/ `5 s0 w$ Q8 w. [5 r/ B S不过黄博士坦诚，目前还不清楚这项发现能如何应用于医药治疗。“除了PRDM14，我们目前还在研究很多其他的基因组。当中一些可能对个别病症具重要功能。” 4 d/ h; m6 Y& o. U. t 9 `' Z$ |" P7 [7 `“目前我们很难推算出一个时间表，正如很多重要的治疗，从发现到最终的临床应用是需要时间的。”* h; w F$ G5 g8 G+ j- Q8 B
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虽有人担心，重新编排细胞基因可能会启动不良副作用，但黄博士认为，随着更多关键基因获辨识，重新编排细胞基因的方式也将更完善。- f' ]+ b& d2 }! w6 o
3 b8 Y6 {, u) b$ i+ q, g7 Q黄博士表示，目前一些常见的干细胞疗法用的都是他人捐赠的干细胞，可是新的研究却能直接用病人本身的细胞“培育”出干细胞，仿佛是为病人“量身”制定，相信会更理想。3 ^5 R% Y+ j2 \9 i
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接下来，研究小组将继续探讨如何更好地为普通细胞“添置”PRDM14基因，以及如何利用基因学科技“指引”干细胞“变身”成为个别种类的细胞。 3 n. o( ^$ ~$ G x) z5 o8 v6 F# I' T 8 j6 \" L, [ A7 tNature. 2010 Oct 17. [Epub ahead of print]! I6 ~! ?. n* R5 \* U
. _. s! R) ?) g& x, EA genome-wide RNAi screen reveals determinants of human embryonic stem cell identity. ; D' H q5 m' H% OChia NY, Chan YS, Feng B, Lu X, Orlov YL, Moreau D, Kumar P, Yang L, Jiang J, Lau MS, Huss M, Soh BS, Kraus P, Li P, Lufkin T, Lim B, Clarke ND, Bard F, Ng HH. 0 ~- t4 V! X; s: S) _" s- H8 K y/ ?2 T/ k# D
[1] Gene Regulation Laboratory, Genome Institute of Singapore, 60 Biopolis Street, Singapore 138672 [2] School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551 [3] These authors contributed equally to this work.5 l: M% B$ n( `9 a. e* G
7 \6 Q- `8 ~7 y BAbstract ( E/ j, [' s* |7 u* _! U: }The derivation of human ES cells (hESCs) from human blastocysts represents one of the milestones in stem cell biology. The full potential of hESCs in research and clinical applications requires a detailed understanding of the genetic network that governs the unique properties of hESCs. Here, we report a genome-wide RNA interference screen to identify genes which regulate self-renewal and pluripotency properties in hESCs. Interestingly, functionally distinct complexes involved in transcriptional regulation and chromatin remodelling are among the factors identified in the screen. To understand the roles of these potential regulators of hESCs, we studied transcription factor PRDM14 to gain new insights into its functional roles in the regulation of pluripotency. We showed that PRDM14 regulates directly the expression of key pluripotency gene POU5F1 through its proximal enhancer. Genome-wide location profiling experiments revealed that PRDM14 colocalized extensively with other key transcription factors such as OCT4, NANOG and SOX2, indicating that PRDM14 is integrated into the core transcriptional regulatory network. More importantly, in a gain-of-function assay, we showed that PRDM14 is able to enhance the efficiency of reprogramming of human fibroblasts in conjunction with OCT4, SOX2 and KLF4. Altogether, our study uncovers a wealth of novel hESC regulators wherein PRDM14 exemplifies a key transcription factor required for the maintenance of hESC identity and the reacquisition of pluripotency in human somatic cells. 3 [' i5 D- g# X F 2 E2 u$ p& a* l% r/ j( oPMID: 20953172 [PubMed - as supplied by publisher] 5 |. a3 u2 k4 _7 |! o+ b- f * g5 m8 Z" ~9 p3 U* p2楼原文 感谢zhaoyan1983 提供作者: zhaoyan1983 时间: 2010-10-24 13:03