Our electron microscopy showed that although the mouse oocyte; v3 {+ P* Y8 o( }; v* N1 z
had a wide perivitelline space and few short cell junctions on the2 A S6 z# \" D% x+ U/ ?7 W8 x* \8 U
egg surface, the rabbit oocyte showed no perivitelline space but numerous ) P$ R% F( ~3 U# clong gap junctions that projected deeply into the egg cortex.6 D# X* e. u9 P9 a8 ?
As a matter of our experience, it was much more difficult and took 5 x: B- u B/ X6 x# z- m8 ?much longer time pipetting to remove cumulus cells from rabbit than + O% {+ _2 h- _4 Y# nfrom mouse oocytes. Shear stress generated with use of a rotating ( Q6 {; C: e1 a0 ?/ B/ iwall vessel or by pipetting induced stress-activated protein kinase/3 f+ o5 e4 s' m/ H
jun kinase phosphorylation that caused apoptosis in mouse embryos $ E' @8 `- ^& s. B7 X- @0 y(41, 42). It is therefore deduced that because the deep-set junctional 6 K7 s0 V& C, m2 F# W# v# O( ncomplexes firmly anchored the oocyte and corona cells, although the " `; U: ^& @$ Q5 l! M( Wremoval of corona cells damaged plasma membrane of rabbit oocytes,! q9 |4 n% ~" S/ \9 o
leading to their degeneration or lysis, the long-time intensive * B; I( {" h7 ]; ~pipetting during cumulus denudation induced oocyte apoptosis and 7 `; @8 P8 D7 ?2 [inability to mature in vitro. " i) J; z e: w1 P( @6 l请问黄色字幕这两句怎么翻译呢？谢谢 6 @( D' ~9 p: x5 e! |& D+ P' e, I+ b4 Z W" n3 Z8 F2 A 作者: naturalkillerce 时间: 2011-9-22 20:28