标题: 求助 Current protocols in immunology 一篇 [打印本页] 作者: woosheng 时间: 2010-10-26 22:28 标题: 求助 Current protocols in immunology 一篇
Curr Protoc Immunol. 2010 Aug;Chapter 14:Unit 14.11.( ]2 P0 t( Q# S Isolation and functional use of human NKT cells. : Y2 A, A8 ?/ g7 A4 KExley MA, Wilson B, Balk SP. K1 C5 y* _; o3 D' z; ]' [ # ~. M. p* Z5 R# U% OCancer Biology Program, Hematology-Oncology Division, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA. : L3 s9 \1 i' v% ^9 L- l% zAbstract" z/ u$ S& E- E& J p$ c A$ h! d
This unit details methods for the isolation, in vitro expansion, and functional characterization of human iNKT cells. The term iNKT derives from the fact that a large fraction of murine NKT cells recognize the MHC class I-like CD1d protein, are CD4+ or CD4-CD8- (double negative), and use an identical "invariant" TCRalpha chain, which is generated by precise Valpha14 and Jalpha281 (now renamed Jalpha18) rearrangements with either no N-region diversity or subsequent trimming to nearly identical amino-acid sequence (hence, 'iNKT'). Basic Protocol 1 and Alternate Protocol 1 use multi-color FACS analysis to identify and quantitate rare iNKT cells from human samples. Basic Protocol 2 describes iNKT cell purification. Alternate Protocol 2 describes a method for high-speed FACS sorting of iNKT cells. Alternate Protocol 3 employs a cell sorting approach to isolate iNKT cell clones. A Support Protocol for secondary stimulation and rapid expansion of iNKT cells is also included. Basic Protocol 3 explains functional analysis of iNKT.8 m* V4 l; k0 g" ]0 {4 I% D5 M
) p5 g. y) b. b% ?! U, qPMID: 20814940作者: laolifeidao999 时间: 2010-10-27 08:16
Isolation and functional use of human NKT cells.[attach]15860[/attach]