香港大学Qilzhou Lian等的最新文章"Functional mesenchymal stem cells derived from human induced pluripotent stem cells attenuate limb ischemia in mice"中宣称： - o6 y- F, l/ R' W: Y- X; n4 z0 M6 u" o' A) [
第一次将人iPS细胞成功诱导为功能性间充质干细胞，将iPSC-MSC种植到小鼠身上发现可以明显改善小鼠后肢严重缺血并促进血管和肌肉再生。 7 K& K1 g3 m* Y1 L6 y 7 X( l$ Q4 X9 W/ q1 CBackground—Aging and aging-related disorders impair the survival and differentiation potential of bone marrow - L. n8 B7 Z* jmesenchymal stem cells (MSCs) and limit their therapeutic efficacy. Induced pluripotent stem cells (iPSCs) may provide" y6 R+ \; Z3 H4 L W1 f
an alternative source of functional MSCs for tissue repair. This study aimed to generate and characterize human 9 X# j- v4 T+ f" f8 A" c% GiPSC-derived MSCs and to investigate their biological function for the treatment of limb ischemia.% J* o* x% ]& U& d" N+ W* d
Methods and Results—Human iPSCs were induced to MSC differentiation with a clinically compliant protocol. Three9 j0 D4 g! L7 L E% ~+ ]
monoclonal, karyotypically stable, and functional MSC-like cultures were successfully isolated using a combination of; j* y: W# Y1 j* B" s0 k
CD24 and CD105 sorting. They did not express pluripotent-associated markers but displayed MSC surface antigens 5 v: x, M9 f) k. v2 k8 t8 ]and differentiated into adipocytes, osteocytes, and chondrocytes. Transplanting iPSC-MSCs into mice significantly 7 I! }$ S Y: P; {2 `! \attenuated severe hind-limb ischemia and promoted vascular and muscle regeneration. The benefits of iPSC-MSCs on l& ]9 B+ D2 d; o9 U- B2 g
limb ischemia were superior to those of adult bone marrow MSCs. The greater potential of iPSC-MSCs may be 3 F5 O5 [$ x7 ]attributable to their superior survival and engraftment after transplantation to induce vascular and muscle regeneration6 u5 m9 o% {' J+ r% P0 m5 l
via direct de novo differentiation and paracrine mechanisms. ! f! N$ B2 A& K2 YConclusions—Functional MSCs can be clonally generated, beginning at a single-cell level, from human iPSCs.4 e; d9 e2 p s+ e/ V
Patient-specific iPSC-MSCs can be prepared as an “off-the-shelf” format for the treatment of tissue ischemia. " L' |* u7 Z8 s(Circulation. 2010;121:1113-1123.)" s. Z- ^5 X8 G& B! U
Key Words: induced pluripotent stem cells ischemia mesenchymal stem cells peripheral vascular disease ; z2 m; D" [9 ~( a1 v: [ repair transplantation作者: amywhere 时间: 2012-3-27 10:03