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' m) { s9 E; w7 `5 zCurrent Protocols in Stem Cell Biology 2007年完整版 1451页9 G; n9 m- a6 L/ p/ v
6 S* `# s6 r c- b
Online ISBN:9780470151808' {! q" ^) v$ x
DOI: 10.1002/9780470151808 ) u# Y! f9 k) t$ \Table of Contents 2 |+ o! p+ H: e1. Foreword7 n0 i. Q1 l: H5 A' l" q4 M& f
2. Preface 9 |4 u( Z2 @6 H( F- R" ]$ L0 n/ x3. Chapter 1 Embryonic and Extraembryonic Stem Cells $ L9 \4 s0 k: g' X+ T ?& B1. Section A Isolation of Embryonic Stem Cells$ d o; O7 D6 |6 p' O! Y# m6 k; z
1. Introduction* W7 W4 x! N( d9 [) E
2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells7 O* m0 E* I c6 M6 }6 @. W# Q# p9 ?6 Q
3. Unit 1A.2 Derivation of hESC from Intact Blastocysts $ A: d0 D) R6 ~, D$ a/ }4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells % S5 d! O# u k. H5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment + b, u8 x) g& i/ N2. Section B Characterization of Embryonic Stem Cells1 ~& c) P( Z1 |8 w/ W
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells: {. x3 U! _2 `& Y( M2 Q
2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and5 y/ Q) o1 F4 O6 P# v# D
Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform1 r, C1 K3 s# P) w) _- `+ f+ U y
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells 9 s8 b$ {2 F3 r) t/ m" o4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of - e( F/ i4 f3 P7 ]- H5 }- d4 ^$ T4 aPluripotency - E2 E/ F2 {6 u; G- L5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells % ^- S* C$ E8 s* j! pUsing In Vivo Biotinylation $ C, E3 L1 q- H, Z6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem/ U" l. [7 U; `; C$ w) B( U
Cells, K5 R- N( C7 ~( E* m! g
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional7 N$ R* W7 y( U0 u9 m3 O
Profiling 2 [+ R, e3 A. S/ D3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells( v. ?* v" n; R) u
1. Introduction$ O# O9 D$ x9 k, U
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro " j; d+ u+ q+ p- a- e3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture, u% \+ E. T$ v- T
4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of ' Y7 q9 a: L$ G* e$ EMouse Embryonic Feeder Layer Cells ; Y* A. s- J2 h$ o. S* C5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells % @- G# E' F. s3 A0 O! ^% a6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic " G' a& L" z2 l9 ~% \Stem Cells % K3 m6 t, K$ i: u) x- G7. Unit 1C.6 Isolation of Human Placental Fibroblasts : j- |' g8 y3 u* f8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic4 o# L% c8 b6 q0 X W/ O$ `
Stem Cells( K4 k! W \ V0 E" q
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of% g4 B: M) O9 S) ]' }' u
ROCK Inhibitor% e+ o9 F$ q- t6 A ~
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells' g0 \- |/ L8 l K, _) o
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent * k, X! N& _, T8 r9 QStem Cells on Mouse Embryonic Fibroblast Feeder Cells( j2 b% z' y( [7 V
12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers i+ m, ?- f6 O3 I8 I4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells & ^" r0 `0 C1 g: y* _ d2 q1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap& [, X3 }% g: P% H
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by 1 R0 ^) D: b9 U0 \Suspension and Hanging Drop Cultures, e o; \7 j0 r( B
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies( |4 U9 C1 ` C
and a Description of the Hematopoietic Blast Colony Forming Assay 3 f$ s0 H5 [4 c+ H/ C' A0 N! m4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically6 V1 `8 I- M% _
Defined Culture Conditions; c T9 H6 p# Z" F4 b" Z
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells; B2 L; F4 I6 _0 V
5. Section E Extraembryonic Lineages : n G, n& n6 b6 E6 X9 K1. Introduction 7 F9 A, r6 a! S i2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation) k3 i% G! t3 j4 }; g
into Hepatocyte-Like Cells3 ]2 W# M i9 G
3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta$ R+ U2 b$ r* N3 N; ]" t
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells : {5 U! J* r& _! X4 y5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells - A0 Y y8 [; u1 U# R6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells" f6 i; s+ Z0 m* H, e
7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use* T5 H6 ]% D/ n9 W: D
6. Section F Mesodermal Lineages! h: g9 ^1 S4 J
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells % P; Z; Y5 A. M( `2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture 8 P8 U' W0 X' ]% Pwith Endoderm in Serum-Free Medium $ M" ~ J; A" F7 I3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells8 T `4 F5 z: N2 A
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood% ?/ Z9 i( b# u: H
5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells$ I; t( I- m9 y
6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation1 K4 G$ P( z/ Q! L/ q) W
with Stromal Layers 2 b* ?( L" w, I O( s7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary6 l% @4 u) T9 }4 z+ v$ K) \& W
Murine Hematopoietic Progenitors 4 W/ j% t- s, f: ^8 F8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from ( k$ s. {0 C: QHuman Embryonic Stem Cells3 Y" V! n0 B0 I$ H7 b
9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells # b! h* }+ `! n$ M, p10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac/ d0 }" ~1 |$ P8 V" x& h
Progenitor Cells - i3 q' h+ T1 L. u& p" i- q( ?11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the! ]% P$ ~, }8 I9 h1 d
Hanging-Drop and Mass Culture Methods& r1 k& f- Z0 P! q1 _% t- O
7. Section G Endodermal Lineages0 B, c+ [- h8 w3 |2 X/ s8 k
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells, t4 @" I, t( h
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells $ D& t/ O4 m* K3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm $ _7 A5 f6 r( s% \9 c/ T6 Q3 [- S8. Section H Ectodermal Lineages ( [" \. ~, z9 @/ C7 G1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons 5 p* E; H( c: A2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by 4 n1 p/ H w! _3 p" dTwo-Photon Microscopy $ ]6 D& N4 b: N6 b1 N4. Chapter 2 Somatic Stem Cells9 b1 T; r0 z4 f `' ^: E0 ~. Q1 T2 ~( ~
1. Section A Hematopoietic Stem Cells , C* Z* i1 c) j1 B* `1. Introduction% I; l- ` A( z! f6 \4 T. M1 X+ F# N. H" {
2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density 9 i3 J9 c9 m' lGradient! a i4 k! D2 P9 i( Y: ]$ ^
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood 3 X, Z! d" M$ F# O& _2 e4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood " H1 ?( E+ i+ i' q$ y$ I( v5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells9 R0 `' l$ l: |1 z0 v
from Adult Mouse Bone Marrow ) u: `; l8 ^1 [) c6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche 6 F. d+ m$ G1 H( d. H7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine - t# H' s4 ?1 q9 Q& E P. I7 hEmbryonic Tissues 6 f' t2 L3 F* l+ A3 ]$ J! [8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells! n: I' G. M$ n: D2 n) W( R
9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells ( y _% s3 [9 f6 `+ W' y# I$ p10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta 2 E+ S1 f$ Y/ n1 k6 n7 g8 E2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells: N/ G1 l% [! |6 I4 i
1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human - q2 u& ]" @; ?Tissues ( \: U0 E) p/ s. Y5 {2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells ) l T7 _( X; T& ^9 X+ d: `3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells $ `$ L" r3 P4 L" T1 f/ x- L: P3. Section C Cardiovascular Stem Cells3 F. \# f& D$ i+ ] a: p6 v
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood $ H. I4 x1 W$ a+ N2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium ) M: G+ Q+ y0 }7 r( J8 l) [3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells ' ]1 N9 U+ U0 N! [. [4. Section D Neural Stem Cells 9 ~5 z' k& l1 X! G% k1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains ) ]+ l) o& p) ?& j2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells. f! q! m: @# z* L) a6 o; X9 X6 ?
3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and $ r: Y3 \+ ?' D. q+ iCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal - |: m- I4 H6 f5 a- F3 TForebrain Neural Stem Cells 2 P; q; T, L& |9 B. |' h9 _" S4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage " g. M9 r/ z$ F$ B4 ~7 F! QProgression, and Maturation$ n- \+ H5 S d+ p- }2 A; A1 O1 N
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic 7 C! S' F& b# a2 ?8 }, ZNeurons from Rodent Brains & d. ^9 N. r. S1 x' v1 ^6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere% o( @0 m4 |* G+ _" ^* t' [
Technique8 O5 t+ i- I3 P/ \' B1 f5 G% h
5. Section E Germline Stem Cells' ]2 z I9 d% ?+ Z8 U" B6 r
1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila) s! _6 H0 x" f. ]- R1 n, N
2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis & }3 ]) U4 w* T, d: s4 q) ~6. Section F Gut Stem Cells & k" X5 K2 P2 d; ?$ E6 s) `. n# w1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells , Y$ G9 X' J; s' \' t7. Section G Lung Stem Cells 4 M: ?5 N9 p/ {1 x" B1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells8 W) Z+ h Q( t* h' g* i
5. Chapter 3 Cancer Stem Cells- o, s( v; J" l$ ]# W3 S5 V
1. Unit 3.1 Colon Cancer Stem Cells ! B; U; ~2 q0 w2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model 6 K1 M; ~1 T9 v& Y3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells. t. ^1 L: M. l3 \0 s" l" B
6. Chapter 4 Manipulation of Potency3 z/ R) T) Q' g. V
1. Section A iPS Cells$ _" |7 t# T. g% @7 E
1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming 3 W' P3 [. n, X" D3 B* ]% ?2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells' Z% V9 s. T8 H$ f/ A$ k4 o
2. Section B Nuclear Transfer) ]# I/ \, Z; m$ o6 y
1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency @* ~/ D5 j$ R( R) [0 n1 S5 _/ q% P1 @7. Chapter 5 Genetic Manipulation of Stem Cells9 v9 t0 g" @. A8 n
1. Section A Lineage Tracers in Stem Cells% ~2 c Q5 g- H( R
1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma 1 V! x3 u v" e' q2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing # w" m, Y1 M2 Q$ b$ K$ v/ `3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells9 }) k0 w; _0 w
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium , x. K5 c0 ~9 H: Y- G" d5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian( d- @1 v! h0 Z. j$ d5 O/ [4 w
Neocortical Neural Progenitors 0 ^5 k# M% _0 B; ?; U- E! a6 ]2. Section B Homologous Recombination in Stem Cells ( T# N' G+ V' {3 f8 a1 V9 A6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by" v$ h) @( N7 I" q1 s- ]* r
Homologous Recombination - J' j2 C0 s* M$ e8. Appendix 1 Useful Information* ?2 }/ }" c+ U! b7 O! i
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research* i+ `$ J2 N+ V$ _
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells3 [$ ^. P0 i" e' g+ L# M
9. Appendix 2 Laboratory Equipment + O9 F$ Q3 d( ?0 `Standard Laboratory Equipment* i6 Z: ^$ a7 x: P8 M* E. x
10. Appendix Suppliers ! q. C: q1 z3 cSelected Suppliers of Reagents and Equipment 1 W- [. t7 x9 M- E9 f 6 I, ~; G% q6 b+ G/ _( V作者: angelyannan 时间: 2011-3-8 20:14
