标题: 最新综述DNA methylation and cellular reprogramming [打印本页] 作者: shenven 时间: 2010-10-21 14:56 标题: 最新综述DNA methylation and cellular reprogramming
DNA methylation and cellular reprogramming.pdf 3 f6 m8 [/ Y$ f; H: F5 h9 z7 t, |! h3 M8 }# n* p6 j+ L
+ E6 d7 Q$ C6 Q# |
The recent discovery that a small number of defined6 o; n& s, G7 q& h( X
factors are sufficient to reprogram somatic cells into . y& ?9 J. `/ q1 Rpluripotent stem cells has significantly expanded our ; S- D4 {# P, s: X' u- ~2 sknowledge of the plasticity of the epigenome. In this 5 x' M2 O4 E) zreview we discuss some aspects of cell fate plasticity and* `) i+ {& s7 ~: P U9 x
epigenetic alterations, with emphasis on DNA methylation ! n% J" j, ]- K+ j9 tduring cellular reprogramming. Recent data suggest 9 X$ V- {1 Q% w# v- N1 ethat DNA methylation is a major barrier to induced; d- e; |. k$ l6 S& |2 E. K6 g6 F
pluripotent stem (iPS) cell reprogramming. The* _% O* b& J! ^2 v }; m
demethylating agent 5-azacytidine can enhance the efficiency R( f. l" U7 N3 y, _: q+ o: aof iPS cells generation and the putative DNA ) m3 o: K1 C+ e, N$ L- e& l% B; ydemethylase protein activation-induced cytidine deaminase 1 K6 K ^4 T q- S5 V(AID/AICDA) can erase DNA methylation at pluripotency$ K4 H( [/ ^# |- M/ {- O/ G9 ^
gene promoters, thereby allowing cellular' N; p# Y; R) B
reprogramming. Elucidation of the epigenetic changes }$ g( [% R' B$ @taking place during cellular reprogramming will enhance & z) Z1 k' m: dour understanding of stem cell biology and facilitate * T9 r% G- n2 Ntherapeutic applications.作者: swxxx 时间: 2010-10-21 17:54