本帖最后由 细胞海洋 于 2014-5-23 07:33 编辑 4 R8 T( ?. m; Y4 }) g' _' ? . [0 g% Z! L* n, o, P 首先很抱歉现在才继续第六期的网络会议。从这学期伊始Damon就发电子邮件甚至打电话邀请相关人士来做报告，但是大多被婉拒了。现在还在准备GRE，今年10月份就开始申请了，亚历山大- -！$ i6 n2 |+ n' d; ? m7 O3 E
接着感谢主讲人李侠，也是我们辛勤的版主，能在百忙之中给我们讲解这一课题。会议时间暂定为2014年6月7日晚7：30。欢迎广大会员积极参与研讨！参与研讨和在本贴下跟帖支持或提建议的会员都会获得不限量的威望和包包奖励 - e1 p; G" W5 |7 s" f9 ~3 s N+ P) L 本次网络会议依然依靠YY网络语音平台，请大家加YY群（ID：8298994），附言干细胞之家，将群名片改成论坛昵称。届时我将大家拉入平台。后期资料（PDF课件和屏幕录像）我分享到 百度云。 为了保证会议质量，请大家将电脑YY客户端更新至最新版本。 9 u- `. l4 o. Y7 w: S/ l2 @8 p0 Z; A+ J, b8 ~8 v- m
安全iPSCs的获取方法：重组蛋白诱导iPSCs
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诱导多能干细胞(induced pluripotent stem cells, iPSCs)是一类最初通过特定因子(Oct4, Sox2, Klf4和c-Myc)在分化的体细胞中异位表达，使成熟的体细胞重编程为具有胚胎干细胞(embryonic stem cells, ESCs)样生物学特性的细胞。iPSCs为药物筛选、疾病诊疗、动物遗传资源保护以及转基因动物创制等研究提供了一种崭新的材料，在医学、生物学基础研究和畜牧业上具有极其重要的学术价值和广阔的应用前景。* l6 H* [1 J1 x7 [& p
众所周知，重编程的低效率和安全性等问题，尤其是iPSCs的安全性严重制约了iPSCs的广泛应用。目前，相对较安全的方法有蛋白诱导iPSCs和小分子化合物诱导。其中小分子化合物仅目前仅邓宏魁一家，而蛋白介导的iPSCs虽说依然存在各种问题，但重复性较好（据我所了解，目前有九个研究组报道了相关的案例）。+ E# \2 f' B1 t; T
猪作为重要的经济动物，在我国畜牧业中有举足轻重的作用，而且猪的遗传、代谢、生理生化及基因序列等方面均比小鼠更接近于人类，因而正逐渐成为人类异种移植和再生医学研究的理想生物学模型。然而，目前尚未见到用蛋白诱导猪iPSCs的相关报道。因此，本次会议将首先阐述iPSCs的历史和现状。其次，重点分析重组蛋白介导iPSCs技术中的关键步骤（蛋白制备，蛋白入核及定位，重编程策略和存在的问题）。最后，对猪iPSCs的研究进展和重组蛋白诱导猪iPSCs的策略做一个简单的总结和规划。 : U- q2 D! n- ?. P+ \+ X1 |0 O+ l[attach]61561[/attach]9 G5 d2 ]; U& K6 j
4 i6 |3 Y$ Y3 Z* v/ w) O0 R主讲人简介：李侠，硕士 ' g( ?8 `7 d v4 }- V8 N, L单位：安徽农业大学，动物科技学院，安徽地方畜禽遗传资源保护与生物育种省级实验室 : z1 I0 l" h- `! x8 `# O7 l* I1 n* n ~) D" G3 t; X
主要研究领域：猪，诱导多能干细胞，重组蛋白，慢病毒, w2 N9 O/ x( k% y( f
成绩：已发表SCI论文2篇（1篇见刊-非一作，1篇accept）；中文2篇（1篇见刊，1篇接受）；1篇SCI和1篇中文在投& g7 S- u7 c6 L6 @ J L* {! m
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主要受教育经历：$ c* @, @1 S! r
2008/09-2012/06，安徽农业大学，动物科技学院，本科 5 I0 H7 k" j* f2 r2 j1 }9 k2012/06-至今，安徽农业大学，动物科技学院，硕士' J3 F k; ^ v
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2. 2009-Generation of Induced Pluripotent Stem Cells Using Recombinant Proteins' O) I/ b& O0 m; Y2 m
3. 2012-Activation of Innate Immunity Is Required for Efficient Nuclear Reprogramming ! p, E" \) @$ @ x4. 2012-Reprogramming of somatic cells via TAT-mediated protein transduction of recombinant factors% d/ }9 h6 E- n# ]
5. 2014-Partial Somatic to Stem Cell Transformations Induced By Cell-Permeable Reprogramming Factors 9 u6 g# X' c& Y f6. 2008-Protein production and purification # h" I2 m9 h% Y7 p# v7 s7. 主讲人最新综述：Generation of pluripotent stem cells via protein transduction1 u: R* I4 e% z7 i A% o
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[attach]61562[/attach]$ R0 g+ w6 S0 ?* i( ]
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主讲人最新综述：Generation of pluripotent stem cells via protein transduction下载：$ Q( Y& v3 n* |" v
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3 x4 V+ [4 ~: q9 N9 R 作者: 孤野知—少帅 时间: 2014-5-21 22:23
Great organization! Thanks for your efforts.作者: Damon-Salvatore 时间: 2014-5-27 14:47
回复 ccwei0615 的帖子 . s7 b/ |. `( d8 m3 {) e9 K : N Q) X- e7 a4 CMy pleasure. Haven't heard from you for quite a while. I noticed an uncritical typo in a journal and email to inform the magazine today. What do you think of it? should I really do that? or am I too earnest about it?作者: ccwei0615 时间: 2014-5-27 16:00
本帖最后由 ccwei0615 于 2014-5-27 16:07 编辑 1 H# C5 }* z. u ! |" ~5 b% F' y, a3 b0 F: PYes, I am busy on looking for founders to start our hypoxia MSC project. Hope this thing could be done soon. Our US patent is almost be issued and the trial also will be reviewed in next month. So glad to know you finish the article. Never mind for your concern. After you submit your article, the editor usually will give you a chance to double confirm your final version. You could make correction or even update any new information on it. Do you have any idea when will it be published? Finally, I would like to know in your opinion whether the 3D kidney really be created and with biological function? I really doubt on it.作者: 芦苇浅 时间: 2014-5-27 19:24
回复 ccwei0615 的帖子 % e! {; O$ o# x& `5 h& m& n+ L0 ]( |- p3 F% N; t+ f! V5 R
Maybe I didn't make myself well understood. The typo I found is in a published journal of someone else instead of mine. Whatever, I just emailed. Hope you can find some extravagant founders to support your project soon. Indeed, I don't think very high of 3D printing kidney personally, even the prototype was printed successfully in 2011 by Atala. What is attracting my attention in organ regeneration now is blastocyst complementation, about which I made a video # w& e) R3 S! N- _: N* R(http://v.youku.com/v_show/id_XNzA1NzgxNTI4.html).作者: manybit 时间: 2014-5-28 10:22
支持！
顶起顶起！！！
