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Ribosome display and related technologies : methods and protocols % @7 J5 R$ S8 l% K3 z* ^7 v2 U
edited by Julie A. Douthwaite and Ronald H. Jackson. — New York : Humana Press, c2012. – (58.17/M592/v.805) 0 x p- f2 U$ X& A3 H ' {& {. `% M. s! x+ L h4 C7 \ Contents 4 `! {$ K2 e$ w4 N7 O! z 2 a' R9 L2 w6 ~1 p7 t/ f# C; L Preface3 I2 u1 ^! q: ^
Contributors ! d! k* M: k# T A* l PART I REVIEW3 P9 L `5 e( O, f2 v
1 Ribosome Display: A Perspective- D* H( _6 I, {+ a
PART II TRANSLATION EXTRACT PREPARATION1 Q& \0 K- A" q% ^5 G5 K
2 Preparation and Testing of E. coli S30 In Vitro Transcription Translation Extracts ! a$ B% w+ L0 ?1 P2 H0 s PART III BASIC RIBOSOME DISPLAY AND RELATED SELECTION METHODS ! p) f% @) V# D& g1 V, q, L 3 Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate 455 G& W. I5 M, k) e% e1 W
4 Stabilized Ribosome Display for In Vitro Selection # ^3 t& x7 u P2 D' Z% V# x 5 Eukaryotic Ribosome Display with In Situ DNA Recovery 75 O6 _1 l/ ~0 E: m+ {! _* w
6 mRNA Display Using Covalent Coupling of mRNA to Translated Proteins 87 1 X% O9 c9 U: M0 a. W 7 SNAP Display: In Vitro Protein Evolution in Microdroplets 101 & v% s: D" l$ G 8 cDNA Display: Rapid Stabilization of mRNA Display 1134 r# Y8 N: V8 L- j
PART IV APPLICATIONS OF RIBOSOME DISPLAY METHODS USING NATURAL AMINO ACIDS ; Y# P8 s, V2 L9 B/ S. O* F 9 Optimisation of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis8 _& w) ^( S% o5 \+ ]8 [
10 Affinity Maturation of Phage Display Antibody Populations Using Ribosome Display4 R* T5 A6 C+ P' T' n7 _# I
11 Evolution of Protein Stability Using Ribosome Display7 i) w3 a9 y4 P5 Z
12 Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries: k0 I) ]# I5 X/ E" E' \) E
13 Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display 2 Z! ^/ y! A" x- i8 k 14 Peptide Screening Using PURE Ribosome Display6 T$ ^$ t/ E% P* e' ]
15 Rapid Selection of High-Affinity Binders Using Ribosome Display 261: ~+ }3 z% S! q9 d
16 mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries 287 . Y! _0 y2 u2 j( W7 C9 h+ v 17 Identification of Candidate Vaccine Genes Using Ribosome Display 299 1 i4 X+ ~" ^+ ~ 18 Ribosome Display for the Selection of Sac7d Scaffolds 315 / ^. @/ H5 p, B PART V INCORPORATION OF NON-NATURAL AMINO ACIDS FOR SELECTION BY RIBOSOME DISPLAY AND RELATED METHODS' t' H* \! _, K+ ^+ f
19 Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers 335 * L$ P; J6 x. V) n. o% o7 { 20 Update on Pure Translation Display with Unnatural Amino Acid Incorporation 349 u W; |) Y- I* _. J 21 In Vitro Selection of Unnatural Cyclic Peptide Libraries via mRNA Display 367. S1 s4 a4 e7 A6 B0 f% @. m7 t
PART VI CASE STUDIES* x; O4 n8 l/ g; O n/ [5 _
22 Optimization of CAT-354， a Therapeutic Antibody Directed Against Interleukin-13， Using Ribosome Display & r e2 N- {) p7 |* {$ F 23 Affinity Maturation and Functional Dissection of a Humanised Anti-RAGE Monoclonal Antibody by Ribosome Display 403 C! i4 T3 g5 ?. Y8 W Index 423 5 Y5 M' A9 @7 T Z6 h& M& J+ v9 {7 T 作者: goast8 时间: 2013-1-14 10:13