本帖最后由 细胞海洋 于 2014-2-10 21:11 编辑 $ ~3 F- n( c5 d) R$ d) J7 A " {6 R7 @7 q7 T# gCurrent Protocols in Stem Cell Biology 2007年完整版 1451页 / r3 F! h( o6 }9 _. j) d* X 6 v5 d/ N2 `! Q; z( L( KOnline ISBN:9780470151808) c: T3 j* y$ U% ^ |
DOI: 10.1002/9780470151808; s6 p e3 v# Y) H4 C
Table of Contents8 {6 N! L! s7 `8 ]2 Z
1. Foreword9 p! g& K0 Y8 F$ W, s
2. Preface6 H; J2 B3 _- t3 K3 T
3. Chapter 1 Embryonic and Extraembryonic Stem Cells 5 B4 Y9 R" L5 T6 \5 W/ w4 H ?. N( f1. Section A Isolation of Embryonic Stem Cells - B0 k/ @5 x( t6 H8 w" y1. Introduction 1 E) Q; w% v- l/ O- G( i( N2. Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells 3 j8 ^$ P0 r% O; {3. Unit 1A.2 Derivation of hESC from Intact Blastocysts & l! m% [$ B& u5 o0 W' o4 u3 q4. Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells& J7 j' n2 x) ?9 _+ P" C ^' W# N' V
5. Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment . O2 ~/ t* h3 S5 J1 M5 \1 C2. Section B Characterization of Embryonic Stem Cells& M+ B( h- k- f0 g
1. Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells 6 u v5 W0 _8 h% F2. Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and 5 ]/ h D4 @% B- \! y4 g, ^Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform2 a7 _* i# Y' L% ^* [
3. Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells 4 c7 z' w+ l3 X) a; Q6 \4. Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of, m, U! V" C5 ]0 u0 E7 B! V- ^
Pluripotency 0 ^$ R0 J: _3 l2 l5. Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells ( x* C4 C. a) ]7 A" x2 h1 {Using In Vivo Biotinylation: o" y/ \+ k$ h/ v+ @) m, R! f# d
6. Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem4 `# @5 L @* H( ^! L$ p: N6 ~* b0 m
Cells R0 _+ c2 K& x/ `, E+ g3 k- v$ P
7. Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional" a6 E9 z) h7 v+ R* L* S3 H2 L
Profiling' W4 c$ D$ w2 |9 q6 m4 i+ ^
3. Section C Culture and Maintenance of Undifferentiated Embryonic Stem Cells; L3 C4 T- j/ d. p( d7 p9 n2 x1 |9 j% ?
1. Introduction# {6 u# l& r2 e; M# o4 o/ j
2. Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro ( z/ f9 L5 L' W+ V0 w2 f3. Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture " ]- A0 `0 K) z4 N1 h) Z9 J4 v4. Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of B5 {4 R. E1 x& \2 wMouse Embryonic Feeder Layer Cells : s* i* y. `* B5 ^! H# I. ]1 _# l5. Unit 1C.4 Culture of Mouse Embryonic Stem Cells 8 K. O8 F' w& G- J- `1 B6. Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic- ?' |+ v/ J1 r6 [5 V
Stem Cells& o4 ~& N( U2 Z) Z7 {+ u
7. Unit 1C.6 Isolation of Human Placental Fibroblasts ) s7 M$ A' q! i# d8. Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic- G- {4 a0 q$ Q! j+ E, C
Stem Cells: k; D" n# E( {1 X3 R2 g3 y
9. Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of& @9 @" \- o6 e
ROCK Inhibitor6 K. q! v1 j, o" m) H( I" t
10. Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells: X" X- {( o4 Y2 \
11. Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent + S% ]9 J5 W0 b' u! GStem Cells on Mouse Embryonic Fibroblast Feeder Cells - q, q5 Q, j4 x, ~8 T3 M12. Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers 6 _5 S3 r; W! n7 @; i$ a4. Section D Germ Layer Induction/Differentiation of Embryonic Stem Cells3 }% v6 @3 Y. ~* t/ F+ T
1. Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap% C( c" S2 m. @! D# k7 N
2. Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by X& _* `7 r- d. c8 E( e
Suspension and Hanging Drop Cultures5 z$ k( F! b+ r
3. Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies* m- [; ~- N7 {6 `: c$ @( N
and a Description of the Hematopoietic Blast Colony Forming Assay ' O4 @! j8 `% z1 I- B7 K: T4. Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically # S9 {) R. F _' ^$ f0 u$ Y# Z, D7 tDefined Culture Conditions, ^( V/ E" W/ ~* c; T. J
5. Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells 4 h2 v! P- _. K! }8 s& v+ I5. Section E Extraembryonic Lineages 5 E* [. {6 i2 g, z7 Z5 H1. Introduction! a( L. x) h# u2 p8 Y
2. Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation % k6 U+ Z8 {$ }into Hepatocyte-Like Cells ; h: X: q. } t t9 V f" s3. Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta7 h5 j% J! ~, H# p e v
4. Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells$ C/ S* T4 [ v6 U4 G- q
5. Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells; Z; R0 S0 |2 l: R) n# G
6. Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells 0 q5 Z# y6 n2 E# B7. Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use& H& }/ j) I- B h
6. Section F Mesodermal Lineages- z' {' {. P7 b, R9 [
1. Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells3 U3 K P Z, h! [! K, @
2. Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture ' ?) I7 X9 Y8 X* |1 Z+ e# _ l; ewith Endoderm in Serum-Free Medium k1 q3 v; \+ L y3. Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells8 k& |# }* v$ H2 p' g# J# W$ R8 E
4. Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood 0 L% V9 [; W: f6 O& }9 Z) A* j( N/ |5. Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells % d# m" p+ G) q! r0 {6. Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation m' j$ S' _) J+ Z& R% S8 |with Stromal Layers $ f" O& V6 C5 Q, f2 x8 g7. Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary& v8 r l$ {- A3 V& a
Murine Hematopoietic Progenitors ; o* q# ^- ~" w% A" T8. Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from$ a) i9 F$ Y0 J7 P+ ?
Human Embryonic Stem Cells . ^. n' p& F9 o4 S \2 j/ ^; c9. Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells4 R; K7 z3 Z& `/ `$ r
10. Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac- A4 _- m9 B, r: F- Z b4 V
Progenitor Cells, z. C$ m( Z% [. u6 n
11. Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the( m, r; H, R% g
Hanging-Drop and Mass Culture Methods' a% B# |) \! ^2 q; v7 ]
7. Section G Endodermal Lineages+ ?9 x- A- ^* j
1. Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells9 K# U! L, k" L, Q4 }
2. Unit 1G.2 Pancreas Differentiation of Mouse ES Cells5 u, h0 L- V6 Z) _, o0 l
3. Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm 7 d! _+ S X) Z4 ~0 u$ B# d8. Section H Ectodermal Lineages 8 s6 T0 B5 F$ B# t7 @1. Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons . t& F* d% h$ F* F8 A+ g6 a2. Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by, X! ?6 v8 f, ^* b
Two-Photon Microscopy . P4 |9 \/ v( N9 D0 q- j' _4. Chapter 2 Somatic Stem Cells: Q" `# ?7 f6 C L2 e1 S# v7 T1 c- }
1. Section A Hematopoietic Stem Cells+ ^6 U$ F% w) g# c/ ~7 i
1. Introduction + t. K; G, f- t0 p2. Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density- J+ ~1 N7 y: }( R& o; w
Gradient8 [% L7 D) F7 V7 l6 N) a3 F: D
3. Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood& p0 H' m: H% S8 i% e* B
4. Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood ) g% t, [. w {+ i+ z5. Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells8 B. V) |2 J8 G4 G# _& X
from Adult Mouse Bone Marrow 9 i2 W- ]4 @1 {" U. D0 }6. Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche 4 ~$ @6 u" {: o, R9 B7. Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine : a( p0 _3 r9 f4 X: H/ x8 h6 AEmbryonic Tissues3 ?- q" w G+ ?& M: N% Q
8. Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells $ X$ R1 _( I. U" t( }0 J1 L9. Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells ( |' Z5 q: ?: B( W; ^. `; r( x10. Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta 6 z- x/ j- G6 N$ P7 l9 p2. Section B Non-Hematopoietic Bone Marrow-Derived Stem Cells # ?+ J' z' v8 _5 c% H; L" t1. Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human' t3 b6 h1 V |3 m
Tissues # s& [! c4 b4 j4 Y: }2. Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells . K2 Q) w3 y- I r2 y: `9 d6 ~2 O3 f3. Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells: y, c4 J( i; u9 I* ?
3. Section C Cardiovascular Stem Cells; t, v0 A; k3 I) k: d2 I: U1 b' {
1. Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood: p N) j, x8 w
2. Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium ; z# k W, u( e- |6 c4 N/ j D5 y. M3. Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells & `7 j+ K3 e4 u4. Section D Neural Stem Cells, k$ j/ W+ y; f6 z
1. Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains* o2 l7 o. P% h% `* ^4 J
2. Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells ' f/ j% q9 _% {( O8 V7 N: J3. Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and / U+ U4 g( A4 `. M6 t' q* mCharacterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal ( x. E( e6 ]+ @) w0 pForebrain Neural Stem Cells( v/ \4 t4 u& M/ j9 F" R1 Q' ^% B
4. Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage" a4 D! T6 t* W& S) e7 J" I/ \. y( k
Progression, and Maturation7 P* ?- I! m4 L6 m2 \- T& ^
5. Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic ; K9 O @' P0 P+ q1 U* H6 ]; PNeurons from Rodent Brains + @2 t2 b ^! ~6. Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere 4 F3 M# R- C* i- G6 V% E8 uTechnique 3 E( G$ S ^$ a2 T+ ]0 H7 x7 m1 F6 o0 z5. Section E Germline Stem Cells 4 X- Q" w8 ] e+ w+ ]1. Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila S& l/ A6 O/ e5 @* I7 F+ n1 [2. Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis( C; t9 {7 P: F- Q; r+ F ]
6. Section F Gut Stem Cells, u! e9 q& c- Q+ g: Y
1. Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells' q( P" B: L$ J! s5 W2 G
7. Section G Lung Stem Cells2 I% F0 B8 m0 U/ W, k
1. Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem/Progenitor Cells 7 J$ t; Z. v4 r6 E1 X) k5. Chapter 3 Cancer Stem Cells* n& ~( Z* q& l: ]1 N2 y
1. Unit 3.1 Colon Cancer Stem Cells3 t5 y; A, k4 L- M8 Q
2. Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model' T+ g. Z( O X- L
3. Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells 3 [3 `* y- c; @9 g6. Chapter 4 Manipulation of Potency & c! y$ m: s9 R! c/ k1. Section A iPS Cells ( e5 k! _) u8 _5 D I+ d1. Unit 4A.1 Human iPS Cell Derivation/Reprogramming . g" h0 _4 K9 S2. Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells+ L* @5 ~- Z ~6 A
2. Section B Nuclear Transfer j! q3 m$ Z* b9 I1. Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency! R1 o( x3 Q, C" W3 U" i) [
7. Chapter 5 Genetic Manipulation of Stem Cells9 T9 B _9 H, V' p6 b8 r
1. Section A Lineage Tracers in Stem Cells 5 A) @; e, u& t$ V% j, X Y1. Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma% f7 `+ B) _ X* J' b3 D
2. Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing7 I0 v8 f. F- m/ C* W: k
3. Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells9 _4 L- a0 W5 Z# z( n9 {
4. Unit 5A.4 Lineage Tracing in the Intestinal Epithelium/ D! p+ @2 z& v7 p, A, u5 n
5. Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian+ e+ U* r2 \$ x3 w8 ^( o2 G
Neocortical Neural Progenitors * ]& |# }& g" G8 @# Y. F2. Section B Homologous Recombination in Stem Cells % W, E$ }6 p" Z- {; D! |6. Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by ( w% h! s/ |* e5 S# ^Homologous Recombination 4 u5 J. Z2 n9 [2 T& Y8. Appendix 1 Useful Information8 W3 L, D# P' E2 j
1. 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research# Z9 r6 w8 r! x5 J, Y
2. 1B ISSCR Guidelines for the Clinical Translation of Stem Cells - @; O) {: E7 M9. Appendix 2 Laboratory Equipment; t; i' y! c; M
Standard Laboratory Equipment * I: S5 q9 U1 _$ ]/ k; H10. Appendix Suppliers ! Q8 s" v" O8 S) h9 |" YSelected Suppliers of Reagents and Equipment. O% i2 i* ^# c2 X" }! }8 N& F
6 L. P5 _4 p s C3 g作者: angelyannan 时间: 2011-3-8 20:14
