标题: The conserved Pkh–Ypk kinase cascade is required for endocytosis in ye [打印本页] 作者: 杨柳 时间: 2009-3-6 00:06 标题: The conserved Pkh–Ypk kinase cascade is required for endocytosis in ye
Department of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL, 60208! T/ b! \. j+ r0 n1 W6 e& v
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Address correspondence to Linda Hicke, Dept. of Biochemistry, Molecular Biology, and Cell Biology, Northwestern University, Evanston, IL 60208. Tel.: (847) 467-4490. Fax: (847) 467-1380. E-mail: l-hicke@northwestern.edu9 A4 ^5 ^' h. C- b8 J! y
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Abstract0 W% u. x; |$ w* Z
( ]& ^- Z+ x7 Y! |% k6 B8 ?* XInternalization of activated signaling receptors by endocytosis is one way cells downregulate extracellular signals. Like many signaling receptors, the yeast -factor pheromone receptor is downregulated by hyperphosphorylation, ubiquitination, and subsequent internalization and degradation in the lysosome-like vacuole. In a screen to detect proteins involved in ubiquitin-dependent receptor internalization, we identified the sphingoid base–regulated serine–threonine kinase Ypk1. Ypk1 is a homologue of the mammalian serum– and glucocorticoid-induced kinase, SGK, which can substitute for Ypk1 function in yeast. The kinase activity of Ypk1 is required for receptor endocytosis because mutations in two residues important for its catalytic activity cause a severe defect in -factor internalization. Ypk1 is required for both receptor-mediated and fluid-phase endocytosis, and is not necessary for receptor phosphorylation or ubiquitination. Ypk1 itself is phosphorylated by Pkh kinases, homologues of mammalian PDK1. The threonine in Ypk1 that is phosphorylated by Pkh1 is required for efficient endocytosis, and pkh mutant cells are defective in -factor internalization and fluid-phase endocytosis. These observations demonstrate that Ypk1 acts downstream of the Pkh kinases to control endocytosis by phosphorylating components of the endocytic machinery.& C7 A2 O* W5 p% g1 P7 g# d, V
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Key Words: serinethreonine kinase; sphingolipid; receptor downregulation; endocytosis; internalization 5 g" K2 a& ]; c 1 D T; R( j6 W7 ^4 P! W' hIntroduction5 o$ ^) K: B8 u! `( x* M
( M$ q8 b1 q+ D5 L/ U0 ]% oThe internalization of plasma membrane proteins into the endocytic pathway is a key regulatory event in signal transduction, nutrient uptake, and ion homeostasis. The activity and interaction of the network of proteins and lipids that comprise the endocytic machinery is carefully controlled in response to intra- and extracellular signals. This control is exerted by many types of regulatory proteins, including protein and lipid kinases, phosphatases, GTPases, and proteins that regulate actin dynamics. Lipid messengers, such as phosphoinositides, sphingolipids, and sterols, also play key roles in endocytosis (for review see D'Hondt et al., 2000). Sphingolipids and their precursors, sphingoid bases and ceramides, have recently emerged as important but poorly understood activators of endocytosis. Exogenous addition of C6 ceramides or liberation of ceramide from sphingomyelin by addition of sphingomyelinase promotes endocytic vesicle formation in mammalian cells (Zha et al., 1998; Li et al., 1999). In yeast, an lcb1 (long chain base) mutant defective in the first step of sphingolipid synthesis was isolated in a screen for endocytosis mutants, and the lcb1 endocytic phenotype is rescued by exogenous addition of sphingoid bases (Munn and Riezman, 1994; Zanolari et al., 2000).; E; k' u: G2 r, K) L/ X, q( f) p& b
- t) h' O: Y& o5 H* ^, e* P! gCeramide and sphingoid bases may be crucial for endocytosis because they activate regulatory phosphorylation cascades. Inactivation of protein phosphatase 2A, or overexpression of either the Pkc1 or Yck2 kinase, suppresses the endocytosis defects of an lcb1 mutant, suggesting that sphingoid base–stimulated kinase activity is important for receptor endocytosis (Friant et al., 2000). Endocytic proteins that are kinase substrates include clathrin (Wilde et al., 1999), amphiphysin (Bauerfeind et al., 1997), dynamin (Robinson et al., 1993), synaptojanin (McPherson et al., 1994), Eps15 (Fazioli et al., 1993), and epsin (Chen et al., 1999). The regulated phosphorylation of these proteins is likely to be critical for the assembly and disassembly of the network required for internalization (Slepnev et al., 1998). & x2 K9 e+ u- W+ s. z; X% ~: l" l4 _* q
Many of the proteins comprising the internalization machinery are conserved from yeast to mammals, and yeast has been exploited to identify novel proteins that participate in receptor internalization (for review see D'Hondt et al., 2000). Receptor-mediated endocytosis has been studied in Saccharomyces cerevisiae using Ste2, a G protein–coupled signaling receptor that is rapidly internalized in response to binding its ligand, -factor (Jenness and Spatrick, 1986). The isolation of mutants defective in Ste2 internalization has revealed a novel role for the sphingoid base–regulated Pkh and Ypk kinases in the internalization step of endocytosis. ( k1 [ F; O I& o- l7 g. T - J" N1 B; I% k0 m, b- ]; o) ~" t; QResults and discussion: u! M- L5 p$ f% c6 h! e- t: L6 M
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Ypk1 is required for endocytosis6 F, _3 U8 I2 R0 g& M" A+ B
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Ubiquitination of the Ste2 cytoplasmic tail is required before internalization (Hicke and Riezman, 1996). We performed a screen of ethyl methanesulfonate–mutagenized cells to identify novel proteins involved in ubiquitin-dependent receptor internalization. One mutant, udi5–1 (ubiquitin-dependent internalization), that was defective in -factor internalization at both 24°C and 37°C (Fig. 1 A), showed reduced growth on YPUAD 2 mM EGTA. We screened a genomic DNA library for plasmids that rescued this growth defect and identified a plasmid carrying the YPK1 gene. A centromere-based plasmid carrying YPK1 restored the ability of udi5–1 both to grow on YPUAD 2 mM EGTA (unpublished data) and to internalize -factor (Fig. 1 A). A ypk1 strain had an internalization defect similar to the udi5–1 strain (Fig. 1 B), suggesting that the mutation in the udi5–1 strain was in YPK1. We rescued the ypk1 gene from udi5–1 cells, and found that it had a single point mutation in the coding region for the Ypk1 catalytic domain that changed glycine 490 to arginine. Expression of Ypk1G490R in ypk1 cells did not rescue internalization, whereas expression of Ypk1 did (unpublished data). These results demonstrate that UDI5 is allelic to YPK1, and hereafter we refer to udi5–1 as ypk1G490R.6 v" G1 n! h2 S5 w ^
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Figure 1. Ypk1 is required for -factor internalization. (A and B) Internalization of 35S--factor was measured by the continuous presence protocol at 37°C (except where noted) after growth in YPUAD. ypk1G490R cells are the same as udi5–1 cells. (A) YPK1 (LHY291, ?); ypk1G490R (LHY2543 ); ypk1G490R with pYPK1, a centromeric plasmid (LHY2712, ); ypk1G490R (LHY2543, , at 24°C). (B) YPK1 YPK2 (LHY2632, ?); ypk1 YPK2 (LHY2536, ); YPK1 ypk2 cells (LHY2633, ). (C) Schematic diagrams of Ypk1, Ypk2 (68% identical to Ypk1), and human SGK (50% identical to Ypk1). Residues mutated in this study and their counterparts in Ypk1 homologues are shown. The percent identity of the kinase domains is shown in the gray box. Phosphorylation sites are indicated with an arrow with known kinases noted. ' X* n9 ?; L& i9 ?1 [ ! M- o& B/ M/ g8 J# E* @5 W- aYpk1 is a serine–threonine kinase involved in sphingolipid signaling (Sun et al., 2000). Ypk1 has a S. cerevisiae homologue, Ypk2 (68% identical), and a mammalian homologue, SGK (50% identical) (Casamayor et al., 1999) (Fig. 1 C). The amino acid mutated in ypk1G490R cells, G490, is conserved in Ypk1 homologues. Unlike ypk1 cells, ypk2 cells exhibited no -factor internalization defect (Fig. 1 B). Ypk1 is important for endocytosis and cannot be replaced by Ypk2, despite their high degree of conservation, whereas Ypk2 plays either no role in endocytosis or a role that can be fully assumed by Ypk1. Ypk1 and Ypk2 perform at least one redundant, essential function because ypk1 ypk2 cells are dead, even though each single-null mutant is viable (Chen et al., 1993; Casamayor et al., 1999). ypk1G490R ypk2 cells are also dead, suggesting that the Ypk1G490R protein is completely inactive. , |0 z0 Y' N3 e6 h1 L$ x& M2 n) s& Q : ?2 A I; C0 X1 Q7 \To investigate the role of Ypk1 in fluid-phase endocytosis, we assayed the ability of ypk1 cells to deliver Lucifer yellow (LY)* to the vacuole (Fig. 2, A and B). Both ypk1G490R and ypk1 cells were significantly impaired in LY transport compared with their congenic wild-type strains. Ypk1 was also required for internalization of receptors carrying the linear peptide internalization signal NPFXD (Tan et al., 1996), instead of a ubiquitin signal (unpublished data). Ypk1 is not generally required for membrane trafficking, because carboxypeptidase Y was transported through the biosynthetic pathway to the vacuole with normal kinetics in ypk1G490R cells incubated at the restrictive temperature (unpublished data). These observations indicate that Ypk1 is necessary for fluid-phase endocytosis and for the internalization of plasma membrane proteins carrying different types of internalization signals. - n: |% ]/ X5 s6 S: N4 b. U0 K: T + x" d: j. ~5 o9 ]" JFigure 2. Ypk1 is required for fluid-phase endocytosis. (A and B) Lucifer yellow (LY) localization was assayed in ypk1G490R (LHY2543), ypk1 (LHY2536), and wild-type cells from the same tetrad as each mutant (LHY2761 and LHY2537, respectively). Cells were grown to early logarithmic phase in rich medium at 24°C, shifted to 37°C for 15 min, and then allowed to internalize LY at 37°C for 30 or 60 min (A) Images were taken using DIC optics (top) and fluorescence optics (bottom). (B) The percent of cells that accumulated LY in their vacuoles was quantified for the 30- and 60-min time points by blind counting of each sample. The number of cells counted is indicated to the right of the corresponding bar./ u* b1 }6 D5 U* M2 }
0 o. ]4 d+ S9 J/ Z# W6 GThe kinase activity of Ypk1 is required for endocytosis downstream of receptor phosphorylation and ubiquitination 5 v! Q" C* g% k* B3 k9 s9 U4 O+ {) [
To test whether the kinase activity of Ypk1 is required for its function in internalization, we made two mutant forms of epitope-tagged Ypk1 that abolish its kinase activity, Ypk1K376R and Ypk1D488A (Casamayor et al., 1999; Sun et al., 2000). We assayed cells expressing similar levels of the different Ypk proteins (Fig. 3 A) for their ability to internalize -factor. Both ypk1K376R and ypk1D488A mutants showed internalization defects similar to that of ypk1 (Fig. 3 B), demonstrating that the kinase activity of Ypk1 is required for its role in endocytosis.6 j( S- h8 S G4 W2 m
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Figure 3. The kinase activity of Ypk1 is required for receptor internalization, but not for receptor modifications. (A) Extracts of ypk1 cells expressing hemagglutinin (HA)-tagged wild-type Ypk1, Ypk1K376R, or Ypk1D488A were subjected to immunoblotting with -HA antibodies. (B and C) Cells were grown and assayed for -factor internalization as in Fig. 1. (B) The same cells used in A: ypk1 (LHY2536, ?); YPK1 (LHY2563, ); ypk1K376R (LHY2564, ); ypk1D488A (LHY2565, ). (C) YPK1 cells expressing Ste2–378Stop (LHY825, ?) or Ste2-Ub (LHY558, ); ypk1G490R cells expressing Ste2–378Stop (LHY2684 ) or Ste2-Ub (LHY2690 ). (D) ste2 (LHY10), ypk1G490R (LHY2543), or YPK1 (LHY1) cells were treated ( ) or not (-) with 1 μM -factor for 8 min at 37°C. Cell lysates were resolved by SDS-PAGE, transferred to nitrocellulose, and probed with -Ste2 antibodies. Phosphorylated (P) and monoubiquitinated (Ub) species are denoted with arrows. , q8 F. l* S! K- F7 p4 V9 |+ s
We used two approaches to test whether Ypk1 is involved in Ste2 phosphorylation, which is a prerequisite to receptor ubiquitination and internalization (Hicke et al., 1998). First, we assayed -factor internalization by a Ste2-Ub chimeric protein that does not require posttranslational ubiquitination for endocytosis (Terrell et al., 1998; Dunn and Hicke, 2001). In ypk1G490R cells, internalization of Ste2-Ub was as defective as the internalization of a similar receptor carrying posttranslational ubiquitination sites but no fused ubiquitin (Ste2–378Stop) (Fig. 3 C), consistent with the idea that Ypk1 functions after receptor ubiquitination. Second, we demonstrated that Ste2 was phosphorylated and ubiquitinated normally in ypk1G490R and ypk1 cells (Fig. 3 D; unpublished data). The modified forms accumulated in ypk1G490R cells when compared with wild-type cells, as observed in other endocytic mutants (Hicke and Riezman, 1996). These results indicate that the kinase activity of Ypk1 is required for receptor internalization, and that Ypk1 is not involved in ligand-stimulated receptor modifications.7 S! ]1 | u7 q( L
. Q! D$ E2 L% Y% C' x5 nPkh-dependent phosphorylation of Ypk1 is required for efficient internalization) A! E& o* v9 v" A0 t U3 y B
+ y% N. R0 X6 l$ O1 BYpk1 contains two conserved phosphorylation sites, T504 and T662 (Fig. 1 C). T504 is phosphorylated by Pkh1, a yeast homologue of the phosphoinositide-dependent kinase, PDK1 (Casamayor et al., 1999; Inagaki et al., 1999). To determine if either T504 or T662 is involved in the endocytic function of Ypk1, we mutated these residues to alanine alone or in combination. We integrated the constructs into ypk1 cells, identified transformants with equivalent expression levels of epitope-tagged Ypk1 variants (Fig. 4 A), and performed -factor internalization assays on these cells. Cells expressing Ypk1T662A showed a slight defect in internalization, whereas the cells expressing Ypk1T504A or Ypk1T504A,T662A were more strongly impaired (Fig. 4 B). These results indicate that the conserved phosphorylation sites of Ypk1 are required for efficient internalization. T504 appears to play a critical role in internalization, suggesting that phosphorylation by Pkh1 is important for endocytosis. The modest but significant internalization defect observed with cells expressing Ypk1T662A suggests Ypk1 endocytic activity may also be regulated by phosphorylation at T662.7 N% v/ D, T% F- T
; r( G; L6 Y9 T5 Q4 J. M( S, xFigure 4. The conserved phosphorylation sites of Ypk1 are required for -factor internalization. (A) Extracts of ypk1 cells expressing HA-tagged wild-type Ypk1, Ypk1T504A, Ypk1T662A, or Ypk1T504A,T662A . (B) The same cells used in A were grown and assayed as in Fig. 1: ypk1 (LHY2536, ); YPK1 (LHY2563, ); ypk1T504A (LHY2568 X); ypk1T662A (LHY2567, ); ypk1T504A,T662A (LHY2569, ).; _( P+ N4 N! ]3 D- }
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Due to the importance of T504, we investigated the role of Pkh1 in endocytosis. Pkh1 shares significant homology with two other yeast kinases, Pkh2 and Pkh3. Functions of these kinases are at least partially redundant because null mutations in individual PKH genes are not lethal, whereas a pkh1 pkh2 mutant is dead or slow growing (Casamayor et al., 1999; Inagaki et al., 1999; see below). We tested the ability of pkh1, pkh2, and pkh3 cells to internalize -factor and deliver LY to the vacuole. None of the single pkh mutants showed a defect in either assay as compared with isogenic wild-type cells (unpublished data). We then created double mutants to examine if Pkh kinases function redundantly in internalization. It has been reported that cells carrying a deletion of both PKH1 and PKH2 are inviable (Casamayor et al., 1999; Inagaki et al., 1999). In our genetic background, pkh1 pkh2 cells were viable, but grew slowly at 24°C (Fig. 5 A). We found that the slow growth phenotype of pkh1 pkh2 cells could be suppressed substantially by 1.2 M sorbitol, even at 30°C (Inagaki et al., 1999; Fig. 5 A). pkh1 pkh2 cells grown in sorbitol displayed a strong defect in -factor internalization and in accumulation of LY in the vacuole (Fig. 5, B and C). By contrast, pkh1 pkh2 cells grown in sorbitol were not generally defective in protein transport processes because they efficiently transported carboxypeptidase Y to the vacuole (unpublished data) and they appeared morphologically normal (Fig. 5 C). To support these findings, we obtained a mutant strain that is temperature sensitive for Pkh function (pkh1D398G pkh2) (Inagaki et al., 1999), but which grows normally at 24°C (Fig. 5 A). The pkh1ts strain was severely defective for -factor internalization at the nonpermissive growth temperature of 30°C (Fig. 5 D). Like ypk1 mutants, pkh mutants showed no defect in Ste2 phosphorylation or ubiquitination (unpublished data). These results indicate that the Pkh family of kinases is required for endocytosis, and suggest that at least one of their roles is to activate Ypk1 by phosphorylating T504.; I' |0 F- o) `3 P( [
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Figure 5. Pkh kinases are required for receptor-mediated and fluid-phase endocytosis. (A) Growth of pkh1 pkh2 cells and pkh1 pkh2 leu2::PKH1 cells from the same tetrad (LHY2716 and LHY2714, respectively) or pkhts (pkh1D398G pkh2) cells (LHY3030) and an isogenic pkh2 strain (LHY3032) on YPUAD at 24°C or 30°C or on YPUAD 1.2 M sorbitol at 30°C after 4 d. The differences in strain background between the two pkh mutants are likely to account for the difference in suppression of the growth defect on sorbitol medium. (B) The same pkh1 pkh2 () and pkh1 pkh2 leu2::PKH1 () strains as in (A) were grown overnight in YPUAD 1.2M sorbitol. Internalization of 35S--factor was measured by the continuous presence protocol at 30°C in YPUAD 1.2 M sorbitol. (C) LY localization was assayed for wild-type cells (LHY2762), pkh1 cells (LHY2759), pkh2 cells (LHY2760), pkh1 pkh2 cells (LHY2716), and end4 cells (LHY37). Cells were grown to early logarithmic phase in YPUAD 1.2 M sorbitol at 24°C, shifted to 30°C for 15 min, and then incubated with LY at 30°C for 60 min. Images were taken using DIC optics (top) and fluorescence optics (bottom). The pkh1 pkh2 cells that are brightly stained throughout the whole cell are probably lysed cells. (D) pkh1 cells (LHY3031, ?), pkh2 cells (LHY3032, ), and pkhts cells (LHY3030, ) were grown overnight in YPUAD. Internalization of 35S--factor was measured by the continuous presence protocol at 30°C in YPUAD. * n& k, ]- f* D7 L; k( ?3 G0 ?9 n3 j: \$ g# @6 R4 L
A conserved, sphingoid base-regulated kinase cascade in endocytosis2 h8 E5 J ]1 k) K* R5 d% ?
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Sphingoid bases are required for the internalization step of endocytosis in yeast. A lcb1–100 strain defective in sphingolipid biosynthesis is defective in receptor and fluid-phase endocytosis, and the addition of sphingoid bases suppresses this defect (Munn and Riezman, 1994; Zanolari et al., 2000). In addition, our screen identified a lcb2 mutant strain with an -factor internalization defect similar to that of lcb1–100 (unpublished data). Pkh and Ypk kinases are two members of a kinase cascade that function downstream of sphingoid bases. Ypk1 was isolated as a suppressor of growth inhibition by ISP-1, a toxic compound that acts by depleting intracellular sphingoid base levels. Furthermore, phosphorylation of Ypk1, which is required for its kinase activity, is dependent on the level of sphingoid bases in cells (Sun et al., 2000). ypk1 mutants are synthetically lethal with both lcb1 and lcb2 mutants (unpublished data), an observation that supports a connection between sphingoid base synthesis and Ypk activity. Unlike the Yck and Pkc kinases, overexpression of Ypk1 does not suppress the internalization defect of lcb1 or lcb2 (Friant et al., 2000; unpublished data). This may be because overexpression does not increase the level of active, phosphorylated Ypk1, or because sphingoid bases regulate other endocytic proteins whose activity is not increased in a Ypk1-overexpressing cell. g% [( }' x+ y# H2 b5 R2 V, X$ Z! D L% w
Pkh kinases are likely to be the link between sphingoid bases and Ypk1 activation. Pkh-dependent phosphorylation is required for Ypk activity and the overexpression of Pkh1, like Ypk1, suppresses growth inhibition by ISP-1 (Sun et al., 2000). PDK1, the functional mammalian homologue of Pkh1 (Casamayor et al., 1999), is activated by sphingoid bases (King et al., 2000). Like Pkh1, Ypk1 has a mammalian homologue and the Pkh–Ypk kinase cascade is conserved in mammalian cells. PDK1 phosphorylates SGK at T256, a position equivalent to T504 in Ypk1 (Fig. 1 A) (Park et al., 1999). Although a role for PDK or SGK in endocytosis has not been reported, another Ypk homologue, PKB/Akt, is required to activate a small GTPase, Rab5, that is involved in endocytic trafficking (Barbieri et al., 1998). $ i/ R! @( u! F" R% w7 ^7 L1 b) x. Q9 `; e! R/ f
Sphingoid base–dependent phosphorylation may regulate Ypk1 by controlling its localization. Treatment of yeast cells with sphingoid bases appears to cause increased association of overexpressed GFP-Ypk1 with the plasma membrane (Sun et al., 2000); however, we did not see this effect using subcellular fractionation of Ypk1 expressed at normal levels. Cellular substrates of the Ypk kinases have not been reported. Friant et al. (2000) suggested that a target of sphingoid base regulation may be an actin-binding protein because the overexpression of sphingoid bases suppresses both the endocytic and the actin cytoskeleton defects in a lcb1 mutant. One of the many proteins that link actin to endocytosis in yeast may be regulated by Ypk1-dependent phosphorylation. 9 G9 }: B7 @$ F( e + n' P" O3 T8 i" IRegulation of the internalization step of endocytosis requires multiple kinases that receive input from different sources for efficient assembly of endocytic vesicles. We have shown that the Pkh–Ypk1 kinase cascade is an important regulatory component of the endocytic machinery.9 x4 e- b0 x* H7 ?; L/ L x; B
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Materials and methods * w! X: ^& k. h+ S + D, p4 W; @, i- D* g3 K) kReagents ! t! _) r' ?7 t/ g) k; o; {, e 6 N/ Q/ e/ t( `. l! ~All strains were grown in minimal (SD) or rich (YPUAD) medium (Sherman, 1991) as indicated in the figure legends. Table I lists the strains used in this study. BY4741 strains containing deletions in YPK1, YPK2, PKH1, PKH2, and PKH3 were obtained from EUROSCARF. pkh1ts pkh2 and related strains were a gift from Kunihiro Matsumoto (Nagoya University, Nagoya, Japan). bar1 derivatives were generated by single-step gene transplacement or by crossing to a bar1 strain. Ste2 antiserum and 35S--factor were purified as previously described (Dulic et al., 1991; Hicke and Riezman, 1996). HA monoclonal antibody 12CA5 was a gift of Robert Lamb (Northwestern University, Evanston, IL). 8 }1 T8 d! S' p& J$ \3 o, J1 V0 P' |. O5 N
Table I. Strains used in this study 2 \3 a! p. e; R7 e5 M# ?/ i0 n/ ]9 Z" X+ h# I# {, v7 G, L
STE2 plasmids used in this study have been described (Terrell et al., 1998; Dunn and Hicke, 2001). A triple HA-epitope was inserted in-frame at the NH2 terminus of Ypk1 in pRS303 (LHP1113). The HA-YPK1 plasmid rescued the growth and internalization defects of ypk1G490R cells. Mutations in LHP1113 were generated using site-directed mutagenesis (QuickChangeTM; Stratagene). 6 s1 V8 I% M: F8 ?& e O- A* I2 n% c1 a. `' E
Screen for udi mutants! F- t7 y8 ~9 A
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LHY1451, which expressed a variant -factor receptor with only ubiquitin-dependent internalization signals (Ste2–378Stop), was treated with 3% ethyl methanesulfonate for 90 min. Mutagenized cells were tested for their ability to internalize -factor using an assay modified from the method of Dulic et al. (1991). Cells were grown in YPUAD to mid logarithmic phase, then incubated at 37°C for 15 min before the addition of 35S--factor. 20 min after -factor addition, aliquots of cells were placed in either pH1 or pH6 buffer and cell associated radioactivity in each sample was determined as described (Dulic et al., 1991). Under these conditions, LHY1451 cells internalized 68 ± 8% of the surface-bound -factor. Mutagenized cells that consistently internalized 6 H, c) n2 L G" Y
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Cloning of YPK1$ U" v: v% z1 F. k" R/ Y
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YPK1 was cloned from a centromeric genomic library made in the YCpKan101 vector, provided by Jon Binkley and David Botstein (Stanford University, Stanford, CA) by complementation of the growth defect of ypk1G490R cells on YPUAD 2 mM EGTA at 37°C. The YPK1 gene was subcloned from this plasmid by ligating the 2.8-kb BstBI fragment into the ClaI site of YCp50, resulting in LHP1086. / q5 |8 _% k1 y' `" Z4 s7 X- F ; b2 _ c4 h; ~7 X! m! Y2 w1 rThe mutation in ypk1G490R was determined by gapped plasmid repair (Rothstein, 1991). The gapped YPK1 plasmid was purified and transformed into ypk1G490R and YPK1 cells. Repaired plasmids were purified from yeast cells and transformed into E. coli. Bacterial plasmid DNA was purified and sequenced. Two plasmids recovered from ypk1G490R cells were each found to have a single point mutation in codon 490.: a. q- r% [0 U9 Y# h t# c+ T
9 U" p* N- [- }; \. R) ^# `Internalization assays 8 _) W* m5 ?7 Z' F7 y+ ], b( @ ' I' j( z) a7 D, c* J5 I: iAll -factor internalization assays were performed essentially as described (Dulic et al., 1991) using the continuous presence protocol. Conditions for growth are indicated in the figure legends. Cells were harvested in early logarithmic phase and shifted to the assay temperature for 15 min before the addition of 35S--factor. Each data point represents the average of at least three experiments. The error bars represent the standard deviation. LY localization assays were performed as described (Dulic et al., 1991). Cells were grown in YPUAD at 24°C to early logarithmic phase and 2 x 107 cells were harvested by centrifugation. After 30 min or 1 h incubation with LY (Sigma-Aldrich) at the indicated temperature, cells were washed and resuspended in phosphate buffer and mounted on a slide with an equal volume of 1.6% low-melt agarose. Cells were viewed using an LSM410 confocal microscope (Zeiss) with FITC or with differential interference contrast (DIC) optics. All FITC images within a figure were taken using identical parameters. ' H2 }! |! r9 T; ^$ n- L B% S' d9 O+ T/ P( \2 Z
Cell lysates and immunoblots 9 S2 V) K) T1 E2 \8 e/ c$ D' X8 \: F7 q# F5 C( |1 Y
Lysates for HA-Ypk1 immunoblots were prepared after growth in YPUAD at 24°C (Horvath and Riezman, 1994). Lysates were resolved by SDS-PAGE on 10% gels and transferred to nitrocellulose. Membranes were first probed with -HA antibodies, then with HRP-conjugated goat -mouse antibodies (Sigma-Aldrich). The blots were developed using SuperSignal ECL reagents (Pierce Chemical Co.). Lysates for Ste2 immunoblots were prepared as described by Hicke and Riezman (1996), except cycloheximide treatment was not performed.1 W! c |5 `! O7 E& ~, J0 s9 m
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Footnotes) w) t4 Q7 ~4 K8 P G! D
+ m7 v* q- P! U$ H9 U4 sA.K.A. deHart and J.D. Schnell contributed equally to this work.# ~- x& q; t' r; D
7 l3 Z% X# e" y S6 _* Abbreviations used in this paper: DIC, differential interference contrast; HA, hemagglutinin; LY, Lucifer yellow. ! k- D. q/ f- r! P4 T. |9 [. Q5 m% I+ ?* ]
Acknowledgments; E9 u2 j0 K. q& c
! q8 y8 i' U- w& W4 eWe gratefully acknowledge the gifts of the YCpKan101-based genomic library from Jon Binkley and David Botstein and the pkhts and related pkh strains from Kunihiro Matsumoto. We thank Robert Lamb for generously providing HA antibodies and for the use of his confocal microscope. We thank Melissa Starkey for her assistance in this project. The manuscript was improved by the critical comments of Greg deHart and Marija Tesic. 3 @! C2 |2 v. }' L- j) x8 R m1 ] U+ ^7 W9 P$ L6 t# LThis research was supported by the Burroughs Wellcome Fund, the Searle Scholar program, and the National Institutes of Health (R01 DK 53257).Revised: 27 November 2001References & i5 b- w) l) Y5 ?1 N0 i4 B; C1 f g; l/ [- @6 n8 {
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我来看看！谢谢 作者: aakkaa 时间: 2015-5-28 09:54
我等你哟! 作者: nauticus 时间: 2015-5-29 21:08
很好！很强大！ 作者: 陈晴 时间: 2015-5-31 15:18
应该加分 作者: nauticus 时间: 2015-6-2 07:25
干细胞研究还要面向临床 作者: haha3245 时间: 2015-6-11 14:41
哈哈,有意思~顶顶 ，继续顶顶。继续顶哦 作者: tempo 时间: 2015-6-14 02:01