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标题: 逆转录病毒转染后的问题! [打印本页]

作者: ahfjwangrui    时间: 2010-8-2 09:36     标题: 逆转录病毒转染后的问题!

各位专家,你们好!在做ips细胞时,用逆转录病毒感染靶细胞后,病毒里面的RNA会先在靶细胞里面表达蛋白,还是先整合到靶细胞基因组后再表达相关基因?我们实验室一指导员说是先表达,但我不明白,如果先表达的话,那么细胞里面的RNA酶之类不会将RNA降解吗?这个很疑惑?向大家请教!
作者: Iris    时间: 2010-8-2 10:59

刚进入细胞的病毒RNA是无翻译活性的。病毒借助于自己的蛋白,与细胞融合后,其基因组RNA在细胞质内逆转录,cDNA进入细胞核,在整合酶帮助下整合到宿主DNA,成为前病毒。只有整合的前病毒DNA转录的才有翻译活性。
作者: lixiaodong    时间: 2010-8-2 11:04

RNA模板要反转录成DNA,再整合到genome中。据http://en.wikipedia.org/wiki/HIV
' s" r& j' W. bLentiviruses have many common morphologies and biological properties. Many species are infected by lentiviruses, which are characteristically responsible for long-duration illnesses with a long incubation period.[18] Lentiviruses are transmitted as single-stranded, positive-sense, enveloped RNA viruses. Upon entry of the target cell, the viral RNA genome is converted to double-stranded DNA by a virally encoded reverse transcriptase that is present in the virus particle. This viral DNA is then integrated into the cellular DNA by a virally encoded integrase, along with host cellular co-factors,[19] so that the genome can be transcribed. After the virus has infected the cell, two pathways are possible: either the virus becomes latent and the infected cell continues to function or the virus becomes active and replicates, and a large number of virus particles that can then infect other cells are liberated.




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