/ W+ m) |) E6 M# P5 ][attach]62633[/attach] + ?* O, j v" P4 n! o: ^0 R1 I4 @" T- `+ X
此外您也可以参考下我们自己的茜素红染色操作步骤。谢谢！ * r# `( ^4 p7 p' L e' s 9 a( o* u. F1 Q2 Q- S( m2 l( ^) cAlizarin Red S Staining Protocol:* ?3 h: p; M' k4 v; R; F * Y5 e! ?: ~, ]1 R' k' ]6 y& y( Q
Alizarin Red S (Sigma A5533-25g)9 t0 j. p; s1 c- r: F! V9 E
FW = 342.3g/mol' Y' g, H' S" B9 I) q$ x: k
2 Q# @( N! u% K* nPrepare 40mM Alizarin Red S Staining Solution (100mL): ( |0 d) F& f% U) ?3 t5 \4 { ) [ V& B- I, W6 b1 `; Q8 y
1. Dissolve 1.3692 g Alizarin Red S in 80mL of dH2O; V3 Z: y/ r( K8 }
2. pH Alizarin Red S solution to 4.1 with 10% (v/v) ammonium hydroxide+ x M1 Q2 d5 V& D# X
3. Top up volume to 100mL using dH2O 2 ]: _3 u% H. X& T5 S; }: Z7 S 3 D9 O0 m3 R. a+ X' L; LStaining Protocol: (6 well plate)3 O( f- S6 }- }: m
0 _$ c v7 r( Q: e" k" r% z9 L5 \- A" q1. Wash each well with 1xPBS " d7 x- c) w9 T7 P2. Fix cells using 10% formaldehyde at room temperature for 10min ! V" i: j. Z5 l& L3. Wash cells two times with dH2O8 e5 ?/ t& a2 \* m8 d$ ]7 N+ R J
4. Add 1mL of 40mM Alizarin Red S Staining Solution into each well and stain at room temperature for 20mins on a shaker ! m% ^2 Z3 G, D5. Aspirate off excess stain and wash four times with 4ml of dH2O for 5mins each time on a shaker作者: liluoshushu 时间: 2014-8-20 12:54