本帖最后由 qianqianlaile 于 2011-3-18 23:29 编辑 5 W- T$ B# G: j8 E" k 3 q8 _: ]; T2 [/ _# B! dMethods Mol Biol. 2010;666:269-84. 8 v3 t; T& I9 T- J9 E7 E% SA method to isolate, purify, and characterize human periodontal ligament stem cells. ( X/ k( D. Y4 C; a& |# eMrozik K, Gronthos S, Shi S, Bartold PM. - {5 A6 }# v2 |6 `& Y) {Mesenchymal Stem Cell Group, Division of Hematology, Institute of Medical and Veterinary Science/Hanson Institute, Adelaide, Australia.( j. w) u: K6 V# B" Q Z: L
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Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) which demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (integrin beta-1), CD44, and CD106 (VCAM-1). Although no single antibody demonstrates specificity for MSCs, isolation based on the expression of individual markers results in homogeneous preparations of PDLSCs. Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast- and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo are also described. / W- x5 ?& l/ y; c; fPMID: 20717790 [PubMed - in process] 2 `( P9 E; }' |- \5 }" I作者: qianqianlaile 时间: 2011-3-19 00:06
本帖最后由 tpwang 于 2011-3-19 09:18 编辑 5 ?" P9 W* v. y+ \4 y" c
" ]0 r. I1 N. i( E; \ s回复 qianqianlaile 的帖子 % p: T* Z0 _: a' z$ E / ] ?6 A3 y2 X不“盐”其烦：* s- M5 R/ J4 C- b
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人牙周膜干细胞群是一种独特的间充质干细胞群，研究证实其在（可直接翻译为“表现出体内形成……的能力”）体内能形成类牙骨质和类牙周膜结构。因此，牙周膜干细胞代表一种有望用于治疗牙周疾病的重建牙周组织的细胞疗法。本研究描述了从人牙周膜组织中分离牙周膜干细胞的两种方法：传统的贴壁法和免疫筛选法，免疫筛选法的原理是牙周膜干细胞表达间充质干细胞相关表面标记物STRO-1抗原、CD146 (MUC-18)、CD29 (整联蛋白 beta-1)、CD44和CD106(VCAM-1)。尽管没有能证明干细胞特性的单一抗体（尽管上述抗体没有表现出MSC特异性），然而根据独特（individuals=各个）标记物的表达来分离可获得同质的（homogenous=均质性）牙周膜干细胞。进一步鉴定了牙周膜干细胞的免疫表型和多能性，结果显示，牙周膜干细胞在体外能分化成脂肪细胞、类成骨细胞和类牙骨质细胞，在体内能形成类牙骨质和类牙周膜样组织。（这是一个很长的被动语态句子，倒过来翻译=“本章”还提供了进一步的方法，用于鉴定牙周膜干细胞免疫表型，及其体外分化为脂肪细胞、类成骨细胞和类牙骨质细胞，体内分化为类牙骨质和类牙周膜样组织的多潜能性。Methods to further characterize the immunophenotype and multipotent capacity of PDLSCs to differentiate into adipocytes, osteoblast- and cementoblast-like cells in vitro, and cementum- and periodontal ligament-like tissues in vivo are also described 3 P# l& t. B& p5 {, b3 W, F5 R2 H* C6 Q% r9 D4 `( ]
这好像不是一个研究，而是一本书的“章节”，见 The present chapter……。/ M( G+ Q0 A5 S# y1 p
) R) L; u! e& X o+ w: K) t 作者: qianqianlaile 时间: 2011-3-19 13:59