; `5 ^% Msuggested to be the origin of breast cancer development, but8 Y. O6 X1 p$ t8 B- \7 y; Y+ q# ?
are elusive and vaguely characterized. Breast tumors can be: U6 G, y& _# [6 U* j5 G; [
divided into subgroups, each one requiring specific treatment. 9 w- ~, y6 C$ a1 J% MTo determine a possible association between mammary stem ( L2 p8 E y4 \! _" F: _0 gcells and breast cancer, a detailed characterization of the5 ]. s8 h# t0 ^7 }! ?* S
transcriptome in mammary stem cells is essential.# E6 e! b: v( Q. l5 X. F3 d
Methods We have used a murine mammary epithelial stem-like 0 g4 r1 p/ u) w1 Z: Z. fcell line (HC11) and made a thorough investigation of global/ m) U5 W/ `9 ]. N( x0 Y- E
gene-expression changes during stepwise differentiation using* Y l; \! j8 ~9 f2 M
dual-color comparative microarray technique. Subsequently, we3 N1 d1 p0 j; c8 t, n' ^
have performed a cross-species comparison to reveal/ z& W; R; ?$ S2 Z( K
conserved gene expression between stem cells and subtype-, U1 W# R8 e6 n5 D
specific and prognosis gene signatures, and correlated gene 9 j: P1 x2 r- {3 P& N) I& bexpression to in vivo mammary gland development. 4 \# @3 n" D1 T" S+ x7 J) r6 nResults Our analysis of mammary stem-like and stepwise cell* `: S6 A6 {9 W/ Y4 j
differentiation, and an in-depth description of our findings in a% k% P7 b* g- Y' W/ n9 X. d, W
breast cancer perspective provide a unique map of the6 v! @8 x8 P" c! m0 Q
transcriptomic changes and a number of novel mammary stem& ] {0 H6 t- }9 M+ J% B+ }
cell markers. We correlate the alterations to in vivo mammary8 H' j5 z; J. S
gland differentiation, and describe novel changes in nuclear : z) q9 v5 f; M2 i! kreceptor gene expression. Interestingly, our comparisons show " s+ J; S. A! O7 h7 Bthat specific subtypes of breast cancers with poor prognosis 5 h) x8 O, w5 e5 k! U& vand metastasizing capabilities show resemblance to stem-like % N, Z0 B+ }, E6 ^7 N4 Cgene expression.* G& K1 f% L4 t0 K" x, V+ @8 l; R
Conclusions The transcriptional characterization of these7 P, K, g, r/ s# A- w
mammary stem-like cells and their differentiation-induced gene+ _/ W1 q. s: D8 w8 Z
expression patterns is here made widely accessible and 0 n8 j4 F/ \! u+ u4 w. D) S" Tprovides a basis for research on mammary stem-like cells. Our8 I5 h9 ~. a( k9 R$ Z9 Z; ?
comparisons suggest that some tumors are more stem-like than6 y, D. D: _, u( }$ l1 U
others, with a corresponding worse prognosis. This information) u/ V7 ?6 f1 {6 ~
would, if established, be important for treatment decisions. We' u3 r' a5 i9 R' b3 |" Y
also suggest several marker candidates valuable to investigate 9 {/ q9 m) K, B1 f$ {, nfurther. 7 t1 v( v$ }9 t( E这是文献的摘要部分！