是的 Rules: & V) j6 Y4 U4 ^5 N1. Amplicon size should be ranged from 90bp to 180bp;, f8 \; v+ ]6 `( q; K2 `
2. Oligo length should be ranged from 17 to 20bp, normally; 1 T9 P2 T( N- ^9 X3. Ideally, the TM value should be 60℃, but 59℃ to 61℃ is acceptable; 7 b: t! Q6 H; G; {5 f, o4. The TM value of one pair primers should not be different at more than 1℃ ' }% n: ]' L: S# E# Y5 _. l# i' U. ?; n' w5. GC content should be 45% to 55%" f; t* }; y% l( q' `
6. The oligo designed should be located in different exons. 3 J5 h# o4 H' @$ K4 r作者: 不倒翁 时间: 2012-5-29 12:40
是的 Rules:; A# ?4 P" Z3 Z/ y0 Z8 p
1. Amplicon size should be ranged from 90bp to 180bp;: G- H; }% T* w. r' `) @
2. Oligo length should be ranged from 17 to 20bp, normally; ( F4 L3 e: Y" K, O) y4 Z3. Ideally, the TM value should be 60℃, but 59℃ to 61℃ is acceptable; & f# e0 G# G: r5 V* j1 b/ V4. The TM value of one pair primers should not be different at more than 1℃ . Q2 I! t0 l4 t5. GC content should be 45% to 55% ! E' @0 G# p- ]0 B R6. The oligo designed should be located in different exons.3 W8 T5 ]" u& _8 B& ?- A 作者: miRNA 时间: 2012-5-29 13:48