急求：从人血细胞诱导培养ips的protocol及文献

anning111

人血细胞有多种 细胞，红细胞没有细胞核，不能重编诱导，免疫细胞可以的，加入四个因了的慢病毒，再加入VC和VPA 换上ES 培养基就可以的，这个很成熟了，都差不多

anning111

Induction of pluripotent stem cells from fibroblast
6 u% H% Z8 R6 J- g! Rcultures
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( x) V$ ~9 {" H9 rKazutoshi Takahashi, Keisuke Okita, Masato Nakagawa & Shinya Yamanaka

anning111

行行行，我发几篇文章你看，
& B6 {( _' g! |4 r4 n" o1，Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, et al.
  B1 c9 J+ B/ j1 D& C; OEfficient human iPS cell derivation by a non-integrating plasmid6 R0 q$ k$ U7 P6 P
from blood cells with unique epigenetic and gene expression signatures.
/ u9 Z+ f  Y3 D8 {, J$ H8 vCell Res 2011; 21(3): 518-29.# K- F6 w! ^& @- K* |9 E. Q/ a
2Kim K, Doi A, Wen B, Ng K, Zhao R, Cahan P, et al. Epigenetic
& s) M! t! @, e) bmemory in induced pluripotent stem cells. Nature 2010;
$ c% j1 I) K5 ^! r( Z467(7313): 285-90.6 O6 n% i# ^7 M: c3 a# N
3Loh YH, Agarwal S, Park IH, Urbach A, Huo H, Heffner GC, et al.
4 W3 N, R* E0 P; FGeneration of induced pluripotent stem cells from human blood.  I. l. V0 X9 y, n! w
Blood 2009; 113(22): 5476-9.
) a$ U& W( E. q4Haase A, Olmer R, Schwanke K, Wunderlich S, Merkert S, Hess
6 }; n8 |/ ~- e7 qC, et al. Generation of induced pluripotent stem cells from human
) V5 q9 p4 F' R2 fcord blood. Cell Stem Cell 2009; 5(4): 434-41.
# U% g! C7 {; x5Giorgetti A, Montserrat N, Aasen T, Gonzalez F, Rodríguez-Pizà I,
9 P/ w0 ?/ {9 o$ \3 kVassena R, et al. Generation of induced pluripotent stem cells from
& Y5 l8 J! l0 ~/ }! K8 h/ whuman cord blood using OCT4 and SOX2. Cell Stem Cell 2009;4 r/ M; M0 z5 y" L3 \3 Q# X
记得多给我评点分哈，谢谢了，有什么问题可以问我哈
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