The cell debris are in the RNA samples? For RNA-seq, before library preparing, RNA need to be qualified first. Typically, using Agilent Bioanalyzer to do quality control. The RIN (RNA Integrity Number) should be greater than 8. Based on the kit you are using, at least 100ng of total RNA is needed. If your RNA RIN is good enough and you have much more amount of RNA than 100ng. Cell debris is not a problem.
The cell debris are in the RNA samples? For RNA-seq, before library preparing, RNA need to be qualified first. Typically, using Agilent Bioanalyzer to do quality control. The RIN (RNA Integrity Number) should be greater than 8. Based on the kit you are using, at least 100ng of total RNA is needed. If your RNA RIN is good enough and you have much more amount of RNA than 100ng. Cell debris is not a problem.
发表于 2016-10-20 03:50
