标题: PDF电子书：Cancer Cell Culture Methods and Protocols（SecondEdition） [打印本页] 作者: fenlichong 时间: 2015-8-7 19:42 标题: PDF电子书：Cancer Cell Culture Methods and Protocols（SecondEdition）
本帖最后由 细胞海洋 于 2015-9-17 12:45 编辑 + A( e- G8 h+ u5 @7 U. { w! V4 R7 l$ d8 [* w7 W
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Contents $ ^' C% ?$ f9 E" G- S* y9 L: ?Preface............................................................. v 9 _3 I( x2 _. lContributors......................................................... xi # \% i0 I7 f& W1 K% o- Z 1 Cancer Biology ................................................... 1 6 s' C4 U) K0 L; _7 Y( VIan A. Cree ; G) W5 ]9 @$ u+ g! \) D 2 Principles of Cancer Cell Culture ..................................... 13 # f" m2 A: V- b3 _$ S) U9 R" q) _Ian A. Cree 8 ], U6 ~# S9 `1 t: c 3 Storage of Cell Lines............................................... 27 ' h* R( e$ y) P, gKatharine A. Parker ' ~* F, t3 N/ d- F! _8 @( c 4 Characterization and Authentication of Cancer Cell Lines: An Overview ........ 35' i5 s8 E, l' w+ b2 d
Yvonne A. Reid 1 O# T8 y9 [- d G. r8 ?3 Q- } 5 Online Verification of Human Cell Line Identity by STR DNA Typing ......... 45+ u4 A \' b0 c4 c
Wilhelm G. Dirks and Hans G. Drexler 7 Z* M8 D K, F: @' g" B- M
6 Cytogenetic Analysis of Cancer Cell Lines............................... 57 1 k s6 H4 i- P' T4 w8 |# QRoderick A.F. MacLeod, Maren Kaufmann, and Hans G. Drexler : S& u R& K. ~* T7 N
7 Cell Culture Contamination ......................................... 79 6 L# A3 `# \4 oGlyn N. Stacey ( I! }/ q& f3 S% j n$ F5 G# r& R. E3 f p
8 Detecting Mycoplasma Contamination in Cell Cultures # { E% a( x% nby Polymerase Chain Reaction ....................................... 933 \$ g- T. P* M$ n( B
Cord C. Uphoff and Hans G. Drexler 8 B% B* p S$ J p( }) c0 b# I" U
9 Elimination of Mycoplasmas from Infected Cell Lines Using Antibiotics ........ 105( u, B" y. Y4 v' P ]
Cord C. Uphoff and Hans G. Drexler : R" u+ n8 e/ g$ H, ^5 u" c
10 Quality Assurance and Good Laboratory Practice ......................... 115 " y. N) G+ l/ p& d$ j& r: D+ mLouise A. Knight and Ian A. Cree " U2 p: [0 ^0 }11 Generation of Lung Cancer Cell Line Variants by Drug Selection or Cloning .... 125# a* n, Y% n# ~+ L9 K5 c, u
Laura Breen, Joanne Keenan, and Martin Clynes ! m8 s: }6 s2 h+ G- r+ P12 Isolation and Culture of Colon Cancer Cells and Cell Lines ................. 135 4 x8 f) D8 p7 }- q7 z- LSharon Glaysher and Ian A. Cree + n9 K( H# d! }: Z% N
13 Isolation and Culture of Melanoma and Naevus Cells and Cell Lines........... 141 " {4 {# o- S( H# V4 V, MJulia K. Soo, Alastair D. MacKenzie Ross, and Dorothy C. Bennett ~( [) [' J! Z; t2 P* C0 N14 Isolation and Culture of Squamous Cell Carcinoma Lines ................... 1515 `8 W* y6 T: k9 `% w
Karin J. Purdie, Celine Pourreyron, and Andrew P. South ) @0 J1 ~! n( W$ \
15 Isolation and Culture of Ovarian Cancer Cells and Cell Lines ................ 161 4 l; Q x+ p. _- |, R7 @5 \Christian M. Kurbacher, Cornelia Korn, Susanne Dexel, Ulrike Schween, 9 O/ R3 e0 N# }0 ^# a" D. aJutta A. Kurbacher, Ralf Reichelt, and Petra N. Arenz , {+ t0 J% C: i! i/ i1 h Y, ?
16 Establishment and Culture of Leukemia–Lymphoma Cell Lines .............. 181 0 N# S5 `7 j' R5 v: l0 g* eHans G. Drexler / r! m- H# e# c6 k) Y' T4 C- t
17 Isolation of Inflammatory Cells from Human Tumours..................... 201 3 ^6 P3 g# Y9 l _Marta E. Polak $ E) O) y+ c4 f* S18 Isolation of Endothelial Cells from Human Tumors ....................... 209 6 {: a, h3 d1 Y& _! C) W- m c3 b, UElisabeth Naschberger, Vera S. Schellerer, Tilman T. Rau, + H5 [, q3 ^' c- r0 E% g" IRoland S. Croner, and Michael Stürzl 4 Q& i; L; T) }& o( e
19 Cellular Chemosensitivity Assays: An Overview ........................... 2196 |. A* I2 L- X. |- Z1 H7 k) W9 f
Venil N. Sumantran . J" N- I6 `" Z, v n8 R
20 Cell Sensitivity Assays: The MTT Assay ................................. 237 3 t: c8 A& j7 j+ A% G9 YJohan van Meerloo, Gertjan J.L. Kaspers, and Jacqueline Cloos 7 D% b: f, s. k5 D0 u
21 Cell Sensitivity Assays: The ATP-based Tumor Chemosensitivity Assay ......... 2470 Z( d" i* ~+ `5 N( e" K
Sharon Glaysher and Ian A. Cree % d. n. k' M' S$ W9 ?( \, X0 [! @* @- l
22 Differential Staining Cytotoxicity Assay: A Review ........................ 259$ ~1 I8 P' `& c. f; i" O, r& h& J6 x7 I
Larry M. Weisenthal ( j7 ]% R( n5 L5 t
23 Real-Time Cytotoxicity Assays ....................................... 285 " s% Q: O7 ^" Q/ @* d) Y& z! n# rDonald Wlodkowic, Shannon Faley, Zbigniew Darzynkiewicz, * S* L# c: i% [0 N5 `
and Jonathan M. Cooper 5 f8 c, L9 q1 Y( A24 Purification of Annexin V and Its Use in the Detection of Apoptotic Cells ...... 293 & C) `6 j8 q3 Q5 w/ E+ pKaty M. Coxon, James Duggan, M. Francesca Cordeiro, and Stephen E. Moss ! ^( C, V8 ?0 v' g# n25 Measurement of DNA Damage in Individual Cells Using the Single Cell Gel ; b. w% G3 q) {4 l8 r5 Q: k
Electrophoresis (Comet) Assay ....................................... 309 " i" ~0 p6 X$ m: ]% ZJanet M. Hartley, Victoria J. Spanswick, and John A. Hartley 4 O' V/ s: {; {* h5 L* _0 x, D
26 Molecular Breakpoint Analysis of Chromosome Translocations , b, [1 `! s% f7 ?$ x6 Q- u, F5 f+ z4 @in Cancer Cell Lines by Long Distance Inverse-PCR ....................... 321 7 R; N3 G7 {! x8 g1 JBjörn Schneider, Hans G. Drexler, and Roderick A.F. MacLeod 4 }. S+ ]5 Y9 f
27 Cell Migration and Invasion Assays .................................... 333 1 {+ r8 D+ b9 G0 j# c! ~2 T% H: zKarwan A. Moutasim, Maria L. Nystrom, and Gareth J. Thomas ( E. b( A) i$ ~4 h0 N5 h3 {! z
28 Angiogenesis Assays ............................................... 3458 W/ i, r, L$ [0 Z- y
V. Poulaki 8 Q6 Q# |, b) n
29 Flow Cytometric DNA Analysis of Human Cancers and Cell Lines ............ 3596 |+ }% f# E. b; p% `
Sarah A. Krueger and George D. Wilson 8 A# b2 x$ J% A4 q# s$ v
30 Expression Analysis of Homeobox Genes in Leukemia/ , b7 Q- Z+ J% _, P3 ]
Lymphoma Cell Lines ............................................. 3718 `# } R _' U! i
Stefan Nagel and Hans G. Drexler 7 t, u$ h {4 d$ j31 Measuring Gene Expression from Cell Cultures by Quantitative * T2 q8 Q- d: f- e3 X( `) \ RReverse-Transcriptase Polymerase Chain Reaction......................... 381( e! ?& \% Y u: p, z; V4 A( M1 a
Sharon Glaysher, Francis G. Gabriel, and Ian A. Cree ( z. R* v, `! s) w, j+ |32 Proteomic Evaluation of Cancer Cells: Identification of Cell Surface Proteins .... 395 4 D- P5 J+ h2 o% g& \7 c. w+ d4 HSamantha Larkin and Claire Aukim-Hastie 1 h3 r+ R; a: I# I33 Development of Rituximab-Resistant B-NHL Clones: An In Vitro 5 c9 Z3 A7 h7 g# W) Q
Model for Studying Tumor Resistance to Monoclonal Antibody-Mediated ( p* t6 [1 {8 k/ }$ t: e. ~Immunotherapy .................................................. 407 ' _$ u$ F! ?% M6 k# rAli R. Jazirehi and Benjamin Bonavida * x. h! w. a6 M. t# C' ?
34 Analysis of Drug Interactions ........................................ 4215 ~/ u$ ?9 p+ A4 k
Irene V. Bijnsdorp, Elisa Giovannetti, and Godefridus J. Peters u5 u! V) E3 h5 ^% T; Y3 @( g( s35 Transfection and DNA-Mediated Gene Transfer .......................... 435) J$ Q& b9 M' J' E
Davide Zecchin and Federica Di Nicolantonio % Q+ `' `. _9 O' v, d/ @. `' \
ixContents - ~0 `. B* \' t' s; M5 C36 Drug Design and Testing: Profiling of Antiproliferative / W$ j" h! @* W1 L, I/ W/ I; K
Agents for Cancer Therapy Using a Cell-Based Methyl-[3H]- : ?+ O* y! A) Y, F) i
Thymidine Incorporation Assay ...................................... 451 ' x* O8 O! b+ U$ a% ~Matthew Griffiths and Hardy Sundaram ; ?1 j1 Q1 {! O( n5 o37 Feeder Layers: Co-culture with Nonneoplastic Cells ....................... 467( T- I( [) o5 \) o
Celine Pourreyron, Karin J. Purdie, Stephen A. Watt, and Andrew P. South 6 H! @+ z) l. J
38 Xenotransplantation of Breast Cancers ................................. 4719 |( w1 {4 ^! @/ s9 E
Massimiliano Cariati, Rebecca Marlow, and Gabriela Dontu & }1 [4 o1 p6 m0 y8 j6 WAppendix A ......................................................... 483 : m% N) s( D2 ]' @ RAppendix B ......................................................... 487 / Y! K! `% ?# L+ e& |% b5 w# bIndex .............................................................. 493作者: foxok 时间: 2015-8-9 16:47
