/ j1 i: V+ K5 }& L; | 该新导管已经在欧洲进行临床试验。1 ?" ~) i5 C4 V9 d* V
$ D j& ]0 k0 _1 _" C$ Q
Optimized Delivery System Achieves Enhanced Endomyocardial Stem Cell Retention. k9 a9 v- |) J( o
. L! |2 ~7 C$ A+ W- d4 i" v6 ]) _ Atta Behfar, Jean-Pierre Latere, Jozef Bartunek, Christian Homsy, Dorothee Daro, Ruben J. Crespo-Diaz, Paul G. Stalboerger, Valerie Steenwinckel, Aymeric Seron, Margaret M. Redfield, and Andre Terzic" k/ a' c5 n% Z
; z9 J8 u5 {1 {2 M0 y5 {! T8 P Background—Regenerative cell-based therapies are associated with limited myocardial retention of delivered stem cells. The objective of this study is to develop an endocardial delivery system for enhanced cell retention.# X- F% U) h! g( j7 h! N% A
8 T2 N+ d9 L+ G& z Methods and Results—Stem cell retention was simulated in silico using 1- and 3-dimensional models of tissue distortion and compliance associated with delivery. Needle designs, predicted to be optimal, were accordingly engineered using nitinol, a nickel and titanium alloy displaying shape memory and superelasticity. Biocompatibility was tested with human mesenchymal stem cells. Experimental validation was performed with species-matched cells directly delivered into Langendorff-perfused porcine hearts or administered percutaneously into the endocardium of infarcted pigs. Cell retention was quantified by flow cytometry and real-time quantitative polymerase chain reaction methodology. Models, computing optimal distribution of distortion calibrated to favor tissue compliance, predicted that a 75°-curved needle featuring small-to-large graded side holes would ensure the highest cell retention profile. In isolated hearts, the nitinol curved needle catheter (C-Cath) design ensured 3-fold superior stem cell retention compared with a standard needle. In the setting of chronic infarction, percutaneous delivery of stem cells with C-Cath yielded a 37.7±7.1% versus 10.0±2.8% retention achieved with a traditional needle without effect on biocompatibility or safety. ( X$ Z. c, p( ~, [) X; ^ y6 S5 G ^3 X7 B1 \9 P Conclusions—Modeling-guided development of a nitinol-based curved needle delivery system with incremental side holes achieved enhanced myocardial stem cell retention.作者: 239075369 时间: 2014-1-10 23:22