加拿大研究机构Kevin Gregory-Evans小组成功将hESC定向诱导为感光前体细胞，仅用17天的时间，诱导效率达到78%。这对感光细胞来源是一个极大的新闻，解决了感光前体细胞作为种子细胞的来源问题。为向临床转化更近了一步。 . |1 R8 A0 Q; f8 Y2 C6 }, w% Z3 V! Y3 ]* a* z& Y
还不知道怎么粘贴图片和发表链接，下面是文献题目。Yanai A, Laver CR, Joe AW, Viringipurampeer IA, Wang X, Gregory-Evans CY, et al. Differentiation of Human Embryonic Stem Cells Using Size-Controlled Embryoid Bodies and Negative Cell Selection in the Production of Photoreceptor Precursor Cells[J]. Tissue engineering Part C, Methods 2013.作者: 细胞海洋 时间: 2014-1-21 09:21
Title " v1 [3 V) u5 O# uDifferentiation of Human Embryonic Stem Cells Using Size-Controlled Embryoid Bodies and Negative Cell Selection in the Production of Photoreceptor Precursor Cells % ?7 c; C4 e) v1 K8 e0 ^# z& c; H5 w; X8 `& A7 o' x% B- q0 c
Abstract1 t- t, I. O, Q' N! {' K a, X% |
We proposed to optimize the retinal differentiation protocols for human embryonic stem cells (hESCs) by improving cell handling. To improve efficiency, we first focused on the production of just one retinal precursor cell type (photoreceptor precursor cells [PPCs]) rather than the production of a range of retinal cells. Combining information from a number of previous studies, in particular the use of a feeder-free culture medium and taurine plus triiodothyronine supplements, we then assessed the values of using size-controlled embryoid bodies (EBs) and negative cell selection (to remove residual embryonic antigen-4-positive hESCs). Using size-controlled 1000 cell EBs, significant improvements were made, in that 78% CRX+ve PPCs could be produced in just 17 days. This could be increased to 93% PPCs through the added step of negative cell selection. Improved efficiency of PPC production will help in efforts to undertake shorter and larger preclinical studies as a prelude to future clinical trials. 2 l, R, i3 C$ a " j3 i: C+ s; ?3 PYanai, Anat $ E7 k- I. M( J$ v1 r' YLaver, Christopher R J 5 c: K& x6 P1 u9 X) [: |8 _Joe, Aaron W7 c. J. j! c, W( N5 \4 j, o
Viringipurampeer, Ishaq A, k0 K6 i8 }: |: f; \
Wang, Xia" x% O6 S( P& C8 I5 }! y
Gregory-Evans, Cheryl Y2 }/ o! K( K1 i8 \+ [+ Y$ E1 V
Gregory-Evans, Kevin 9 v# U" W; s$ g- P WENG 1 [: `5 P5 i# V3 p2013/02/01 06:006 R2 ?5 c) z& x; i
Tissue Eng Part C Methods. 2013 Mar 15. " \/ S6 l( f+ Z; s6 S+ } ~8 w+ v5 W. a" ~. j$ q8 _" T/ l3 s
URL3 y2 O2 X) N: F/ T) a9 [
http://www.ncbi.nlm.nih.gov/pubmed/233633704 F% o! W" J" S1 V) T# w4 \6 U, p9 \
http://online.liebertpub.com/doi/abs/10.1089/ten.tec.2012.0524作者: shaozi402 时间: 2014-1-21 13:32
另外，又附加一篇hESC高效诱导为感光前体细胞的详细protocol 1 _. M) @: ^0 k5 R$ ITitle9 n$ a _8 @' F9 s. t9 e
Efficient Production of Photoreceptor Precursor Cells from Human Embryonic Stem Cells C I$ \1 G/ d( q8 z+ t0 O+ L % S2 Y9 S7 R5 R, gAbstract H! x1 A; l. c- R2 ^6 p' o; {
Transplantation of photoreceptor precursor cells (PPCs) differentiated from human embryonic stem cells (hESCs) is a promising approach to treat common blinding diseases such as age-related macular degeneration and retinitis pigmentosa. However, existing PPC generation methods are inefficient. To enhance differentiation protocols for rapid and high-yield production of PPCs, we focused on optimizing the handling of the cells by including feeder-independent growth of hESCs, using size-controlled embryoid bodies (EBs), and addition of triiodothyronine (T3) and taurine to the differentiation medium, with subsequent removal of undifferentiated cells via negative cell-selection. Our novel protocol produces higher yields of PPCs than previously reported while reducing the time required for differentiation, which will help understand retinal diseases and facilitate large-scale preclinical trials.$ v5 r, q. e+ o I' m: k
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Yanai, Anat( S7 E0 V$ d& M2 C c, o
Laver, Christopher' G7 [, Y- E+ a. u4 A. @6 f
Joe, Aaron W/ y$ l6 Q- @6 r% O& E
Gregory-Evans, Kevin ( G# L) g8 \) w/ ]! `8 a9 r* xENG 8 z4 B9 v( y e: iClifton, N.J. 1 M3 `5 a/ Z$ y2013/12/05 06:00 0 {8 v( Z+ E( X! |3 A9 ~. zMethods Mol Biol. 2013 Dec 4.' k* \/ i# E7 Y& S" x