' C$ P6 N* Y- v {0 X: m6 UThis paper reports thenovel creation of human embryonic stem cells from somatic nuclei. It hasreceived massive media coverage and is surely pencilled in as a strongcandidate for scientific publication of the year. 2 X- h! C' _& d, j4 e
! T3 _9 f) n5 t/ m1 G8 TIt does however haveseveral examples of image reuse which might be of interest to PubPeer membersand readers. 9 p, U& c1 M5 |) D3 m% e1 E 1 A* L1 m1 Y8 ` 0 D7 v1 q& L4 z/ Z2 F* K5 _9 z h7 C# D6 W# z: E
Fig. 2F NT-ESC colonywith typical morphology derived from a caffeine-treated SCNT human blastocyst.8 D5 I8 i4 C! G! R
; r7 y5 \; {: P7 C- F 9 H/ v' C/ a |) _1 C" V G: N0 Y1 G' n6 P0 l( a/ u# NFig. 6D pluripotencymarkers (top left) 7 [* ^( A0 H& ]7 O
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Fig. 6D pluripotencymarkers (top right)& o+ s1 u& t' N# V
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Fig. s5, Expression ofPluripotency Markers in Human NT-ESCs and in Control IVF-ESCs Detected byImmunocytochemistry(top right) _# E1 k8 f$ p- E
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9 W" V1 I: w% s3 }Figure S6 MicroarrayScatterplot Analysis of Biological Replications within Each Cell Type(topcentre and top right are the same image) & Z& Q x" k; [' ~: P0 s5 e x( t0 Z% Y7 `, f7 c) Q
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( J& K4 D% g) T/ q6 n! _2 {2 o- Fig. 2F is a slightlycropped version of the cell microscopy image in Fig. 6D top left. & p2 f+ C' w2 m( ?" U
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- Fig. 6D top right, thecell microscopy image is a slightly cropped version of supplementary Fig. s5,top right. The cells in 6D are labelled as "h-ESO-NT1 Ph" yet infigure s5 they are labelled to be "hESO-7". We understand the formerto inherit caffeine-treated somatic nuclei whereas the latter are original stemcells. 8 e$ c, }0 X/ x) J; Q3 V. K, L+ X0 R
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Under pressure toassemble the figures for rapid publication, one can understand making a cut andpaste figure assembly mistake. Nevertheless it should be noted that imagecropping does take extra work. ! `* a/ y% M. b
( H1 u+ ~7 K5 X7 J4 n- Figure S6 top centreand top right are the same image. 2 s: e1 J7 l9 ]( }- S6 e: b* T, V
- Figure S6 middle leftand lower right are reported to be biological replicates of microarrayexpression quantitation. In those cases however the narrow spread indicatesthat the data are extremely similar and are only understandable as technicalreplicates (where the same RNA sample is hybridised to two different arrays).It is useful to do technical replicates to control experimentalreproducibility, but biological replicates are more valuable when reportingresults. They are not the same thing and should not be conflated. (For therecord, we did check the microarray data deposited at Gene Expression Omnibus(GSE46397)).+ C" A6 X; E" s3 M
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Lastly we note that, inthe paper, it is recorded that the journal Cell accepted this paper just 4 daysafter submission. Perhaps, under the circumstances, the pre-publication peerreview had to be a little hasty? At least here at Pubpeer, while conductingpost publication review, we can take as long as necessary to make up for thatlost time. & O2 U( D3 {2 S2 Y! d
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3 ^0 ~/ I; t! t韩国科学家黄禹锡Science上的两篇人胚胎干细胞造假论文(Science 2004, 303: 1669和Science 2005, 308: 1777)0 r+ V7 W( L' O. U( z
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1. Science 2004, 303: 1669 ( d* L: s$ t1 m+ Z9 f
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This article has been retracted( {) S7 p& Z9 g* U2 f9 Y6 f
: r5 ?* K% V4 j2 o" B0 S# L/ w' P+ BPublished Online February12 2004, Science 12 March 2004: Vol.303 no. 5664 pp. 1669-1674 DOI: 10.1126/science.1094515* X! m2 e: f& }! R! z. w$ m
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REPORT3 [( w5 C* ^9 j# j' U# E2 G) d
. D, W. d) Q4 ? t1 X9 t/ o. f, TEvidence of a PluripotentHuman Embryonic Stem Cell Line Derived from a Cloned Blastocyst & u. {& [2 f& i( C 2 W& }9 ? y. P9 c* {http://www.sciencemag.org/content/303/5664/1669.full& Y% o$ Q) L" z9 d1 b
0 Y/ @; t8 P# f3 L3 j) x 9 T) f+ [) K8 a1 @% @8 d# W ( K* u ?& i' k$ BFig. Expression ofcharacteristic cell surface markers inhuman SCNT ES cells. SCNT-hES-1 cells expressedcell surface markers9 [& Y& v# M% ^& E5 Q! ^
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2. Science 2005, 308: 1777 {3 ]" \- b6 n' J, i9 n) X+ L
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This article has been retracted 5 L/ m" ?' k3 u5 W/ h- d1 u% [9 L 8 N) g+ f% P' K; j+ m# oPublished Online May 192005, Science 17 June 2005: Vol. 308no. 5729 pp. 1777-1783 ' z2 |+ a) c7 Q ; A% ^' F1 k/ h M) vDOI:10.1126/science.1112286: n! w& ^: D6 m3 H# m
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REPORT9 I' m: T' i; W E
( ~" N) j9 M5 S( T4 sPatient-SpecificEmbryonic Stem Cells Derived from Human SCNT Blastocysts8 S+ R2 [* }) a! }# O5 D
V+ Z7 i* ~% ~2 M4 q http://www.sciencemag.org/content/308/5729/1777.full1 T1 C' S. G5 h" n$ l' v0 |
a! H% o- P; Z2 n3 y/ f 5 d$ e [% B4 T( f* D$ K2 @& I % A3 `# F/ O2 |1 wFig. hESC linesestablished from NT blastocysts using patient somatic cells (NT-hESCs) arepluripotent with normal karyotypes7 y8 t9 j& B+ }& m$ s: }
7 P: o4 @" n' ^9 i6 `2 R- M8 U3. Cell Stem Cell, Volume1, Issue 3, 13 September 2007-cover paper 和Science,Volume 315, Issue 5811, 26 January 2007-cover paper6 U& H3 r: c' m+ h; S, C! O" Y% t7 e
% |" K3 [) @4 k2007年11月是一个令人感慨的岁月，黄禹锡被废掉500天之后，达利教授功成名就！哈佛大学兼Boston儿童医院Daley实验室乔治·达利(George Daley)教授从黄禹锡Sciene“造假”论文中获取灵感，当年发表在Cell Stem Cell上的一篇封面论文宣布：2004年，由韩国胚胎干细胞专家黄禹锡博士建立的人类疾病基因胚胎干细胞株，已被该研究团队确认，这些细胞株的建立方法是不含外源性基因污染的单性繁殖胚胎干细胞，很有可能是一项历史性的创举。同年他们还利用小鼠的未受精卵，产生所谓的孤雌生殖（parthenogenetic）胚胎干细胞，证明孤雌胚胎干细胞的组织相容性，并发表了Science封面论文。 0 G7 E4 v& Q9 A& {# C ' r, Z! d. S. X: l; l6 a& J1. 区分孤雌和体细胞克隆胚胎干细胞的方法 5 z/ }% X w/ q1 `/ P, G$ {. S: p7 ?) D! d; F5 ^, X( s
Cell Stem Cell, Volume 1,Issue 3, 346-352, 13 September 2007, doi:10.1016/j.stem.2007.07.0016 J# R8 k: E( T% B
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