我想问一下mir-302 Controls Mesoderm and Endoderm Specification in hESCs这句话中的Specification到底怎样理解啊?还有 miR-302-lefty impairs germ layer specification in differentiating1 j. y. M. W! q2 n6 B, h
中的 specification ?作者: hormone008 时间: 2011-6-5 18:24
specification 如同characteristics. 4 s m; [% n3 a* f与众不同的特点，不用于其他的自身个性作者: qingtinglueshui 时间: 2011-6-5 18:38
呵呵，你能帮我翻译这句话嘛？mir-302 Controls Mesoderm and Endoderm Specification in hESCs作者: qingtinglueshui 时间: 2011-6-5 18:40
还有 miR-302-lefty axis sustains pluripotency marker expression and impairs germ layer specification in differentiating hESC这句话怎么翻译啊？对specification不能正确理解，导致我不能理解很多内容作者: hormone008 时间: 2011-6-5 19:14
mir-302 Controls Mesoderm and Endoderm Specification in hESCs & b* i1 ~1 h% _% e) Z g在胚胎干细胞里，miR - 302控制着中胚层和内胚层的特性或特殊性作者: hormone008 时间: 2011-6-5 19:16
. K. J. r6 t/ @7 BSpecification在发育学中的定义： * W% Z4 U0 F& r, M: H7 b# z. N 2 p7 L5 L6 G2 D3 i% Z3 g4 }The process of commitment can be divided into two stages (Harrison 1933; Slack 1991). The first stage is a labile phase called specification. The fate of a cell or a tissue is said to be specified when it is capable of differentiating autonomously when placed in a neutral environment, such as a petri dish or test tube. (The environment is neutral with respect to the developmental pathway.) At this stage, the commitment is still capable of being reversed. The second stage of commitment is determination. A cell or tissue is said to be determined when it is capable of differentiating autonomously even when placed into another region of the embryo. If it is able to differentiate according to its original fate even under these circumstances, it is assumed that the commitment is irreversible. 3 {; s" F5 s. Y2 b . a2 }# y2 V) Q9 U+ Y; p' n中文翻译为“特化”。意思是在一个中性环境中，细胞或组织能够自动分化（向特定方向）的特性，这时细胞命运仍是可逆的。而如果放在非中性（不同于发育过程的）环境中，细胞或组织仍能自动分化为特定lineage，那就是“决定”了。 - s% Q3 o1 G" d5 b8 {, p7 K5 k/ d" `& r& o
干细胞发育生物学中使用该词举例：( z# b: [2 w0 w" ?0 A8 w1 j
somatic lineage specification3 q' i! }& f/ i! @ o
early specification of the endoderm tissues 9 A* Z. G0 ~: {- iOct4 is essential in the first embryonic lineage specification* O+ l9 a* k" y. r3 K0 g) L
cell fate specifications 8 q: U/ T6 Q' N1 w, {5 VThe specification of the motor neurons into these different columns is controlled, in large part, by genes in the HOX complexes " h+ S8 P4 c9 U+ z) JIntegration of neuronal differentiation with neuronal subtype specification , r( z! q5 c7 C( q, u! J! t" KCell fate specification / Y5 @6 X+ T) G* C* P" E2 [lineage specification in ES cell' T/ ~( w0 P: l3 f- e8 N
Specification of diverse cell types from pluripotent cells is determined by the expression of a precise set of genes, * o9 }) A* {4 E7 ^, C# mdevelopmental specification of HSCs % e! T5 d6 O, B" xCELL SIGNALING REGULATES PE/EPI SPECIFICATION _8 M6 K, u4 ^+ K& w2 \
specification signals such as Activin A to promote hESCs differentiation,# f l, h- U9 y1 H
One of the first events during mammalian development is the formation or specification of the germ cell lineage2 F7 d/ z1 w! R6 U3 j: S