! x2 T0 m. I, f2 ~2 Y& N6 j: c2 s此外您也可以参考下我们自己的茜素红染色操作步骤。谢谢！ 8 D4 j7 ^5 X0 G) x( c6 M( W5 F $ y- G, C, Y/ e' j+ E% {. XAlizarin Red S Staining Protocol:' e: r9 i* Y: o! y' M" a" ^ A* p8 a3 }) s
Alizarin Red S (Sigma A5533-25g) ' i+ p: B- M8 M) wFW = 342.3g/mol2 Q @% w% C2 w( S5 K# M" f
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Prepare 40mM Alizarin Red S Staining Solution (100mL):/ z( ?4 K0 @& ~. P
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1. Dissolve 1.3692 g Alizarin Red S in 80mL of dH2O ; F' d- C& m6 m! w# w8 }9 t2. pH Alizarin Red S solution to 4.1 with 10% (v/v) ammonium hydroxide# {7 e5 x+ B9 b# P2 N% R: B4 _
3. Top up volume to 100mL using dH2O1 o+ C, i0 T9 Y
! O2 f; ^. d# U- i( }0 t" J. ], }Staining Protocol: (6 well plate)& I8 i) j9 V& I. Y. {6 _: c9 g
- n# P- z% M0 L1 _% J; J2 V u' o* D1. Wash each well with 1xPBS& Q: @& C4 D8 n& C( m Z; u' F
2. Fix cells using 10% formaldehyde at room temperature for 10min3 r% }5 t3 W' ` n6 o0 H3 u1 P
3. Wash cells two times with dH2O + I, \" w o, f. t4. Add 1mL of 40mM Alizarin Red S Staining Solution into each well and stain at room temperature for 20mins on a shaker6 c5 x; [. d ^$ Y: O6 S4 b1 H' f
5. Aspirate off excess stain and wash four times with 4ml of dH2O for 5mins each time on a shaker作者: liluoshushu 时间: 2014-8-20 12:54
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谢谢作者: kelvinlin 时间: 2018-1-29 11:29