作者：赵秀荣，张凤英，赵连志，米术斌，王庆林作者单位：1.承德医学院，河北 承德 067000； 2.湖南师范大学医学院，湖南 长沙 410006 1 {3 S7 n) j. Q, R! w9 k
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【摘要】 目的探讨人巨细胞病毒(human cytomeglavirus,HCMV)对造血干细胞的增殖和分化情况的影响。方法采用造血干细胞体外培养技术,研究HCMV -AD169株对脐血造血干细胞增殖和分化情况的影响,采用PCR技术检测干细胞中的HCMV DNA.。结果HCMV 在体外能明显抑制造血干细胞的增殖和分化,抑制程度随病毒浓度的升高而增大,经PCR检测病毒感染的细胞中有HCMV DNA的存在。结论HCMV-AD169对造血干细胞增殖和分化情况的有抑制作用。 : B, W1 k& m/ E8 k# w
【关键词】巨细胞病毒 造血干细胞 增殖 分化. W6 }" j2 t& _( X
Effect of Cytomeglavirus on Proliferation and Differentiation of Hematopoietic Stem Cell ; r& g% c) W: u2 s 1 r* }7 W! _8 @4 e* i r ZHAO Xiurong， ZHANG Fengying, ZHAO Lianzhi, MI Shubin, WANG Qinglin& M: F) s; G* L" a& E% U
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1.Chengde Medical College, Hebei Chengde 067000,China;9 k- N) R8 K& J
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2. Medical School of Human Narmal University, Changsha 410006, China , g' B# Y: l$ p# m " @1 T1 d! h2 C L/ Z N8 V6 m& D Abstract：ObjectiveTo investigate the effect ofhuman cytomeglavirus（HCMV） on proliferation and differentiation of hematopoietic stem cell（HSC）. MethodsThe technology ofcell culture was used to observe the effect of HCMV-169 strain on proliferation and differentiation of hematopoietic stem cell, and the technology of polymerase chain reaction(PCR) was used to detect the existence of HCMV DNA in HSC. ResultsHCMV AD169 suppressed the proliferation and differentiation of HSC in a dose-dependent manner. HCMV was successfully detected in HSC.ConclusionHCMV AD169 strain can suppress the proliferation and differentiation of HSC.2 @$ \$ {9 X* n" h% p3 W d4 x
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0 H% s0 l# [) \ ［6］ZHAN P,LIU B,LIU WT,et al.Effect of human cytomegalovirus on proliferation of multipotenital hematopoietic progenitors and intervention study in vitro［J］. China Journal of Modern Medicine,2005,15(2)：182.作者: beautylive 时间: 2015-5-27 16:35