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; r6 A- n& n+ D' i& ^! R% m2 Y( f2 oCurrent Protocols in Stem Cell Biology 2007年完整版 1451页 - Z& a# `1 S$ p* i. X, c- t+ C( \) f$ S, z1 U" u
Online ISBN:9780470151808& S0 e4 G* J$ K+ ^1 U
DOI: 10.1002/9780470151808 . q) P8 a3 `3 p5 d/ E/ f ETable of Contents & @% d4 c+ w' S1. Foreword 8 z N: P4 y3 i5 o' m8 e1 {& P3 U2. Preface! P" M) t& E. H% s$ A( B
3. Chapter 1 Embryonic and Extraembryonic Stem Cells/ j/ A. ^' l3 A) {' Y
1. Section A Isolation of Embryonic Stem Cells 2 g0 u$ H. n: u# F; A3 Y9 n2 ]0 T; s1. Introduction ! t- z: `% Z; @9 ]+ [- a, P- I$ @( a$ P2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells 9 g( x9 n$ m q2 G3. Unit 1A.2 Derivation of hESC from Intact Blastocysts( h2 q4 o( Q& i6 G& E
4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells ; ]5 U' ^; G, f8 q" `9 } v5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment ! j% s9 H" b3 r% k: ~. ?2. Section B Characterization of Embryonic Stem Cells . Z. n0 J) L' L8 _0 P% o" x! C1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells 6 ?+ S/ g# m* p; k2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and6 v/ Q/ V1 F/ Y6 F
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform : i0 G5 l5 {* Y3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells! g! f' Y% |8 b I6 {- R. s) a- N% w
4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of6 |/ C5 C& E; Y0 h j/ ]
Pluripotency7 \4 f4 V& Y/ u
5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells; D7 G, V" ~/ m! B$ r
Using In Vivo Biotinylation / C8 t6 i6 T0 h' M8 {$ u6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem$ p: `& g; m$ x* E
Cells) I) r: x! X% U5 }4 H& w- N
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional) R9 d4 |$ Q9 r7 Y, }, N
Profiling ! v9 `( X, A& H1 ]( e7 f# `3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells. t9 o; _$ Y& G6 w% n+ d
1. Introduction/ S) p6 Z% M) i: m7 X! u
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro 1 g5 W/ F( h! I2 X6 r6 J0 l9 R9 r3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture 9 l# R) ~. R8 r4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of . K+ q# x* Z* Y+ N& [# ]Mouse Embryonic Feeder Layer Cells . B6 ]; i0 `1 z3 K* Z |" r5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells; p- Y0 }5 @6 d
6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic * t1 u( |( V; }, E v7 u* ^Stem Cells8 F3 p3 k4 L5 ~4 `! R
7. Unit 1C.6 Isolation of Human Placental Fibroblasts0 K2 @& N1 a( X K
8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic 2 G- M0 F! o/ `5 w2 C, y: GStem Cells 4 Q* G; Z2 [# R/ k$ n9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of6 G6 O5 ]7 E; S6 `" @0 x
ROCK Inhibitor3 N! V' q+ `% O2 i8 R
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells 2 B7 h* }" T9 k11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent8 E8 R2 R* E/ _3 U
Stem Cells on Mouse Embryonic Fibroblast Feeder Cells5 ~9 h" K( D# t- l4 h5 p8 Y
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers$ m. A' F8 [6 L9 Y
4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells " e) M2 [9 [4 b1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap , T: |3 ^; ~5 o& a5 \6 l! \2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by+ v8 d8 `; x3 A i; c$ S
Suspension and Hanging Drop Cultures3 n1 T. ~! j! }3 V3 l# ~* J
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies % B, Z# G8 q$ @0 U1 l f" dand a Description of the Hematopoietic Blast Colony Forming Assay; R5 w. f2 N- H8 i$ ^2 t( W1 B
4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically * a0 D& T) v6 RDefined Culture Conditions. x$ l7 N9 ^- c' F; L$ F( \7 P( u T
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells + Y, R8 @0 |2 L. |+ Q+ E: ~: b5. Section E Extraembryonic Lineages3 e! O9 z+ q3 X7 d; \" U/ ~: r5 N
1. Introduction 4 s# T) r/ B5 |& d2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation3 z+ j E) g+ |& j" ]0 Z
into Hepatocyte-Like Cells3 v! ?( `5 U& T
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta * u N- u0 J+ D' t4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells" |6 e3 y* K+ Y2 b" S5 ?; @' T( T
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells; U) S: D+ {; O9 J u1 u
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells6 t( T2 J( D6 ?1 T _3 k
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use* }$ h' a' P N2 h6 X! ^+ _4 S* D
6. Section F Mesodermal Lineages; o# r9 z0 i4 R9 b# Q) S5 h: Z0 d
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells ' u5 M* b i3 U8 X2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture3 U7 n) b3 m8 P- V6 P9 S
with Endoderm in Serum-Free Medium r x/ C2 C6 r' Q7 R3 ?. }
3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells1 P5 e% f" C* x( G* e6 F; i/ Z
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood ' H2 l" \- x8 N; s+ }5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells1 }/ \4 J. Y! k- |( b
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation ) `+ t: m" b! |1 n+ fwith Stromal Layers1 Q! C" s) f9 F' W7 ?
7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary $ @8 F5 |. P2 J+ z* N3 F6 aMurine Hematopoietic Progenitors# o) w6 r' ~$ l4 H) b
8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from * G8 p+ \" I0 n% X1 [' KHuman Embryonic Stem Cells6 F+ f2 p( A3 h: m
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells 8 ?1 V5 E" e S" Y+ f( O4 H10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac0 D8 O S2 P% F3 J0 d
Progenitor Cells0 T" w$ f7 i/ S7 [
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the $ s* n7 x! | O+ lHanging-Drop and Mass Culture Methods 9 f# S& x; E. |6 J' ^8 w7. Section G Endodermal Lineages ( R' }8 i: ^5 ^6 ?6 v8 i' @1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells ! K* e- C! Z: N! o. ?2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells5 k, Q( }. _3 J! K ]& ?
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm# ? n% D2 [. e F
8. Section H Ectodermal Lineages : H; ]" {; S" o5 O7 ^1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons ! \: I+ K, n! o( {+ M, K2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by! g. k0 Z7 K7 |8 b7 L: K$ x6 ~
Two-Photon Microscopy . H/ s4 k5 a7 O5 K4. Chapter 2 Somatic Stem Cells , r; r7 p( `) K1. Section A Hematopoietic Stem Cells4 r6 ]& y/ p+ i! r& I: l% m, F/ ^
1. Introduction6 ^, v9 X4 L1 O o; i1 Q- h) W$ s
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density6 B% Z+ c* M2 a$ \; u* _: c: q
Gradient % I& K. J! n9 W$ _+ v3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood W( F2 g8 `' E( N' C4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood p8 V6 e6 ^% ^, ~) B Z5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells 1 i E0 ?$ h4 Ofrom Adult Mouse Bone Marrow1 W+ S* E/ U5 m! H% M8 r
6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche; t: ?5 e0 U$ a5 a$ Z
7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine 8 I, V& N B% d5 J5 l( gEmbryonic Tissues. J- ^% U- S2 R4 F5 t
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells# K. e; _6 h% {* P' o" J! F9 a
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells' R( f" G; v4 ~* g7 N: Q; j$ s
10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta3 H! l& m5 |( a/ P5 z
2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells ) ~2 P5 i; \4 d {1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human 8 J7 q! L+ r$ Z5 W) ]1 ATissues8 u2 t( r& P' }6 |
2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells / O/ V& d4 v4 A7 J3 a- o$ i3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells3 m$ Y6 v# e7 A' v
3. Section C Cardiovascular Stem Cells" a8 L5 x5 S- Z
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood 7 U" F) t2 B/ d2 Q$ V2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium $ u3 a; H) p1 Q9 g3 n$ p3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells! W( D( ^1 Q! _1 D9 f3 j* f
4. Section D Neural Stem Cells* l! j! C6 t" `' T
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains! u( i/ C/ a- j6 Y% o' O% d
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells 0 l& A; ]8 r, I; v3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and( B/ N7 C! i9 `& a% H# j
Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal1 ]$ S- C7 V9 ] C8 P
Forebrain Neural Stem Cells5 h( | D0 Q1 c/ G: g) _
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage ' q+ S @5 P+ Z fProgression, and Maturation . {: i: S0 I1 | b# |9 z5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic 8 q8 D* G/ h5 f0 H8 @+ }! DNeurons from Rodent Brains. L! q+ j& \1 n. N( v! o; u' d
6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere 1 D3 n8 a8 D/ D/ {6 G- k, ?% NTechnique ' r# q1 [$ N- F% _0 \: }# L4 a5. Section E Germline Stem Cells % p9 a& u3 T# o1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila4 T; A' s! k* J8 N' |0 q: q! y+ ?6 {& M
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis$ t' U8 i; g0 p: s! a. P
6. Section F Gut Stem Cells* f# J- |8 W6 ]; `
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells 5 f5 K% E i7 `4 M! w7. Section G Lung Stem Cells . N4 S3 ~( O. m+ i: f- _: F9 J1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells # B# o9 c, E; ~4 {6 p% h4 a5. Chapter 3 Cancer Stem Cells1 L( M: Z/ d8 u, |+ K3 V
1. Unit 3.1 Colon Cancer Stem Cells 4 x/ e! v, S3 Z( P# w8 U- j* k2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model & Q6 H7 N4 v. H/ F4 U/ U3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells 0 F( D8 Q2 I3 [ @8 n6. Chapter 4 Manipulation of Potency 7 a& p- r& b2 |! x1. Section A iPS Cells- s+ z9 P: ^1 M8 V
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming / o7 N- ]% F* q$ p, t; E2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells* D7 C4 u6 G9 z( a8 |# J
2. Section B Nuclear Transfer + k) b: ]" E- _1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency ( K1 u: A! G! E$ G- ]7. Chapter 5 Genetic Manipulation of Stem Cells & O) e, b. Y" p! x# l) W1. Section A Lineage Tracers in Stem Cells " c% {3 G* K; ^7 B( A1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma+ E( l; G. _4 J% L* E6 _
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing 8 x4 X# @; q7 ~) b: \+ T% b3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells . F0 T, e+ i' I, P& _! U7 n/ B4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium " m! X- S4 V. N1 l7 ^! n5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian+ r8 }5 {& _, T! J' u2 }
Neocortical Neural Progenitors4 i0 I, V- g* m
2. Section B Homologous Recombination in Stem Cells ' m- Z) B/ S7 W3 _; }* [/ F6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by ; }1 r/ e0 b% g8 J: o2 U9 u/ IHomologous Recombination6 E6 t& O' }: K
8. Appendix 1 Useful Information 7 W# Q$ _9 _, a; P1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research / X/ C( t0 |4 s/ w$ n2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells. q4 b! Y4 H9 O) m+ B- r
9. Appendix 2 Laboratory Equipment9 o+ M4 q( C/ [( W1 M
Standard Laboratory Equipment9 e2 E9 b4 R" p8 {
10. Appendix Suppliers ( a ]0 {9 t# U, _- G$ S* TSelected Suppliers of Reagents and Equipment% _4 E$ W- W4 a& i1 C
# N% B1 W8 n! j. h, Q1 O 作者: angelyannan 时间: 2011-3-8 20:14
